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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... for detection in QuantStudio 6 Pro cycler (Applied Biosystems Carlsbad, CA). Quantification of each transcript was normalized to the mouse 18S reference gene following the 2-ΔΔCt method ((Livak & Schmittgen ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was eluted 6 times in ultrapure water (Thermofisher, 10977) for maximum recovery ...
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bioRxiv - Neuroscience 2024Quote: ... were coated with IL-6 capture antibody (ThermoFisher Scientific, MP5-20F3) and left overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... and analysed using the Quant Studio 6 Flex system (Applied Biosystems). The real-time qPCR conditions were one hold at (95 °C ...
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bioRxiv - Physiology 2024Quote: ... on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with specific primers for each gene ...
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bioRxiv - Neuroscience 2024Quote: ... or Quant Studio 6 Pro Real-Time PCR System (Applied Biosystems). TaqMan assay details are listed in Tab ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.30 M di-benzo-18-crown-6-ether (Thermo Scientific) following published protocols66,67,79,80,110 ...
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bioRxiv - Cancer Biology 2024Quote: ... The desired DNA product was purified with 6% TBE gel (Invitrogen) and samples were sequenced on an Illumina HiSeq2000 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown for 6 passages in DMEM-HAM’s F12 (Gibco) supplemented with 5% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Microbiology 2024Quote: ... 6-5 cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FetalPlex (Gemini Bio-Products) ...
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bioRxiv - Immunology 2024Quote: ... and real-time PCR system (Applied Biosystems 7500 QuantStudio 6 Pro). The Ct value of target genes obtained from each sample was normalized to housekeeping gene GAPDH and relative gene expression was quantified using 2-ΔΔct values.
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bioRxiv - Molecular Biology 2024Quote: ... and eluted in 6 μl nuclease-free water (Ambion, cat# AM9937).
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bioRxiv - Biophysics 2021Quote: The cDNA segment encoding the C1B domain of protein kinase C (PKC) δ isoenzyme from Rattus norvegicus (amino acids 229-281) was sub-cloned into pET SUMO expression vector (Invitrogen). The His6-SUMO-C1Bδ fusion protein was expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in Dulbecco’s modified Eagle’s minimum essential medium supplemented with 20% heat-inactivated fetal bovine serum and 1% non-essential amino acids (Gibco, Waltham, MA), and cultured in 10% CO2/90% air ...
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bioRxiv - Genetics 2021Quote: ... then resuspended in differentiation medium (20% KnockOut Serum Replacement and MEM Non-Essential Amino Acids in DMEM-F12, all Thermo Fisher) supplemented with a small molecule cocktail (1 µM dorsomorphin ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Biochemistry 2019Quote: ... and 10∼10,000 μM concentration of each amino acid in 20 μL of assay buffer and loaded to a MicroAmpR Fast Optical 48-Well Reaction Plate (Applied Biosystems). Fluorescent Intensity was measured by the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... Neural induction was initiated in KSR medium (Knockout-DMEM, 15% knockout-serum replacement, 100 µM β-mercaptoethanol, 1% nonessential amino acids 1%, Glutamax (ThermoFisher)) by dual SMAD inhibition (SB431542 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS and cultured with amino acid free DMEM (GENAXXON bioscience) containing 1% penicillin-streptomycin and dialyzed FBS (Thermo Scientific) if indicated ...
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bioRxiv - Cell Biology 2019Quote: ... Both DifcoTM YPD broth and DifcoTM Yeast Nitrogen Base (YNB) w/o Amino Acids and Ammonium Sulfate were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... the oligonucleotide sequence 5’-GGA CAA TTA CTA TTT ACA ATT ACA ATG NNN NNN NNN NNN NNN NNN NNN NNN NNN NNN AAA GGC GGC AGC GGC GGC AGC CAT CAC CAC CAT CAC CAT ATG GGA ATG-3’ encoding the 10-mer (MX1X2X3X4X5X6X7X8X9X10K) library (where X is one of the 20 natural amino acids) was PCR-amplified with Phusion DNA polymerase (Fisher Scientific), using the forward primer 5’-GCA AAT TTC TAA TAC GAC TCA CTA TAG GGA CAA TTA CTA TTT ACA ATT AC-3’ and the reverse primer 5’-ATA GCC GGT GCC AGA TCC AGA CAT TCC CAT ATG GTG ATG GT-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... the medium was switched to standard human embryonic stem cell (hESC) medium (High glucose DMEM with 20% Knockout Serum Replacement, non-essential amino acids, Glutamax+ penstrep (Life Technologies), 2-mercaptoethanol (100µM ...
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bioRxiv - Neuroscience 2019Quote: ... non-essential amino acids solution (1/100) and 100 units/ml penicillin/streptomycin (all supplements were from Invitrogen, Paisley, Scotland, UK) before replacing with fresh medium ...
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bioRxiv - Microbiology 2020Quote: ... Homogenate was resuspended with 6 ml per brain of prewarmed complete media (DMEM [Corning]; 10% FBS; 1% Nonessential Amino Acids Mixture 100x, [Biowhittaker Reagents Lonza],1% GlutaMAX supplement 100x [Thermo Fisher Scientific] ...
