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Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Zoology 2024Quote: ... Protein concentration in each extract was measured using PierceTM BCA Protein Assay Kit (Thermo Scientific). Laemmli buffer (Biorad ...
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bioRxiv - Physiology 2024Quote: ... Samples with equal amounts of proteins were precleared with Dynabeads Protein G (Thermo Fisher Scientific) for 2 hours at 4 °C and then incubated with 3 µg of primary antibody on a rotary platform overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the Pierce BCA Protein Assay Kit (Cat#: 23227, Thermo Scientific). Samples were electrophoresed on polyacrylamide gels (Cat# NW04120BOX ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified by BCA Assay (Pierce™ BCA Protein Assay Kit, 23225; via ThermoFisher). Serum albumin was used as the standard.
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bioRxiv - Cell Biology 2024Quote: ... 4mM EDTA) and protein content was accessed using BCA Protein Assay (Thermo Fisher Scientific #23225). Then 5μg of protein was incubated with 1X Reaction Buffer ...
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bioRxiv - Genetics 2024Quote: ... Total protein concentrations of lysates were quantified by BCA Protein Assay (Thermo Fisher Scientific, #23225) and each sample was analyzed in technical duplicates for apo(a ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were measured with the Pierce 660 nm Protein Assay (Thermo Fisher Scientific, 22660) and 500 μg of protein per sample was used for subsequent mass spectrometry experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo-Fisher Scientific, Cat #23225). After concentration adjustment ...
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bioRxiv - Biochemistry 2024Quote: ... supernatants removed and protein concentration determined via the Pierce BCA Protein Assay Kit (Thermo Fisher). The absorbance of each sample was read at a wavelength of 562 nm (Infinite 2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the Pierce BCA protein assay kit (Thermo Scientific™, 23225). Following SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined using BCA assay (23225, Pierce™ BCA Protein Assay Kit, Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... We combined 4 mg of Cas9 Nuclease protein (TrueCut Cas9 Protein V2, Thermo Fisher Scientific), 5 mL of Neon Buffer R (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissolved protein was analyzed for protein concentration by micro-scale BCA assay (Micro BCA Protein Assay Kit #23225; Thermo Fisher Scientific Inc., Waltham, MA, USA). Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Bioengineering 2021Quote: ... 16 Protein concentration of the extracts was estimated using a BCA protein assay kit (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, Oakville, ON, Canada) as per supplier’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Microbiology 2019Quote: ... Sequences of recombinant RVAs were determined from cDNAs prepared from viral RNAs using a Superscript III reverse transcriptase kit (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... The drug-selected recombinant CHO-S cells were adapted to serum-free suspension culture using CHO-S medium (CD Forti CHO; ThermoFisher). For large-scale cultures ...
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bioRxiv - Neuroscience 2022Quote: ... After centrifugation, nuclei pellets were resuspended in 1 ml PBS-RI (PBS, 50 U/mL Rnase-OUT Recombinant Ribonuclease Inhibitor (Invitrogen), 1 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... the LRRK1RCKW plasmid was used for the generation of recombinant baculoviruses according to bac-to-bac expression system protocols (Invitrogen).
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bioRxiv - Biochemistry 2020Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium ...
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bioRxiv - Immunology 2021Quote: ... Individual B cells were sorted into 96-wells PCR plates (Bioké) containing 2 μL lysis buffer (0.2% (v/v) Triton X-100 + 2U/μL RNaseOUT™ Recombinant Ribonuclease (Invitrogen) in UltraPure™ DNAse/RNase free distilled water (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... Purified recombinant HisHA-INF1 or HisHA-Scr74 were incubated with 20 μl Pierce Anti-HA magnetic beads (Thermo Fisher Scientific) at an amount of 500 pmol each for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... crude extracts and purified recombinant SepF and FtsZ in serial dilution were loaded on a 4−12 % (w/v) BisTris PAGE gel (Invitrogen). The amounts of crude extract were 120 µg for FtsZ and 290 µg for SepF ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNAs of NPH3SA and NPH3SE within the pENTR vectors were transferred to both pH35GS and pH35YG binary vectors (Yamaguchi et al., 2008) using an LR recombinant reaction (Invitrogen). 35Spro:NPH3SA ...
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bioRxiv - Neuroscience 2019Quote: ... 59°C for 30s and 72°C for 30s using recombinant 5U/ul Taq polymerase prepared in 10X Buffer with 50mM MgCl2 (Invitrogen). The 388bp PCR amplicon was digested with 20U/µL EcoRV restriction enzyme prepared in 10X NEB 3.1 buffer (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Immunology 2022Quote: NK cells were enriched and incubated with 50 ng/ml recombinant mouse IL-15 (Preprotech) for four days in RPMI plus 10 % FBS with penicillin and streptomycin (Thermofisher) added in a U-bottom 96-well plate at the concentration of two million cells per ml ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids were transfected into Drosophila S2 cells using Calcium Phosphate Transfection Kit (Thermo Fisher Scientific, Paisley, Scotland, UK). Following antibiotic selection with Zeocin (InvivoGen ...
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bioRxiv - Microbiology 2020Quote: ... a recombinant bacmid DNA was generated from the sequencing-confirmed pFastBac construct using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... The bacmid was transfected into Sf9 cells to obtain recombinant baculovirus using Cellfectin II Reagent (Invitrogen Co., Carlsbad, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... fluorescent conjugate cholera toxin B (CTB; recombinant cholera toxin subunit B conjugated with Alexa fluorophores: 0.2% CTB-488 and CTB-594; Life Technologies) was injected into AL and PM.
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bioRxiv - Microbiology 2020Quote: ... the macrophages were allowed to adhere to the culture plate for 20 minutes before 100 units (U) of murine recombinant interferon-gamma (IFNγ) (Invitrogen) was added to each well to stimulate the macrophages ...
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bioRxiv - Immunology 2020Quote: ... A total of 100.000 T cells were co-cultured with 50,000 sorted myeloid cells in the presence of 30IU recombinant hIL-2 plus anti-CD3/CD28 Dynabeads (ThermoFisher Scientific) for 3-4 days in round-bottom plates ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... These recombinant cells expressing individual rod opsin variants were then surface immunostained with a Dylight 550-labeled anti-HA antibody (ThermoFisher) and then fractionated according to the immunostaining intensity of expressed variants using FACS ...
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bioRxiv - Cancer Biology 2019Quote: ... The recombinant vector and miR-659 mimic were jointly transfected into MDA-MB-231 cells using Lipofectamine™2000 (Invitrogen). These cells were lysed after 24 h of culture and the activity of luciferase was determined.
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bioRxiv - Microbiology 2019Quote: ... Eight μL of Cellfectin II reagent and 3 ng of each recombinant plasmid were diluted in 100 μL Grace’s medium (Thermofisher Scientific), respectively ...
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then activated by the addition of 0.2 μg/ml LPS and 0.1 ng/ml murine recombinant interferon-γ (IFNγ) (both ThermoFisher Scientific) for 24 h ...