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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Following secondary fluorescent antibody incubation (Donkey anti-mouse conjugated Alexa Fluor 568, Donkey anti-Rabbit conjugated Alexa Fluor 488 or 647, Invitrogen), nuclei were stained with DAPI dye (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455 ...
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bioRxiv - Immunology 2024Quote: ... 0.3% Triton X-100 and stained overnight at 37L in blocking buffer containing primary antibody (anti-Krt6, Polyclonal, Proteintech cat. 10590-1-AP, 1:200 dilution; anti-Krt5, Clone 2C2, Invitrogen cat ...
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bioRxiv - Biophysics 2021Quote: ... Cultured cells were stained with mAb 0614 or human chimera MAb 0614-5 as the primary antibody and Alexa Fluor 488– or 647–conjugated anti-rat or human-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... and subjected to immunoprecipitations with goat anti-GFP antibodies (generic goat IgGs as control) or mouse anti-γ-adaptin antibodies (mouse hybridoma C7 antibodies as control) bound to protein G-Sepharose beads (Fisher Scientific). Samples were rotated end-over-end at 4°C for 2 h and washed 2 x in buffer D+ and 1 x in PBS ...
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bioRxiv - Microbiology 2019Quote: ... followed by immunostaining with a rabbit polyclonal MUC2 antibody and goat anti-rabbit Alexa-Fluor 488 antibody (Life Technologies, Paisley, United Kingdom). Slides were imaged using a BX51 upright microscope and a Coolsnap EZ camera (OLYMPUS ...
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bioRxiv - Developmental Biology 2020Quote: The primary antibodies used in this study and their dilutions were the following: chicken anti-GFP antibody (Life Technologies, A10262, 1:500), rabbit anti-DsRed (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibody incubation was for 1 hour at room temperature using HRP conjugated antibodies: anti-mouse IgG at 1:3000(Invitrogen 62–6520) or goat anti-rabbit IgG at 1:3000 (Promega W4011) ...
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bioRxiv - Molecular Biology 2020Quote: ... the primary antibody was rinsed three times with PBS then Alexa Fluor 546-tagged goat anti-rabbit IgG secondary antibody (ThermoFisher, A-11035) was added at a 1:1250 dilution and incubated for 1 hour before a final 3x rinse with PBS.
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bioRxiv - Cancer Biology 2019Quote: ... Then coverslips were inverted onto 100 μL of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse - 1:200, Life Technologies #A11029) and DAPI (DNA ...
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bioRxiv - Immunology 2019Quote: ... Tween 20 and incubated for 2 hr with goat-anti-mouse secondary antibody-Alexa Fluor® 680 conjugate or goat anti-rabbit secondary antibody-Alex Fluor® 800 (0.67 ng/ml; ThermoFisher Scientific). Membranes were washed and developed on the Odyssey Infrared Imaging System (LI-COR) ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS and added with the secondary antibody (Alexa Fluor 488 Conjugated anti-mouse antibody, Life technologies by ThermoFisher Scientific) at 1:250 for overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: GFP was visualized using rabbit polyclonal or mouse monoclonal anti-GFP primary antibodies and Alexa 647-labelled secondary antibodies (Molecular Probes/Invitrogen) directed against rabbit or mouse ...
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bioRxiv - Genomics 2021Quote: ... The secondary antibody used in this study is Alexa Fluor® 647 Donkey Anti-Rabbit IgG (H+L) Antibody (Life Technologies A31573) used at 1:500 dilution.
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bioRxiv - Neuroscience 2020Quote: ... Visualization of immunoreactive signals of hM4Di was performed using an anti-M4 antibody (H-175) and fluorophore-conjugated secondary antibodies (Invitrogen, Carlsbad, USA) as described previously15.
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used here: Alexa Fluor 555-labeled donkey anti rabbit secondary antibody (1:500, Thermo Fisher Scientific, USA), Alexa Fluor 555-labeled donkey anti rat secondary antibody (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... Washed samples were incubated for 1h at RT with the secondary antibody (1:200 Alexa Fluor 647 goat derived anti-rabbit antibody, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with secondary antibody for 3 nights at 4°C with secondary antibodies at 1:500: goat anti-rabbit Alexa Fluor 488 (ThermoFisher #A-11008) and goat anti-mouse Alexa Fluor 647 (ThermoFisher #A-21236) ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of primary antibodies was performed using fluorochrome-conjugated secondary antibodies against goat (Alexa Fluor Plus 647-conjugated, donkey anti-Goat; A32849; Invitrogen; 1:200) and rabbit (Cy3-conjugated ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies used throughout this work include the anti-pan-MHC-I antibody W6/32 (purified antibody from BioXcell, directly conjugated with AlexaFluor 647 via Molecular Probes kit); anti-CD147 (clone HIM6 ...
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bioRxiv - Cancer Biology 2020Quote: ... 65 million cells were stained with the anti-pan-MHC-I antibody W6/32 (purified antibody from BioXcell, directly conjugated with AlexaFluor 647 via Molecular Probes kit), washed 3x in RPMI/FBS-containing staining buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies [Anti GFP rabbit polyclonal antibody (1:500 dilution; #catalog no: A-11122, Thermo Fisher Scientific, Tokyo, Japan)] diluted with the blocking solution overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Microbiology 2019Quote: ... fixed in 4% paraformaldehyde and stained with a primary mouse monoclonal antibody raised against IBDV VP2 [18] and a secondary goat anti-mouse antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific). Wells were marked positive or negative for the presence or absence of virus by immunofluorescence microscopy and the TCID50/mL calculated by the Reed and Muench method [19] ...
