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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... (5’-CUAUGAACAUAAAGUCUGCTT-3’) or its non-specific control (5’-UUCUCCGAACGUGUCACGUTT-3’) were transfected into cells using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA).
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bioRxiv - Neuroscience 2021Quote: ... Drosophila dSF2 sequence was PCR amplified (dSF2 F 5’-CACCATGGGATCACGCAACGAGTGCCG-3’ and dSF2 R 5’- ATAGTTAGAACGTGAGCGAGACCTGG-3’) was cloned into pEntry-TOPO vector (Thermo Fisher Scientific). The pENTR-dSF2 vector was recombined with Gateway plasmid pTWH (Drosophila Genomics Resource Center ...
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bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Microbiology 2022Quote: ... The 16S rRNA gene was amplified with the primers 8f (5′-AGAGTTTGATCCTGGCTCAG-3′; Weisburg et al. 1991) and 1520 r (5′-AAGGAGGTGATCCAGCCGCA-3′; Edwards et al. 1989) (Invitrogen, CA, USA). A volume of 0.5 μL DNA extract was used for 50 μL PCR reactions containing 2 units Taq DNA polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... The following day the miR-423-5p or miR-Ctrl vector was cotransfected with either the Cacna2d2-3’UTR or the Cacna2d2 mut 3’UTR reporter vectors using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (BODIPY FL DHPE) were purchased as solids from Invitrogen (Thermo Fischer Scientific), Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Microbiology 2024Quote: ... and a pan VSG reverse primer which binds to a conserved 14bp region of the 3’ UTR (5’-GATTTAGGTGACACTATAGTGTTAAAATATATC-3’) with AmpliTaq Gold (Applied Biosystems, 4398881) (anneal & extension 60C 1m ...
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bioRxiv - Microbiology 2024Quote: ... Purified cDNA was amplified for 25 cycles with VSG specific primers: a spliced-leader (5’-ACAGTTTCTGTACTATATTG-3’) and SP6-VSG 14-mer (5’-GATTTAGGTGACACTATAGTGTTAAAATATATC-3’) using Phusion polymerase (Thermo Scientific, F530L) (annealing temp 55C ...
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bioRxiv - Microbiology 2024Quote: ... 2uL of RNase-treated cDNA was amplified for 35 cycles with VSG specific primers: a spliced-leader (5’-ACAGTTTCTGTACTATATTG-3’) and SP6-VSG 14-mer (5’-GATTTAGGTGACACTATAGTGTTAAAATATATC-3’) using Phusion polymerase (Thermo Fisher, F530L) (annealing temp 55C ...
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (95°C for 3 seconds, 60°C for 30 seconds) on a QuantStudio 3 (Applied Biosystems, MA).
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bioRxiv - Cell Biology 2023Quote: Supernatants of cell culture medium collected during medium exchanges were analysed for albumin (10242, Diagnostic Systems) and 3-beta-hydroxybutyrate (Autokit 3-HB, Fujifilm Wako) on an Indiko Plus chemical analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Physiology 2023Quote: ... Digitonin solubilized mitochondria were loaded 3-12% Bis-Tris Invitrogen™ Novex™ NativePAGE™ 3-12% acrylamide gel (1mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of LotA7-544 was loaded onto a glow-discharged grid and blotted for 3 s with blot force 3 using Vitrobot Mark IV (Thermo Fisher, U.S.A.).
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bioRxiv - Neuroscience 2023Quote: ... Neuron-cancer cocultures were quantified for neuronal outgrowth by identical image generation and processing with additional pre-incubation (3 drops/mL for 90 min at 37◦C) of NucRed Dead 647 ReadyProbes™ Reagent (TO-PRO-3 iodide) (Invitrogen, #R37113) for dead cells and NucBlue Live ReadyProbes™ Reagent (Hoechst 33342 ...
