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Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: AML and MDM cells were fixed with 4% formaldehyde and 1% glutaraldehyde in phosphate buffer (Invitrogen) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or Caspase-1 over-night 4°C and with fluorochrome-conjugated secondary antibody (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked in 1% BSA/0.1% saponin-PBS with 4% horse serum (26050-070, Gibco) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... at 1% B with Solvent A as 0.1% formic acid in water (Thermofisher Optima LS118-4) and Solvent B as 0.1% formic acid in acetonitrile (Thermofisher Optima LS120-4) ...
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bioRxiv - Neuroscience 2023Quote: ... for 72 h at 4°C and secondary antibody anti-mouse Alexa-488 (1:300, Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation overnight at 4°C with secondary antibodies (1:1000, Alexa-Fluor conjugated, Invitrogen). Following incubation in secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were incubated overnight at 4°C using TM4SF1 (ThermoFisher Scientific #PA5-21119, 1:200) and CD31 (Dako #M0823 ...
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bioRxiv - Cancer Biology 2024Quote: ... An α-Mouse IgG2a-AF488 secondary antibody (Invitrogen, CAT#: A-21131; 1-4 hours, 25°C), cells were washed 3 times ...
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bioRxiv - Immunology 2024Quote: ... Th1 consisted of 1 µg/mL anti-murine IL-4 (Cat# 16-7041 Invitrogen Carlsbad, CA), 5 ng/mL murine IL-2 (Cat# 212-12 Peprotech Cranbury ...
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bioRxiv - Immunology 2024Quote: ... These were transfected at a 1:2:4 w/w ratio using Lipofectamine 3000 (Invitrogen #L3000001), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then 1 h at 4°C at 7-10 rpm with dynabeads protein A (Invitrogen), equilibrated with the EB ...
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bioRxiv - Molecular Biology 2020Quote: ... (G4S)6 Linker-VH/CH1 and (G4S)6 Linker-VH/CH1-(G4S)6 Linker-VH/CH1 sequences were synthesized using GeneArt® gene synthesis service (ThermoFisher Scientific).
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bioRxiv - Genomics 2021Quote: ... cells were seeded in 6-well plates or 6 cm dishes and reverse transfected with 5 nM Silencer Select siRNAs (Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... iPSC cultures at ~90% confluence in 6-well-plates were treated with 6 μM CHIR-99021 (SelleckChem) in RPMI 1640 medium supplemented with B27 supplements (Thermo Fisher Scientific) for 2 days to induce mesoderm specification ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... was co-transfected with either pre-gRNA or gRNA expression plasmids (1 µg per 6-well) using Lipofectamine 3000 kit (5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were first transfected with the pcDNA3_CasRx-GFP plasmid (2.5 µg per 6-well) using Lipofectamine 3000 kit (5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2019Quote: ... were made up in HPLC grade H2O (1 mL) immediately before injection onto a ProPac PA-1 analytical column (4 × 250 mm, ThermoFisher Scientific, UK), pre-equilibrated in HPLC grade H2O at a flow rate of 1 mL.min-1 ...
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bioRxiv - Neuroscience 2020Quote: ... OFC sections were reacted with the primary antibody for rabbit met-enkephalin (1:400, Abeam ab22620) for 24h at 4°C and Alexa-fluor 488 goat anti rabbit secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) supplemented with 0.1 g/l of SDS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... (alone or in combination) in IF washing buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Collagen/laminin coating was accomplished by overnight incubation of the plates rocking at 4°C with a 1:1 mixture of laminin (10 µg/ml ThermoFisher 23017-015) and collagen (10 µg/ml Sigma C8919 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was washed 5 times 5 min in TBS-T prior to overnight incubation at 4°C with 1° anti-Flag antibody (1:500, ThermoFisher, PA1-984B). The next morning ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Microbiology 2022Quote: ... the Transwell inserts were incubated with one of the following primary Abs in IF wash buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (40-2200, Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Neuroscience 2023Quote: ... concentration 1:100) for 24 h at 4°C followed by labeling with goat anti-rabbit Alexa fluor 488 (A11034, Invitrogen, 1:1000) as a secondary antibody for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2023Quote: ... and 20% sucrose-PBS and then incubated overnight at 4°C in a 1:1 solution of 20% sucrose and OCT compound (Fisher Scientific: #23730571). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... were mechanically lysed in [100 μl/sample + 10 μl extra] lysis buffer (100 mM NaCl, 50 mM Tris, 4 mM EDTA, 1% NP40, 1 μl/ml SUPERaseIN (Thermo Fisher #AM2694), and 1x cOmplete Protease Inhibitor (Roche #11836153001) ...
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bioRxiv - Genomics 2023Quote: ... 2.0 mM 2-mercaptoethanol and 0.1 mg/mL-1 bovine serum albumin) and then stopped by adding 4× LDS sample buffer (∼3.3μL) (Thermo Fisher Scientific, no. NP0007) to a final concentration of 1× (final volume ∼13.3μL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining was performed at 1:200 for 30 minutes prior to fixation overnight in 1% phosphate buffered formalin (Fisher Scientific, SF100-4). Note ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies were detected using secondary antibodies (diluted 1:500-1:1000 in PBS/4% BSA) conjugated to Alexa Fluor 568 or 647 (Thermo Fisher Scientific). Cells were counterstained with DAPI and kept in PBS until images were taken with the LSM780 microscope.
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bioRxiv - Immunology 2022Quote: Caspase-3 activity was determined using EnzChek™ Caspase-3 Assay Kit #2 (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... (75 mm i.d. 3 2 cm, Acclaim PepMap100 C18 3 mm, 100 A°, ThermoFisher Scientific) and separated over an EASY-Spray column ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Biophysics 2023Quote: ... 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine (DiI) and 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine (DiD)) were purchased from ThermoFisher Scientific and used without additional purification ...
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Biophysics 2020Quote: A 6-well plates (Nalge Nunc International, Roskilde, Denmark) was treated with Poly-L-Lysine (PLL ...
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bioRxiv - Cell Biology 2020Quote: ... and 1.5 μg Random Primer Pd(N)6 (Invitrogen). RNA was denatured at 65°C for 2 min and then added to 40 U RNAse inhibitors (RNaseOUT-Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... media was changed to Essential 6 Medium (Gibco; A1516401), 24 hours later cells were treated with 200ng/ml FGF8b (PeproTech ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Invitrogen, Eugene, OR, USA). Quantification of GFAP ...
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bioRxiv - Neuroscience 2019Quote: ... Transient transfection was performed with Fugene 6 reagent (Invitrogen) according to the manufacturer’s instructions.