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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... HET(1) and HET(2) hESCs was extracted using mirVana microRNA isolation kit (ThermoFisher). Small RNA libraries were generated using NEXTFlex Small RNA Library Prep Kit v3 (Cat #NOVA-5132–06 ...
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bioRxiv - Molecular Biology 2024Quote: ... using Power SYBR® Green RNA-to-CT™ 1-Step Kit (Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... medium was removed and cells were lysed directly on plate in RNA lysis buffer and processed using the MagMAX® mirVana Total RNA Isolation Kit (Thermo Fisher Scientific Inc., Cat. No. A27828) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... RNA samples were diluted 1:100 prior to RT-qPCR with Power SYBR Green RNA-to-Ct 1-step kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... F2 progeny of all crosses were screened for fzo-1(tm1133) and/or pdr-1(gk448) mutations using a single worm PCR Phire Animal Tissue Direct PCR Kit (Thermo Scientific). The resultant amplification products were visualized by gel electrophoresis ...
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bioRxiv - Bioengineering 2021Quote: ... solution (1 mg/mL in DMEM) with 1:50 Cy5 dye-labeled fibronectin (conjugated in house using dialysis kit from Life technologies) was added on the chip and incubated for 1 h at room temperature ...
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bioRxiv - Pathology 2022Quote: ... 40 μL of elution buffer (from the kit) and 20 μL of 1:1 NuPAGE sample reducing agent (Thermo Scientific, NP0009) and NuPAGE LDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and 1 million cells were incubated with 1:100 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Life Technologies), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 8% CO2 with 125 rpm rotation to a density of 3.0 x 106 live cells per mL of culture were transiently transfected with an appropriate plasmid at 1 μg of DNA per 1 mL of culture using the Expifectamine transfection kit (Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then covered with a mix of 1 ml of Reagent A and 1 ml of Reagent B from a Pierce ECL western blotting substrate kit (Thermofisher, 32106). Excess liquid was removed ...
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bioRxiv - Microbiology 2020Quote: ... were grown overnight in 600 μL of LB at 37°C in a 1 mL-volume 96-deep-well plate (Thermo Scientific; catalogue number 260251), aliquoted in 96-well plates with 15% glycerol ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Bioengineering 2021Quote: ... The sections were cultured statically in a 24 well plate and incubated for and additional hour before adding cell culture media (DMEM, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA) for 5 days ...
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bioRxiv - Cell Biology 2021Quote: Plasmid transfection into NIH3T3 cells was carried out with 500 ng DNA per well of a 24-well plate using 1 µl Lipofectamine LTX (Thermo Fisher Scientific, Cat# 15338100) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: INS-1E or INS-1 832/13 cells were seeded in a 12-well plate and transfected with siRNA using RNAiMAX (Thermo Fisher Scientific, Cat# 13778150). ON-TARGET plus SMARTpool siRNA was used for knocking down rat Wfs1 (Dharmacon ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid transfection into ARPE-19 cells was carried out with 500 ng DNA per well of a 24-well plate using 1 µl Lipofectamine LTX (Thermo Fisher Scientific, Cat# 15338100)) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR success was evaluated on a 1 % agarose gel before PCR products were normalised using a SequalPrep Normalisation plate (Thermo Fisher Scientific, MA, USA) following the manufacturer’s instructions with an end concentration of 25 ng per sample (100 μl) ...
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bioRxiv - Immunology 2022Quote: ... A pre-titrated dose of pseudovirus was incubated with eight serial 5-fold dilutions of serum samples (1:20 start dilution) in duplicate in 384-well flat-bottom tissue culture plates (Thermo Fisher, #12-565-344) for 1 hour at 37°C prior to adding 293T/ACE2 cells ...
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bioRxiv - Bioengineering 2022Quote: ... The isolated cells were washed and plated on non-tissue culture treated plates in complete RPMI (10% fetal bovine serum [FBS, Gibco], 1% penicillin/streptomycin [Gibco]) supplemented with 20ng/mL of M-CSF [Miltenyi Biotech]) ...
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bioRxiv - Immunology 2021Quote: ... the virus-serum mixture was added to 1 x104 Vero or Vero E6 cells seeded 3 h before in 96 well plates (Thermo Fisher Scientific, Ulm, Germany). Cells were incubated for 4 days at 37°C in a humidified atmosphere containing 6% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were plated on 6-well plates at 1 × 105 cells/well or onto 4-channel chamber slides (Lab-Tek II chamber slide, Thermo Fisher Scientific, Waltham, MA) at 5 × 104 cells/well and were grown until reaching 70% confluence in serum-free EpiLife medium supplemented with HKGS.
