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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate; Thermo Scientific, Rockford, IL). Blot band densities were obtained using the ChemiDoc imaging system (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were probed with the appropriate primary antibodies followed by goat anti-rabbit IgG or goat anti-mouse IgG conjugated to horseradish peroxidase and visualized using enhanced chemiluminescence substrate (SuperSignal™ West Dura Extended Duration Substrate #34076, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The membranes incubated with HRP conjugated secondary antibody were further developed using enhanced chemiluminescence (SuperSignal™ West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific). All membranes were imaged on a Bio-Rad ChemiDoc XRS+ System.
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed again three times in PBS-T and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Carlsbad, CA). The specifications ...
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and peroxidase activity was detected using chemiluminescent reagent (SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher, Cat#34580). Images of developed strips were acquired on Molecular Imager® Gel Doc™ XR System (BioRad ...
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bioRxiv - Immunology 2022Quote: ... Membranes were rinsed again three times in PBS-T and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Carlsbad, CA). The specifications ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotting was performed as described earlier 32 and the HRP-labelled secondary antibodies were developed with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific #34580) or with SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific #34095 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Chemiluminescence was detected through the Bio-Rad ChemiDoc MP Imaging system using the SuperSignal West Femto Maximum Sensitivity Substrate (Cat# 34096, Thermo Scientific, Waltham, MA). Bio-Rad Image Lab software was used for densitometric analyses ...
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bioRxiv - Cell Biology 2021Quote: ... SuperSignal West Pico PLUS Chemiluminescent Substrate was used for detection and blots were visualized with an iBright Western Blot (Thermo Fisher, Waltham, MA) imaging system.
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bioRxiv - Developmental Biology 2020Quote: ... After three 15 minutes washes with PBST at room temperature signal was detected using the SuperSignal™ West Femto kit (Thermo Scientific, # 34095). Images were processed and quantified using ImageJ program.
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed three times in 1X TBST for 5 minutes and developed using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... membranes were washed three times for 10 min at RT with 1 x TBS-T and developed using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, 34075) according to the manufacturer’s instructions on the BioRad ChemiDoc XRS+ imaging system.
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bioRxiv - Physiology 2022Quote: ... All primary antibodies were revealed with HRP-conjugated goat secondary antibodies using the Bio-Rad ChemiDoc MP and detected using SuperSignal™ West Pico or Femto Chemiluminescence Substrate (Thermo Fisher Scientific). Protein densitometry was analyzed by Image Lab software (6.0).
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bioRxiv - Cell Biology 2024Quote: ... and membranes were washed 3×5 minutes in 1X TBS-T before being developed using Pierce SuperSignal West Pico PLUS (Thermo Scientific, cat: 34580) (β-catenin ...
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bioRxiv - Genetics 2023Quote: ... Membranes were washed three times with PBST for 10 min and the signal was developed using the SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, PI-34080) and detected using an Amersham Imager 600 (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed with 1x TBST (4 x 10 min) and processed using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific PI34080).
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A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2 deficient pancreatic cancerbioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated in primary antibody overnight with rocking at 4°C followed by incubation in appropriate secondary antibodies conjugated to horseradish peroxidase (1:5000) for 1 hr and detection by Super Signal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577). Primary antibodies used were ...
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bioRxiv - Plant Biology 2023Quote: ... Chemiluminescence was detected with ECL Prime Western Blotting Detection Reagent (Cytiva, GERPN2232) and SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, 34094).
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times and the signal was detected using the chemiluminescent substrate SuperSignal West Pico Plus (Thermo Scientific, REF 34580) and the imaging system Vilber Fusion FX7 Edge 17.10 SN ...
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bioRxiv - Systems Biology 2023Quote: Labeled blots were washed 4 x 5 min each then incubated in SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific™ #34577) at room temperature for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). Proteins were then quantified with Image Studio Version 5.2.4 (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed 3 times for 10 minutes in TBST and immunoreactive bands were detected with ECL solution SuperSignal™ West (Thermo Scientific; #34577) after 40 seconds of exposure in a FluorChemE system (ProteinSimple California ...
