Labshake search
Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Cultures were then diluted 1:1.000 in fresh medium and 200 μl were transferred into 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 15 min for 16 h using a Biotek Synergy HTX plate reader (Agilent) ...
-
bioRxiv - Immunology 2023Quote: ... Bone marrow was extracted from the femur and tibia of dissected mice and plated on non-tissue culture-treated 15-cm Petri dishes at a density of 1 x 107 cells per plate in macrophage differentiation medium (DMEM, Gibco) supplemented with 2mM L-glutamine ...
-
bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
-
bioRxiv - Neuroscience 2024Quote: ... in 6-well tissue culture plates containing 1 ml prewarmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
-
bioRxiv - Immunology 2024Quote: Bd isolate JEL 197 was grown in 1% tryptone broth or on 1% tryptone agar plates (Difco Laboratories, Detroit, MI) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco) at 19°C.
-
bioRxiv - Physiology 2024Quote: ... Monocytes were cultured in 12-well plates at a density of 1 × 106/mL in Roswell Park Memorial Institute media 1640 (Gibco) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Cell Biology 2024Quote: ... Biotinylated TACC3 Δ699-765 (50 µL of 10 µg mL−1, diluted in PBS) was immobilized on preblocked HBC Streptavidin plates (ThermoFisher) for 30 min at room temperature (RT) ...
-
bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... into a single-cell suspension and seeded on Matrigel-coated plates with ROCK inhibitor and knockout serum replacement (1%, Gibco).
-
bioRxiv - Microbiology 2024Quote: ... cells were seeded in a 24-well plate at a density of 105 cells per well in 1 ml MEM (Gibco) and grown for 5-6 days ...
-
bioRxiv - Microbiology 2024Quote: ... Vero cells (1 × 105 cells per well) in a 24-well cluster plate were transfected with 1 μg plasmid DNA using the Lipofectamine 3000 system (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... After another rinse and three more washes the plate was developed with 1-Step™ Ultra TMB-ELISA Solution (ThermoFisher) before stopping the reaction with an equal volume of 2M H2SO4 ...
-
bioRxiv - Plant Biology 2020Quote: ... The amplicons were checked by electrophoresis and 25 µl of each pooled PCR product were purified and normalized using SequalPrep Normalization Plate Kit (Invitrogen GmbH, Karlsruhe, Germany). We then pooled the samples and the mock community and proceeded for a single library preparation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing reactions were carried out on 96-well plates using BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems, foster City, CA) for 30 cycles ...
-
bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from 10-day old seedlings grown on ½ MS plates under long-day conditions using PureLink RNA Mini Kit (Thermo Fisher Scientific, 12183025). One microgram of total RNA was reverse transcribed into cDNA with Protoscript II (New England Biolabs ...
-
bioRxiv - Microbiology 2021Quote: ... as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat. No. A1051001, ThermoFisher, Waltham, MA, USA) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... cDNA was produced from mRNA from cells lysed directly in the culture plate using the TaqMan® Gene Expression Cells-to-CT™ Kit (ThermoFisher Scientific). qRT-PCR was then carried out using the corresponding TaqMan™ Gene Expression Assay Kit that include exonspanning probes for genes of interest (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 37°C for 24 hr and stained with a Filmtracer LIVE/DEAD Biofilm Viability Kit (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
-
bioRxiv - Genomics 2020Quote: 10 μL of PCR product for each sample was normalized using a SequalPrep 96-well Normalization Plate Kit (Thermo Fisher Scientific, Waltham, MA). Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter ...
-
bioRxiv - Immunology 2022Quote: DNA in 100 µl of undiluted cell-free BALF (first wash) was quantified in DNase-free 96-well plates (TPP®) using Quant-iTTM Pico GreenTM dsDNA Assay Kit (Thermo Fisher Scientific) following manufacturers’ instructions.
-
bioRxiv - Zoology 2019Quote: Ten microliters of PCR amplicon was purified and normalized for concentration prior to library preparation using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, USA). DNA libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs ...
-
bioRxiv - Genomics 2022Quote: ... and split half for maintenance in a well of a 96-well plate and half used for genotyping using the Phire Animal Tissue Direct PCR Kit (Thermo Fisher Scientific, F140WH) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 µL of each concentration was transferred into a U-shaped 96-well plate and incubated with 100 µL QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™) for 40 minutes along with the sEV samples to be detected on O-NEXOS ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 9 µL of the dPCR reaction mix was added to each well of a QuantStudio Absolute Q MAP16 Plate Kit (PN A52865, ThermoFisher Scientific, Waltham, MA). Next ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... for final quality assurance the CFFT Lab subjected all its plasmids from the daughter plate to next generation sequencing (NGS): DNA from plasmid constructs was quantitated using Qubit dsDNA HS Assay Kit (Q33230, Thermo Fisher Scientific, USA) as per manufacturer instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Raw cytokine values per well were normalized over nuclei number per well based on fluorescent staining of the fixed iAstrocytes in the plate or over protein content per well determined by Pierce™ BCA protein assay kit (Thermo Scientific, 23225). Pierce™ BCA protein assay was performed in a microplate as described in the user guide provided by Thermo Scientific.