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bioRxiv - Neuroscience 2021Quote: ... N2a cells grown on coverslips were transferred to 35 mm culture plates containing 1 ml of serum and antibiotics free Opti-MEM (Gibco, ThermoFisher Scientific, Waltham, MA, USA). Then ...
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bioRxiv - Cell Biology 2021Quote: ONH astrocytes were seeded at 1 x 104 cells/cm2 on sterilized glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ONH astrocytes were seeded at 1 x 104 cells/cm2 on sterilized glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 24 h ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... The plate was washed for three times and incubated with 100 uL of streptavidin poly-HRP (1:2000 dilution; Thermo Fisher Scientific, Waltham, MA) at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: Both WT and Cx43 KO OCY454 cells following thawing were seeded on rat type I collagencoated plates and grown at 33° C in standard OCY454 media (Supplemental Table 1: αMEM with 10% FBS (Gibco: 26140-079; Lot # 2496659RP) and 2% P/S) ...
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bioRxiv - Genomics 2023Quote: We adapted the neurogenin 2-based neuronal differentiation protocol from (Fernandopulle et al. 2018) using plates and coverslips coated for 1 hour at 37°C with Geltrex (Thermo Fisher Scientific, cat# A1413302) diluted to 1% with DMEM/F12 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was adjusted to 5 µg ml-1 in a total of 100 µl per well diluted antigen and were coated into Pierce™ Nickel Coated Plates (Thermo Fisher Scientific, #15142) at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in PBS at an optical density at 600 nm (OD600nm) of 1 before inoculation into 96-well culture plates (Thermo Fisher Scientific, Waltham, MA) at a final OD600nm of 0.01 in mMRS ...
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bioRxiv - Cancer Biology 2023Quote: ... and were seeded at a density of 1 x 104 cells/well (200 µl culture medium per well) into Nunclon™ Sphera™ 96-well -low attachment plates (Thermo Fisher Scientific) respectively ...
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bioRxiv - Bioengineering 2023Quote: After diluting cells from culture or colonies (see above) 1:10 into PBS+0.1% Tween (total volume=200 μL) in 96-well round-bottom plates (Fisher Scientific cat #07-200-760), samples were measured for cellular composition using a ThermoFisher Attune NxT V6 Flow Cytometer at UW-Madison’s Carbone Cancer Center ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at a density of 1×104 cells/well (200 µl of culture medium per well) into Nunclon™ Sphera™ 96-well low attachment plates (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Levels of Aβ40 in supernatants of HEK or HEKswe cells transfected with different MT5-MMP variants and/or C99 constructs or treated with C3 or DAPT were evaluated by ELISA (#KHB3481, Thermo Fisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2020Quote: ... Commercially available ELISAs for quantitative detection of human total IgG were used according to manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, U.S.A.). All plates were read in a microtiter plate reader at 450 nm and optical density values were used in statistical analyses.
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bioRxiv - Bioengineering 2020Quote: ... The blood samples were then centrifuged at 2000 xg for 10 minutes and serum collected for TNF determination by ELISA (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Recombinant trimeric ectodomain of S protein with six proline mutations (rtS-ecto6P) for enzyme-linked immunosorbent assay (ELISA) was produced using Expi293F cells (Thermo Fisher Scientific). In addition to the mutation at the furin cleavage site ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Blood from all animals of an exposure group was pooled and centrifuged (600 x g) and serum isolated and assessed for immunoglobulin E (IgE) and IgG2a using sandwich ELISA (Invitrogen, Waltham, MA) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed 3 times with TBS-T and phage binding was detected by addition of 20 µl SuperSignal™ ELISA Pico Chemiluminescent Substrate (Thermo-Fisher Scientific) and read on an Envision multilabel plate reader (Perkin-Elmer) ...
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bioRxiv - Bioengineering 2022Quote: ... Dam serum and placental tissue lysates (both prepared as described above) were assayed for IL-6 concentration using an ELISA per manufacturer instructions (Invitrogen, Waltham, MA). Sample absorbance values were compared to a standard curve to calculate IL-6 concentration.
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bioRxiv - Cell Biology 2023Quote: ... ELISA signal was measured using a BioTek Synergy Neo microplate reader one minute after adding SuperSignal ELISA Pico Chemiluminescent substrate (ThermoFisher Scientific, 37069).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... optical densities were determined in 200 µl culture in microtiter plates by spectrophotometry in a plate reader (Spectramax M5, Applied Biosystems, United States) and 20 µl of culture were transferred into 4 ml of fresh MMAB-medium ...
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bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
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bioRxiv - Neuroscience 2020Quote: ... or NAD standard were combined with 50 μl of reagent in a 96-well plate (Nunc™ F96 MicroWell™ White Polystyrene plate, ThermoFisher Scientific). Luminescence was recorded at approx ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...