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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of 1 μM Fluo-4 AM (ThermoFisher Scientific, F14021) in GBO medium was added to each GBO at the ALI surface for at least 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-HuC/D (Elavl3/4) (1:500, Invitrogen A21271) in PBST overnight at 4 °C in a humidified chamber ...
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bioRxiv - Microbiology 2024Quote: ... A 1:4 dilution of Intracellular Fixation Buffer (Invitrogen, Waltham, MA) was added to each chamber ...
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bioRxiv - Microbiology 2023Quote: ... plus DAPI (Thermo Fisher Scientific, 1:1000 in 4% BSA PBS) at room temperature ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: Astrocytes were loaded with Fluo-4 AM (1 mM; Molecular Probes) in culture media for 20 min at 37°C followed by 10 min wash in HBSS (Sigma ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was refreshed every day and cells were passaged every 4-5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 5% (w/v) skimmed milk overnight at 4°C and probed with anti-DsbA (1:1000, Thermo Fisher Scientific) or anti-AP (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were collected by spinning at 1000g for 5 min at 4°C and cell pellets were resuspended in 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were centrifuged at 300 x g for 5 min at 4°C and treated with 1 mL of Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Gibco; cat#A1049201). After 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... as previously described.36,61 Cells were cultivated in FreeStyle F17 expression medium supplemented with 0.1% (v/v) Pluronic F-68 and 4 mM L-glutamine (all from Thermo Fisher Scientific, United States) in shaking flasks at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Restriction-digested shuttle vectors -1-6 (33) and -2-1 (14) for wc-1 and wc-2 respectively were recombined with PCR (Thermo Fisher Scientific, Catalog # F549S) products amplified with primers bearing point mutations of wc-1 or wc-2 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with the respective pcDNA3 constructs and subsequently selected with 2 mg/ml G418 (Geneticin from Thermo Fisher Scientific; 3 mg/ml for 10 days). Surviving cells were expanded in culture medium containing G418.
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Neuroscience 2022Quote: ... 8 μl of 5% hydroxylamine (Thermo Fisher Scientific) was added to quench the reaction ...
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bioRxiv - Developmental Biology 2019Quote: Prab-3::GFP was generated by cutting out Prab-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Developmental Biology 2019Quote: Pelt-3::GFP was generated by cutting out Pelt-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Microbiology 2023Quote: Epimastigotes and trypomastigotes lysates containing 2 x 107 parasites in 20 µL of SDS-PAGE sample buffer containing 5 mM DTT were boiled for 5 min and loaded onto precast Novex Value 4-12% Tris-Glycine gels (Thermo Fisher). The electrophoresis was performed in 50 mM MOPS-50 mM Tris Base ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... mutations were inserted in fragment 8 (Extended Data Table 3) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, cat# A13312) according to the manufacturer’s protocol with the following primers ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sonicated and heated at 72°C for 10 minutes before loading 20 μl/well on NuPAGE™ 3 to 8% Tris-Acetate gels (ThermoFisher, EA03785BOX) alongside high molecular weight markers (ThermoFisher LC5699) ...
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Cell Biology 2021Quote: ... (Horizon Discovery Dharmacon, Lafayette, CO) in DharmaFECT 3 reagent (Horizon Discovery Dharmacon, T-2003) for 6-8 hours in OptiMEMm (Invitrogen, Waltham, MA)65 ...
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bioRxiv - Cancer Biology 2024Quote: ... The resolved samples were run on either NuPAGE 3–8% Tris-acetate or NuPAGE 12% Bis-Tris protein gels (Thermo Fisher Scientific) with the appropriate running buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and 40 μg of protein lysates were resolved on precast 3-8% gels in Tris-acetate buffer and then transferred to nitrocellulose membranes (all from Thermo Fisher Scientific). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved due to 3-8% Tris-acetate SDS-PAGE and transferred onto methanol-activated PVDF membrane with Pierce G2 Fast Blotter (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Cancer Biology 2024Quote: ... + 100 mM beta-mercaptoethanol at 80 °C for 10 min and run on 3-8% Tris-acetate gels (Thermo Fisher Scientific, EA0375PK2). Gels were run at 80V for 3 h and transferred onto a PVDF membrane in Tris-Glycine buler for 90 min at 90V ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 – 50 ug of overall protein containing sample buffer and reducing buffer was loaded onto a 3 – 8% tris-acetate gel (Thermo Fisher Scientific) using tris acetate running buffer (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed 3×5 min in PBST and incubated with secondary antibodies (chicken anti-goat IgG Alexa Fluor 488 (ThermoFisher: A-21467, 1:1000) in blocking buffer for 2 hr at RT ...
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bioRxiv - Neuroscience 2019Quote: ... at 4°C overnight, rinsed in PBS (3 times, 5 min each) and amplified by Alexa Fluor-488 donkey anti-rat antibody (Life Technologies, A21208, 1:600). Sections were mounted with Fluoromount containing DRAQ-5 (1:1000).
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bioRxiv - Neuroscience 2023Quote: P29 mice were anesthetized with isoflurane (1–3%) and 50 nl of the retrograde tracer cholera toxin subunit B (5% wt./vol in PBS, ThermoFisher Scientific, Cat # C-34775) were injected at 10nl/sec into the left SLM (ML ...
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bioRxiv - Biophysics 2021Quote: ... 1 mg/mL in 2:1 chloroform:methanol) is from Invitrogen (USA) and naphthopyrene from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...