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Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and 1% bovine serum albumin (BSA) and then incubated with biotinylated Epidermal Growth Factor (EGF) complexed to Texas Red™ Streptavidin (ThermoFischer/Molecular Probes Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed thrice with ice cold PBS2+ and surface BBS-DAT chimeras were labeled with 120μM biotinylated α-bungarotoxin (α-BTX-b, Thermo Fisher) in PBS2+ ...
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bioRxiv - Molecular Biology 2020Quote: ... The biotinylated DNA fragments were applied to the gel-shift assay by using the LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, US) and Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: The cell surface of KP4 cells and MYOF-HA expressing HEK293T cells was biotinylated on ice using 0.5 mg/ml of EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific: 21331) for 30min ...
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bioRxiv - Biophysics 2020Quote: ... The biotinylated surface was then incubated with FluoSpheres™ NeutrAvidin™-marked microspheres (200nm in diameter, fluorescent in yellow-green, ThermoFisher, F8774) with 1-5uL in 150uL PBS per hydrogel ...
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bioRxiv - Immunology 2020Quote: ... The protein concentration was determined and the antibodies biotinylated by incubating for 30 min at room temperature with EZ-Link NHS-PEG4-Biotinylation reagent (Thermo Fisher Scientific) at a 10:1 biotin-to-protein ratio ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-2 S was chemically biotinylated using EZ-Link™ NHS-LC-LC-Biotin following manufacturer instructions (Thermo Fisher, Cat#21343). Vero E6 cells (ATCC ...
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bioRxiv - Developmental Biology 2022Quote: ... The DNA was enriched for fragments containing a ligation junction by capturing biotinylated fragments on MyOne Streptavadin C1 magnetic beads (Thermo Scientific, 65001). The DNA ...
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bioRxiv - Immunology 2021Quote: ... 10,000 Hela-hACE2 cells generated by lentivirus transduction of wild-type Hela cells and enriched by fluorescence-activated cell sorting (FACS) using biotinylated SARS-CoV-2 RBD conjugated with streptavidin-Alexa Fluor 647 (ThermoFisher Scientific, S32357) were added to the mixture with 20 µg/ml Dextran (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... antibodies overnight at 4°C.The sections were then washed with PBS and incubated with a biotinylated secondary antibody for 15 min from a Histostain Plus kit (Invitrogen, CA, USA). The sections were then washed and incubated with 3 ...
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bioRxiv - Immunology 2021Quote: ... Washed yeast libraries were stained in dark and at 4°C with biotinylated antigens pre-conjugated with SA-PE (Streptavidin, R-Phycoerythrin Conjugate, Thermo Fisher Scientific) or SA-APC (Streptavidin ...
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bioRxiv - Biochemistry 2020Quote: ... and then with the appropriate biotinylated secondary antibodies for 30 minutes followed by streptavidin conjugated to Alexa Fluor 594 (ThermoFisher Scientific, UK) for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... and citrullinated Histone 4 using biotinylated peptides (Cit3-His4 bio: SG[Cit]GKGGKGLGKGGAKRHGSGSK-biotin) on streptavidin-coated high-capacity pre-blocked plates (Thermo Fisher Scientific) and hIgG1 at 5 μg/ml in RIA buffer (1% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... holo-Tf was biotinylated for two hours on ice with an EZ-LinkTM Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific) and then buffer exchanged into PBS with a 0.5 mL Pierce Protein Concentrator PES column with a 10K MWCO to remove unreacted biotin ...
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bioRxiv - Physiology 2024Quote: OK cells cultured on permeable supports under static or FSS conditions were washed with cold PBS and the apical surface biotinylated with 1 mg/mL EZ-Link Sulfo-NHS-SS-biotin (Thermo Scientific, 21331) in 0.5 mL TEA-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: Nanobodies specific for PfFIKK13149–561 were selected against biotinylated PfFIKK13149–561 immobilised on either Streptavidin-coated magnetic beads (Dynabeads™ M280-Streptavidin, ThermoFisher Scientific) or Pierce™ NeutrAvidin™ Coated plates (#15123 ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... The viruses in the bottom chamber were also detected by dot blot on a nitrocellulose membrane with a biotinylated anti-AAV9 antibody (Thermo Fisher, 7103332100).
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... and then with the appropriate biotinylated secondary antibodies for 30 minutes followed by streptavidin conjugated to Alexa Fluor 594 (ThermoFisher Scientific, UK) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... LC-MS/MS analysis of the biotinylated peptides was performed on an EASY-nLC 1200 UHPLC connected to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) as described previously67 ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with 80-fold molar excess of NHS-PEG4-Biotin solution from the EZ-Link™ NHS-PEG4-Biotin kit (ThermoFisher, #A39259). Excess biotin was removed by buffer exchange of protein into sterile 1X PBS using Zeba™ Spin Desalting Columns ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Microbiology 2023Quote: ... These PCR products were purified as previously and biotinylated DNA was incubated for 4 hours with streptavidin beads (Dynabeads® kilobaseBINDER™, Invitrogen) to capture only DNA fragments containing the transposon ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed in three 10-min PBS exchanges and underwent tyramine signal amplification by treating with PBT comprising 0.005% hydrogen peroxide and 0.5% borate-buffered biotinylated (Sulfo-NHS-LC biotin; 21335, Thermo Fisher Scientific, Waltham, MA) tyramine (T90344 ...
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bioRxiv - Immunology 2023Quote: ... using a Superdex S75 10/300 column in 1XPBS and chemically biotinylated using EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific) following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Only those sections incubated with biotinylated secondary antibodies were next incubated with Streptavidin-Alexa Fluor 488 conjugate 1:1000 (ThermoFisher scientific, S32354) in PBS with 0.1% Triton X100 ...
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bioRxiv - Biochemistry 2024Quote: ... The desthio-biotinylated mCNPase and hCNPase were immobilised for 2 h at RT on streptavidin-coated flat-bottom 96-well plates (Thermo Fisher Scientific). The wells were washed three times 10 min with PBS and blocked using 5% skim milk in PBS-T for 3 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: DNA probes were biotinylated at the 3’terminal using Biotin 11-dUTP (Thermo: R0081) and terminal deoxynucleotidyl transferase TdT enzyme (Thermo scientific: EP0161) in the presence of CaCl2 containing 5X reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The final products were size-selected one last time and rRNA depletion was performed using custom-made biotinylated oligos(38) (Table 3) together with MyOne Streptavidin C1 DynaBeads (Thermo Fisher Scientific). Purified RPFs were then used to synthesize cDNA with SuperScript III (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 pmol of synthetic biotinylated RNA or single-stranded DNA (poly(dA) or poly(dG)) were loaded onto the Pierce Streptavidin Magnetic Bead (Thermo Scientific, #20164), and an experimentally normalised 5 µg of human recombinant TG2 was used (this resulted in readily detectable signal after the pull-down).
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bioRxiv - Immunology 2024Quote: The oligonucleotides for the mouse Srebp1a promoter and their complementary strands were synthesized by GenePharma (Shanghai, China) and biotinylated using a Pierce™ Biotin 3’ End DNA Labeling Kit (Cat. #89818; Thermo Scientific). These oligonucleotides were annealed to form double-stranded oligonucleotides ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... Human kidney cells (HKC) and human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. # 11965126). Mouse embryonic fibroblasts (MEF ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
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bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
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bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
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bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
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bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...