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bioRxiv - Molecular Biology 2023Quote: ... and developed with a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate (SuperSignal West Dura Extended Duration Substrate, Thermo Scientific™ #34076)
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bioRxiv - Cancer Biology 2023Quote: ... The blots were developed using an enhanced chemiluminescence western blotting detection system (SuperSignal West Dura Extended Duration Substrate, Thermo Scientific, Waltham, MA, USA). Images were acquired with a digital imager (Azure Biosystems c600 ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% of skim milk, 1x TBS-T, and detected by ECL (Cytiva, #RPN2106) alone or supplied with 10% of SuperSignal West Femto (Thermo Fisher Scientific, #34095) when required.
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bioRxiv - Biochemistry 2024Quote: ... Detection of the immunoreactive bands was performed using the chemiluminescent reagent SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific; Waltham, MA, USA). Chemiluminescence images were acquired by Azure 600 Imaging System (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were then transferred to PVDF membranes at 100 V for 2 hours and blotted with the indicated primary antibodies (Table 4. Secondary anti-mouse HRP antibody and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific 34096) was used to visualize bands with a Bio-Rad ChemiDoc Imaging System.
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bioRxiv - Physiology 2022Quote: ... Protein abundance was normalized to total protein using Pierce™ Reversible Protein Stain Kit for PVDF Membranes (cat 24585; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Plant Biology 2019Quote: ... 2002) containing the 35S Cauliflower Mosaic virus promoter and eGFP gene using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA). A single colony was used to amplify the [Gene-pK7FW2/pK7WGF2] plasmid and it was sequenced before transforming Agrobacterium (strain GV3101 ...
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and Mayaro virus (MAYV, TRVL 15537) were propagated in Aedes albopictus C6/36 cells grown in Minimum Essential Medium (MEM, Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 (SARS-CoV-2/CANADA/VIDO 01/2020 ...
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bioRxiv - Neuroscience 2021Quote: Images of whole brain slices for analysis of virus expression were obtained from a multichannel fluorescence microscope (EVOS FL Auto Imaging System, Thermo Fisher Scientific, Waltham, MA) with an automatic objective at 10X magnification ...
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bioRxiv - Microbiology 2021Quote: ... cDNA were synthesized from 100 pg of RNA and quantified using the TaqMan® Fast Virus 1-Step Master Mix (Thermofisher, Waltham, Massachusetts, USA) following manufacter’s instructions with appropriate oligonucleotides and Taqman probes (Table S1) ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was collected into 500 μL fractions and the virus containing fractions were detected using NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts, US). The virus containing sucrose fractions were diluted at least 1:5 in 2 mM MgCl2-PBS and the virus was pelleted by centrifugation (70Ti rotor ...
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bioRxiv - Microbiology 2023Quote: Cell culture supernatants or P1 virus cultures were mixed with a five-fold excess of TRIzol™ LS Reagent (Thermo Fisher Scientific, Waltham, MA). Viral RNAs were extracted by Direct-Zol™ RNA miniprep Plus Kit (ZYMO RESEARCH ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was subjected to one-step RTqPCR analysis as described using primers specific for the viral genome encoding for the RNA-dependent RNA polymerase (RdRp)23 and for E24 genes with TaqMan fast virus 1-step master mix (ThermoFisher Scientific, catalogue number 4444432) using AriaMx instrumentation (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was directly subjected to one-step RT-qPCR analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA) and Bio-Rad CFX instrumentation (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies, Buenos Aires, Argentina) in a 20 µL reaction mixture ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA in the protein samples was sheared by sonication and the amount of protein was estimated using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated on 4–20% Mini-PROTEAN® TGX™ Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™, PI23227). The desirable amount of the protein lysate (15 – 30 μg ...
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bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014, respectively) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...