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bioRxiv - Neuroscience 2021Quote: Primary antibody was rabbit anti-GFP primary antibody (1:2000; Chemicon, AB 3080) and secondary antibody was donkey anti-rabbit Alexa Fluor 594 (1:2000; Molecular Probes, A21207). In rats in the GFP group ...
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bioRxiv - Immunology 2021Quote: ... The following day macrophages were then washed with 1X PBS to remove unbound Ki67 antibody and incubated with Alexa Fluor-594 goat anti-rabbit secondary antibody (Invitrogen A-11037) at 1:250 dilution for 2 hrs at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... After washing thrice with PBS-0.1% Tween 20 the membrane was probed with primary antibody which is a rabbit monoclonal anti human lamin A+C antibody (Thermo Scientific, USA) and Stabilized Peroxidase Conjugated secondary Goat Anti-Rabbit (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Neuroscience 2020Quote: ... The cells were then treated for 60-90 min with a secondary antibody solution containing goat anti-guinea-pig IgG (H+L) antibody conjugated with Alexa Fluor 594 (A-11076; Thermo Fisher Scientific) (1,000× dilution) ...
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bioRxiv - Systems Biology 2021Quote: ... the cells were washed three times with 100 μL of PBS manually and stained with diluted secondary antibody solution (Alexa-Fluor 568-conjugated goat anti-rabbit IgG antibody) with DAPI (ThermoFisher #A-11011). After incubating for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... The rat anti-Sls antibody (anti-Kettin, MAC155/Klg16, Babraham Bioscience Technologies) was diluted 1:1000 and visualised with fluorescently labelled secondary antibodies (ThermoFisher, Molecular Probes). Actin was stained with phalloidin-rhodamine or phalloidin-Alexa488 (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibody was then labeled for 1h at room temperature using Alexa488 goat anti-mouse IgG secondary antibody (Invitrogen, OR, USA) at a 1:750 dilution in PBS ...
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... heart tissue sections were incubated at room temperature for 2 hr with secondary antibodies: goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 488 conjugate 1:500 (A11001, Thermo Fisher Scientific); goat anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2022Quote: ... REX1 antibody DA136 (3rd bleed) was diluted at 1:1000 and visualized using Donkey anti-Sheep HRP secondary antibody (Thermo Fisher Scientific). Immunoblots were imaged using enhanced chemiluminescence on a BioRad ChemiDoc imager (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting clones were screened via immunoblotting with gene-specific antibodies anti-NINL rabbit polyclonal antibody (Thermo Fisher Scientific, Waltham, MA,USA) and anti-NIN mouse monoclonal antibody (LSBio ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated at room temperature for 1 hour in secondary antibody solution which contained Alexa 488-conjugated anti-mouse antibody (1:200; Thermo Fisher Scientific), rhodamine phalloidin (1:50 ...
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bioRxiv - Cell Biology 2019Quote: ... Caveolin-1 rabbit polyclonal antibody (# PA1-064) and anti-pFAK (Y397) rabbit monoclonal antibody (# 44624) obtained from Thermo Fisher (Grand Island, NY).
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained with M2 FLAG primary antibody (SigmaAldrich, F1804) and AlexFluor 594-conjugated anti-mouse goat antibody (ThermoFisher, A-11005) using the same conditions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with biotin-conjugated anti-PE antibody together with antibodies for surface markers for flow cytometric analysis using AttuneNxT (Thermo Fisher Scientific) and FlowJo 10.5.2 (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were used at the same concentration as their respective primary antibodies and included donkey anti-rabbit 568 (ThermoFisher, cat# A10042), donkey anti-chicken 647 (Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... They were then washed with PBS three times and incubated with secondary antibody: either Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) antibody (Thermofisher; A-11008) or Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody was then detected by an Alexa Fluor™ 488-conjugated goat anti-mouse secondary antibody (Invitrogen, Cat# A-11001), which was diluted 1:500 in blocking solution (PBS containing 1% FCS [Bioconcept ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4 °C with the primary antibody rabbit anti-CaMKIIα polyclonal antibody (1:1000, Invitrogen PA5-84083), mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were immunostained with goat anti-calbindin (1:300) as the primary antibody and anti-goat Alexa Fluor 488 (1:200) as the secondary antibody (Invitrogen, Eugene, OR). Sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Cells from infectivity and replications assays were incubated 1 hour at room temperature with anti-cytosolic tryparedoxin peroxidase (cTXNPx) primary antibody (Pineyro et al., 2008) and 30 minutes with Alexa Fluor 488 anti-rabbit IgG secondary antibody (Invitrogen, Eugene OR). Parasites from the epimastigogenesis experiment were attached to poly-L-lysine-coated slides and treated as monolayers ...
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bioRxiv - Microbiology 2022Quote: ... HBLAK cells grown on membranes were incubated with the following primary antibodies: rabbit anti-TLR-2 polyclonal antibody (10 µg/ml, PA5-20020, Thermo Fisher Scientific), rabbit anti-TLR-4 polyclonal antibody (5 µg/ml ...