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bioRxiv - Systems Biology 2020Quote: RPE differentiation medium (10% KSR): 0.094 mM MEM Non-Essential Amino Acids Solution (NEAA) (#11140050, Thermo Fisher Scientific Inc., MA, USA), 0.94 mM sodium pyruvate (#S8636 ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal bovine serum, 0.1 nM non-essential amino acids (Wisent, Montreal, Canada) and 100 ug/ml Pen/Strep (Invitrogen, Burlingtion, Canada). miR-122 knockout Huh-7.5 cells were a kind gift from Dr Matthew Evans ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg of plasmid encoding SARS-CoV2 spike (D614) with C-terminal 18 amino acid deletion using Lipofectamine LTX Plus reagent (Invitrogen, USA) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was replaced with EB medium (DMEM with 15% FBS, 1% Non-Essential Amino Acids and 0.1 mM β-mercaptoethanol, Gibco 31350010). After 4–7 days ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells (ATCC®, C1008) were grown in MEM/EBSS with L-Glutamine (Hyclone) supplemented with 1X non-essential amino acids (Gibco), 1 mM sodium pyruvate (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... Peptide conjugation was verified by creating standard curves for SILY and LXW7 using concentration dependent 280 nm absorbance of aromatic amino acids and extrapolating absorbances of synthesized molecules using readings taken on a NanoDrop UV-Vis spectrophotometer (Thermo Fisher). The structures of LXW7-DS-SILY and the reaction scheme of making LXW7-DS-SILY was described in a previous study [22].
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bioRxiv - Developmental Biology 2022Quote: Hepatocyte differentiation medium was made using complete HepatoZYME (Life Technologies 17705-021) (i.e. basal HepatoZYME supplemented with nonessential amino acids (ThermoFisher 11140050), chemically defined lipid concentrate (ThermoFisher 11905031) ...
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bioRxiv - Microbiology 2022Quote: ... Amino acids were extracted on ice-cold lysis buffer [5:3:2 ratio of methanol-acetonitrile-water (Fisher Scientific, Pittsburgh, PA)] containing 3 µM of amino acid standards [Cambridge Isotope Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A codon-optimized gene encoding for SARS-CoV-2 (331 to 528 amino acids, QIS60558.1) was expressed in Expi293 cells (Thermo Fisher Scientific) with human serum albumin secretion signal sequence and fusion tags (6xHistidine tag ...
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bioRxiv - Immunology 2022Quote: ... followed by an antibody cocktail in complete RPMI (cR10, RPMI-1640 media supplemented with 10% FBS, 1x non-essential amino acids (Gibco #11140050), and 10mM Hepes (Gibco #15630080) ...
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bioRxiv - Microbiology 2022Quote: ... NNK was introduced into each codon of the interest (D18-S342, H1 numbering from the first amino acid residue) by overlapping PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) to generate full-length HA amplicons ...
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bioRxiv - Cell Biology 2019Quote: ... the serum was collected then affinity purified using His-CSAG1 linked to the amino link plus coupling resins (Thermo scientific, #20501) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... An HA tagged version of murine SDC$ was also synthesized with the coding sequence being inserted in between the same amino acids (GeneArt, Life Technologies). These cDNAs were ligated into the BamHI and XhoI sites of the lentiviral vector plentiSFFV-MCS using conventional procedures ...
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bioRxiv - Systems Biology 2020Quote: RPE differentiation medium (20% KSR): 0.10 mM MEM Non-Essential Amino Acids Solution (NEAA) (#11140050, Thermo Fisher Scientific Inc., MA, USA), 1.0 mM Sodium pyruvate (#S8636 ...
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bioRxiv - Systems Biology 2020Quote: RPE differentiation medium (15% KSR): 0.10 mM MEM Non-Essential Amino Acids Solution (NEAA) (#11140050, Thermo Fisher Scientific Inc., MA, USA), 0.99 mM sodium pyruvate (#S8636 ...
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bioRxiv - Cell Biology 2020Quote: C2C12 myoblasts were transfected with a plasmid containing the N-terminal 88 amino acids of human cyclin B1 fused to EGFP using Lipofectamine 3000 (Invitrogen, L3000001) with the P3000 reagent according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Oligonucleotides containing an amino-modified dT were labelled with either Alexa Fluor 488 or Alexa Fluor 594 NHS ester (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... SP140ΔPHD) or Bromodomain (amino acids 796-829, SP140ΔBromo) were generated by overlap-extension PCR using Phusion HF polymerase (Thermo Fisher Scientific). HA-Aire plasmid was obtained from Sun Hur (Boston Children’s Hospital ...
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bioRxiv - Immunology 2021Quote: ... MN908947) C-terminally truncated by 19 amino acids was cloned into a derivative of the pCDNA3.1 expression vector (Thermo Fisher Scientific). Substitutions and deletions in the Spike protein gene open reading frame (shown in Figure 1 ...
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bioRxiv - Cell Biology 2020Quote: Organoid basal media (OBM) consists of Advanced DMEM/F12 with non-essential amino acids and sodium pyruvate (Fisher Scientific #12634-028) containing 1x N-2 (Fisher Scientific #17502-048) ...
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bioRxiv - Plant Biology 2022Quote: ... Untargeted profiling of amino acids and other cationic metabolites was performed using the Vanquish Focused ultra-high-pressure liquid chromatography (UHPLC) system (Thermo Scientific) coupled to a QExactive Plus mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were cultured in the same medium as HEK 293 cells but supplemented with 1x non-essential amino acids (Gibco, 11140050). All cells were kept at 37°C in a 5% CO2 humidified atmosphere.
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bioRxiv - Neuroscience 2022Quote: ... 1% penicillin-streptomycin and 1% non-essential amino acids) as described previously (37) and transduced using CytoTune™ –iPS 2.0 Sendai Reprogramming kit (ThermoFisher Scientific) according to manufacturer’s instructions ...