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Citations for Thermo Fisher :
4651 - 4700 of 7736 citations for Recombinant Human TNFRSF4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney293 (HEK293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta biologicals ...
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bioRxiv - Biophysics 2021Quote: ... The human Gβ1 and Gγ2 subunits were integrated into the pFastBac Dual vector (Invitrogen) with an N-terminal 6 × His-tag at the N terminus of Gβ1 ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher, Waltham, MA) at a 2:1 bead:cell ratio for 48 h prior to electroporation ...
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bioRxiv - Cancer Biology 2019Quote: ... and two independent human GRPR siRNAs (Santa Cruz, # sc-106924 and Life Technologies, #145216), human GRPR shRNA in Lentiviral Vector (Genomics Online ...
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bioRxiv - Cell Biology 2021Quote: Undifferentiated human neuroblastoma SH-SY5Y cells were cultured in DMEM/F12 medium (Gibco, 11320033) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Rb polyclonal antibody anti-GMAP-210 (proteintech) or Ms monoclonal anti-Trip230 human (Invitrogen). Images were acquired on a Leica confocal TCS SP8 STED microscope ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1ẞ high sensitivity kit sold by Invitrogen. The sample for our ELISA consisted of cell culture media (referred to as sample hereafter ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of 5 μg/mL fibrinogen from human plasma conjugated to AF488 (Invitrogen) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... anti-human CD20 mouse IgG2 monoclonal antibody clone 2H7 (Invitrogen, cat: 14-0209-82), anti-human CD38 chimeric monoclonal antibody kindly provided by Centrose LLC ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-human and anti-mouse antibodies pre-coupled to Alexa Fluor 647 (Invitrogen) were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Microbiology 2021Quote: ... A mating strategy was used to screen a commercial human spleen cDNA library (Invitrogen) established in the pPC86 vector to express cellular proteins in fusion downstream of the GAL4 transactivation domain (GAL4-AD) ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... Human DDX42 cDNA was amplified by RT-PCR using the SuperScript III™ (Invitrogen) from mRNAs of MDMs using primers DDX42-forward 5’-AATTAATTTAGGATCCATGAACTGGAATAAAGGTGGTCCTG and DDX42-reverse 5’-AATTAATTTACTCGAGCTAACTGTCCCATCGACTTTTCTTGCG ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from mouse and human atrial tissue was extracted using Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The Taqman probes used for human and mouse genes were purchased from Applied Biosystems and listed in Table S5.
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bioRxiv - Microbiology 2019Quote: ... Human lung epithelial A549 cells were maintained in Gibco’s F-12 medium (Life Technologies). MDCK and A549 cells were obtained from Dr ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from human breast tissue after homogenization into TRIzol (Ambion, Life Technologies) using Qiagen Tissue Lyser II with metal beads ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from human breast tissue after homogenization into TRIzol (Ambion, Life Technologies) using Qiagen Tissue Lyser II with metal beads ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human lung fibroblasts were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing libraries were generated using Ion AmpliSeq Transcriptome Human Gene Expression Panel (ThermoFisher Scientific). Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... FITC conjugated F(ab’)2 goat anti-human IgA (Thermo Fisher Scientific, Illkirch, France), Brilliant Violet 650 conjugated streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Human and mouse IL-1β secretion was quantified by ELISA kits (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... followed by coating with antibodies: anti-human CD3 (clone OKT3, 10 μg/mL, Invitrogen) and anti-human CD28 (clone CD28.2 ...
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bioRxiv - Cell Biology 2022Quote: RNAs were collected from the human Sertoli cells using TRIzol (Life Technologies, Waltham, MA) and cDNA was synthesized ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were mixed with Human T-activator CD3/CD28 DynaBeads (Thermo Fisher Scientific) in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... was performed using the cDNA of human GPRC6A cloned in the vector pcDNA3 (Invitrogen) as template ...
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bioRxiv - Bioengineering 2021Quote: ... anti-CD9 (1:500, mouse monoclonal anti-human CD9, clone MM2/57, Invitrogen, AHS0902). The membranes were then washed three times with 0.1% Tween 20 in PBS for 5 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... The goat anti-human IgG conjugated with Alexafluor-647 was purchased from Invitrogen (A21445).
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bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
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bioRxiv - Immunology 2022Quote: ... the following primary antibodies were used: CD69 mouse anti-human (#MA5-15612 Thermo Fisher), IFN-alpha rabbit polyclonal (#PA5-115430 ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... T cells were activated by adding Dynabeads Human T-Expander CD3/CD28 (Invitrogen, 11141D) at a bead:PBMC ratio of 3:1 for 48 hours in a 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... Relative mRNA levels were determined using the ΔΔCt method using human RNASEP (Applied Biosystems) as internal reference ...
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bioRxiv - Immunology 2022Quote: IFNγ in supernatant was measured using Human IFNγ ELISA Kit (Invitrogen, 88-7316-88) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Human iPSCs were generated using the CytoTune-iPS 2.9 Sendai Reprogramming Kit (Invitrogen; A16157) as previously described47,61 ...
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bioRxiv - Immunology 2022Quote: ... then 80µL of secondary anti-human Igκ light chain HRP-conjugated antibody (ThermoFisher, A18853) diluted 1:1000 in 0.1% BSA-PBS was added ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan RT primers against human occludin (Hs05465837_g1) and GAPDH (Hs02786624_g1) were purchased from Invitrogen.
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bioRxiv - Microbiology 2021Quote: ... human bronchial tissue was cut into small pieces in Hank’s buffer (Thermo Fisher Scientific) containing 0.18% protease XIV and incubated for two hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Human foreskin fibroblast (HFF) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco), Raw264.7 cells were grown in DMEM (Gibco ...
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bioRxiv - Neuroscience 2019Quote: Human epithelial kidney (HEK 293T) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Gibco) supplemented with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 633-conjugated goat anti-human antibody (1:500; A21091 from Life Technologies); Alexa Fluor 555-conjugated goat anti-rabbit (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations for human cytokines were determined using the ProcartaPlex Multiplex Immunoassay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 0.05 mM calcium chloride and human keratinocyte growth supplement (Gibco cat. S0015). HaCaT cells and NIH3T3 cells were cultured in DMEM/high glucose (HyClone cat ...
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bioRxiv - Immunology 2020Quote: ... T-cells were incubated with murine or human anti-CD3/CD28 Dyna Beads (ThermoFisher) for 48 hours unless noted otherwise ...
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bioRxiv - Cell Biology 2021Quote: Human HEK293 suspension culture cells (Expi293F) were cultured in Expi293F expression media (Fisher Scientific) and maintained at 37°C in 8% CO2 in 125 mL spinner flasks on an orbital shaker rotating at 125 rpm ...
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bioRxiv - Microbiology 2020Quote: ... and Alexa Fluor 647 conjugated goat α-human IgG (ThermoFisher Scientific, 2.5 μg/mL), for 1 hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from human osteoclasts using the TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher, # 111.32D).