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bioRxiv - Cell Biology 2024Quote: ... For SNX5 and SNX6 the following siRNA sequences were used: SNX5 (5′-UUAGUUUCAGCCCGAAGCAUC-3’), SNX6 (5′-UUAUGAGGUAGACGACUAAAU-3’) (Wassmer et al., 2007), VPS35 (ID 132357, AM16708) (Predesigned – Thermo Fisher Scientific). Nontargeting control siRNA (control ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was harvested from a 10 ml parasite culture (5% hematocrit; >3% parasitaemia) by resuspending the pelleted RBCs in 3 ml pre-warmed Trizol (Invitrogen #15596-018). After a 5 min incubation step at 37°C ...
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bioRxiv - Neuroscience 2021Quote: DRGN and retinal cells were washed with PBS and total RNA (n = 6-9 wells/treatment) was extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen). Appropriate primers were used to validate each gene of interest using the specific primers (Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Molecular Biology 2024Quote: WT and TRBP−/− HeLa cells were plated at 4 x 106 cells / dish into a 9-cm dish and transfected with 6 µg/dish poly(I:C) using Lipofectamine 2000 (Invitrogen). At 0 ...
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bioRxiv - Immunology 2021Quote: ... After 3 consecutive washes anti-rabbit Alexa Fluor-568 conjugated antibodies (1:500; Thermo Fisher Scientific) were applied for 2 h before counterstaining with DAPI.
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bioRxiv - Microbiology 2020Quote: ... Parasitemias were determined on day 3 by staining with SYBR Green 1 nucleic acid stain (Invitrogen) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... slices were further incubated with TO-PRO-3 Iodide (diluted in PBS, 1:2000, Life Technologies) at RT for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... then 500 μL volumes of blood were diluted in sterile PBS (1:3) (BR0014G, Thermofisher Scientific) and centrifuged at 500xg for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... at a ratio of 1:3 of DNA: PEI in 200 μl of opti-MEM (Invitrogen) (Wang ...
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bioRxiv - Neuroscience 2020Quote: ... or 3) Goat anti-rabbit conjugated with Alexa Fluor 647 (1:1000) (Life technologies, Carlsbad, CA) for 2 hours at room temperature protected from light ...
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bioRxiv - Cell Biology 2020Quote: ... 3-NP-induced cytotoxicity was measured by fluorescence using the die YOYO-1 (#Y3601, Life Technologies). Treated cells were placed in an Incucyte FLR imaging system and the YOYO-1 fluorescence was measured after several time points ...
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bioRxiv - Bioengineering 2022Quote: ... in a ratio of 1:3 with a supplement of H2O2 (30%, Certified ACS, Fisher Scientific) at 200 °C for 50 min in a microwave digestion system (Titan MPS ...
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bioRxiv - Microbiology 2021Quote: ... were mixed at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were allowed to adhere for 1-3 hours before media was changed to Neurobasal (Invitrogen) supplemented with 1x B27 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: RNA from 1-3 x 106 cells was extracted and purified with TRIzol reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2016) are presented in Table 1 using an Applied Quant Studio 3 (Applied Biosystems, CA, USA). For PKoV RNA and PAstV-1 RNA detection ...
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bioRxiv - Microbiology 2020Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 µg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... and the nuclei were stained with TO-PRO®-3 1:1000 (Thermo Fisher Scientific, USA) for 10–20 min ...
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bioRxiv - Microbiology 2021Quote: ... at a 1:3 ratio (mg DNA/mg PEI) mixed in Opti-MEM (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... The sections were counterstained with TO-PRO™-3 (T3605, Invitrogen, OR, USA; dilution 1:1000) and examined using a confocal laser microscope (DMi8 ...
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bioRxiv - Genetics 2019Quote: ... except that 1-Bromo-3-chloropropane was used instead of chloroform and DNAse (TURBO DNase, Ambion) treatment was carried out ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and transfected with 1:3 ratio (RNA:Lipofectamine) using Lipofectamine RNAiMAX reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: The stock solutions for the GLVs (Z)-3-hexen-1-ol (Z3-HOL, 98%; Acros Organics) and (Z)-3-hexenyl acetate (Z3-HAC ...
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bioRxiv - Immunology 2024Quote: ... and a 3:1 CD3/CD28 mouse T-activator Dynabead to Treg ratio (Thermo Fisher Scientific). On day 3 of expansion ...
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bioRxiv - Cell Biology 2024Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...