-
bioRxiv - Cell Biology 2024Quote: ... was used to measure collagen in lung lobes following kit instructions and optical density was measured using a Varioskan LUX plate reader (Thermo Fisher Scientific, VL0000D0).
-
bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
-
bioRxiv - Neuroscience 2024Quote: 1 μL of RNA was assayed using the TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher, #4392938). Specific transcripts were detected using Taqman assays ...
-
bioRxiv - Biophysics 2021Quote: ... blot time: 3 s) and plunged frozen into liquid ethane using a Vitrobot Mark IV system (ThermoFisher).
-
bioRxiv - Microbiology 2019Quote: ... by electrophoresis (Compact XS/S, Biometra) and DNA bands were visualized with ethidium bromide (Thermo Fisher Scientific) under ultraviolet (UV ...
-
bioRxiv - Neuroscience 2021Quote: ... Microarray studies were performed using Affymetrix GeneChip Mouse Clariom S arrays (Thermo Fisher Scientific, Hampton, NH, USA). Gene expression analysis was performed using iDEP (integrated Differential Expression and Pathway analysis ...
-
bioRxiv - Molecular Biology 2019Quote: ... Reverse primer; CCCATGGTGACAGTCTTGAAGA. Promoter S. Region (−189 to −45. Probe; ACCTGAAGGACTCTTG with an MGB quencher (Taqman, Invitrogen), Forward primer ...
-
bioRxiv - Immunology 2021Quote: ... ChAdOx1 SARS CoV-2 S viral vectors were rescued in T-REx™ cells (Invitrogen, Cat. R71007), a derivative of HEK293 cells which constitutively express the Tet repressor protein and prevent antigen expression during virus production ...
-
bioRxiv - Biophysics 2021Quote: ... and Kappa variants were individually cloned into the mammalian expression vector pcDNA3.4-TOPO (Invitrogen, U. S. A.), which contains a foldon trimerization domain based on phage T4 fibritin followed by a c-Myc epitope and a hexa-repeat histidine tag as previously described32,42 ...
-
bioRxiv - Cell Biology 2021Quote: ... For live imaging of cells as described for fatty acids up-take we treated cells at day 5 of differentiation with BODIPY™ (10μM) C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen). In addition ...
-
bioRxiv - Immunology 2020Quote: ... 50 U/ ml penicillin and 50 μg/ml streptomycin (P/S; (Gibco, Invitrogen, Grand Island, NY, USA). They were incubated at 37°C (with humidity ...
-
bioRxiv - Molecular Biology 2021Quote: ... L-glutamine (5 mg/ml) in the presence 4 μg/ml Blasticidin S HCl (A11139-03, Invitrogen) and 35 μg/ml Zeozin (R250-01 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Copy DNA was synthesized using the SuperScript II first-strand-synthesis method with oligo(dT)s (Invitrogen) and analyzed using TaqMan gene expression assays (TaqMan Assay ID Hs00170751_m1) ...
-
bioRxiv - Biochemistry 2021Quote: The SARS-CoV-2 S ectodomains were produced in 200 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... for 3–5 s and then washed thoroughly in phosphate-buffered saline (PBS) (pH 7.4, Gibco, USA). Single zona pellucida-free embryos were carefully pipetted and placed into individual tubes containing 2 μL PBS ...
-
bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
-
bioRxiv - Immunology 2019Quote: ... 100 U/ml of penicillin and 100 μg/ml of streptomycin (P/S; Life Technologies; 15140-122), and 1× non-essential amino acids (NEAA ...
-
bioRxiv - Bioengineering 2019Quote: ... intracellular lipid vacuoles and nuclei were stained using the neutral lipid stain BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene; D3922, Thermo Fisher Scientific) and 4′,6-Diamidin-2-phenylindol (DAPI;D8417 ...
-
bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated on the grid for 30 s in a Vitrobot MarkIV (Thermo Fisher Scientific) at 4°C and 100 % humidity and subsequently blotted for 3 s seconds with a blot force of 0 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 249 S/R were cultured in Dulbecco’s modified Eagle’s medium with 4.5 g/l glucose Glutamax-I (Invitrogen) supplemented with 10% fetal calf serum (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2021Quote: ... Transgenic parasites were selected with 4 nM WR99210 (Jacobus Pharmaceuticals) or 2 μg/ml blasticidin S (Invitrogen). To select for integrants by SLI ...
-
bioRxiv - Immunology 2020Quote: ... 50 U/ ml penicillin and 50 μg/ml streptomycin (P/S; (Gibco, Invitrogen, Grand Island, NY, USA). They were incubated at 37°C (with humidity ...
-
bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 100 U/ml penicillin/streptomycin (P/S, Life Technologies) For knock-down experiments ...