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Citations for Thermo Fisher :
4501 - 4550 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting plasmid was transfected into the wild-type human iPSCs using Lipofectamine (CMAX00015, ThermoFisher Scientific). Transfected iPSC clones were selected against 5 μg/ml Puromycin (A1113802 ...
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bioRxiv - Microbiology 2023Quote: ... followed by an incubation with mouse anti-human E-cad cytoplasmic domain mAb (4A2C7, Life Technologies). In some experiments ...
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bioRxiv - Immunology 2023Quote: ... Human primary CD4+ T cells were cultured overnight in RPMI-10 media (RPMI 1640, 11875119; Gibco), penicillin-streptomycin (100 U/ml ...
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bioRxiv - Microbiology 2022Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) and the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
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bioRxiv - Molecular Biology 2023Quote: ... a single siRNA targeting the human TP53 gene was synthesized and purchased from Ambion (Life Technologies). Briefly ...
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bioRxiv - Biochemistry 2023Quote: Human PSA for crystallization was expressed in insect cells using the BAC-TO-BAC system (Invitrogen) as described previously [34 ...
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bioRxiv - Bioengineering 2023Quote: Human Colon Adenocarcinoma cell line COLO 205 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The HL-60 human leukemia cells (ATCC) were cultured in IMDM (Iscove’s Modified Dulbecco’s medium, Invitrogen) supplemented with 10% FBS and antibiotics ...
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bioRxiv - Developmental Biology 2023Quote: ... and anti-FREM2 human antibody with 77% immunogen identity with zebrafish Frem2a/b (Invitrogen PA5-55986) were used ...
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bioRxiv - Immunology 2023Quote: ... and the CH1 exon of human IGHG1 were codon optimized using the GeneOptimizer tool (Thermo Fisher) and synthesized as gBlocks (IDT) ...
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bioRxiv - Cell Biology 2023Quote: ... WBCs were then stained with PE anti-human CD45 antibody (Thermo Fisher Scientific, 12-0459-42) and APC anti-human CD3 antibody (BioLegend ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Bioengineering 2023Quote: ... Prior to electroporation isolated human T cells were activated for 48h with CD3/CD28 Dynabeads (Thermofisher) at a cell to beads ratio 1:1 together with the recombinant human cytokines IL-2 (150U/ml) ...
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bioRxiv - Genetics 2023Quote: ... Human pluripotent stem cell lines were cultured on geltrex-coated plates in StemFlex Medium (A3349401, Gibco) to 80-90% confluence and passaged with TrypLE (12605036 ...
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bioRxiv - Cell Biology 2023Quote: ... Lightly and darkly pigmenting neonatal primary human melanocytes (LP and DP respectively) were procured from Invitrogen, Waltham ...
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bioRxiv - Cell Biology 2023Quote: ... The complete human ATP8B2 cDNA (NM_001370597) was inserted into pENTR3C (Thermo Fisher Scientific, Waltham, MA, USA) and transferred to expression vectors using the Gateway system (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: mitochondrial status within human heart organoids was visualized using Mitotracker Deep Red FM (Thermo Fisher Scientific). Mitotracker was prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Human Colon Adenocarcinoma cell line COLO 205 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Biophysics 2023Quote: Human neuroblastoma cell line (SK-N-BE) stored at -80°C in freezing medium (RPMI Gibco® supplemented with 20% FBS Gibco and 10% DMSO) ...
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bioRxiv - Cell Biology 2023Quote: HepG2 human hepatocytes were purchased from ATCC and cultured with 5.5mM low glucose DMEM medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ng/mL human basic FGF2 (Miltenyi Biotech, 130-093-840) and kanamycin (Gibco, 15160-054). Culture medium was exchanged every 2 days ...
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bioRxiv - Neuroscience 2023Quote: ELISA was performed on total and SEC fractions using Tau (Total) Human ELISA Kit (KHB0041, Invitrogen) by following the directions provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: The human hepatocarcinoma cell lines Huh7 and Huh7.5.1 were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% heat inactivated fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were obtained from ECACC/PHE and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Microbiology 2023Quote: ... and both bacteria-specific and human-specific rRNA probes as per the manufacturer’s instructions (Life Technologies). Ribo-depleted RNA was concentrated using the RNA Clean & Concentrate Kit-5 (ZYMO) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T human embryonic kidney cells and Vero African Green monkey cells were maintained in DMEM (Gibco) containing 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: H1HeLa (Human cervical adenocarcinoma cells) were maintained in Dulbecco′s Modified Eagle′s Medium (DMEM; Gibco) supplemented with heat-inactivated 10% fetal calf serum (FCS ...
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bioRxiv - Genomics 2023Quote: Immortalised human mammary luminal epithelial cells (HMLE) were cultured in HUMEC media (Gibco, cat# 12753-018). Stable mesenchymal HMLE cells (mesHMLE ...
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bioRxiv - Bioengineering 2023Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-human IgM and IgA (Invitrogen, A18835 and A18781, 1:5,000), rabbit anti-human IgG antibody (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: DNA encoding the ECR of human ADGRB2/BAI2 (aa 1-921) was synthesized by Thermo Fisher GeneArt ...
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bioRxiv - Bioengineering 2023Quote: Mouse cells and human cells were dissociated with 0.25% Trypsin-EDTA (cat. 25200056, Thermo Fisher Scientific) and TrypLE (cat ...
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bioRxiv - Bioengineering 2022Quote: Human embryonic kidney 293T (HEK) cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, 41966-029) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2023Quote: Human HEK293T (referred to as 293T) were purchased from ATCC and cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: H2122 cells (human lung cells, ATCC CRL-5985) were grown in RPMI-1640 medium (Gibco #A10491) with 10% FCS (Hyclone #SH30084.03n ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Human monocyte-derived macrophages (HMDMs) were cultured in media consisting of RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/ml human basic FGF (R&D) on either CELLstart Substrate (A1014201, Gibco) or Vitronectin (A31804 ...
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bioRxiv - Cancer Biology 2023Quote: ... H69 and H524 human SCLC cell lines were maintained in culture in RPMI 1640 (Life Technologies) supplemented in 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Human mammary epithelial cells (MCF10A, ATCC) were maintained in growth medium consisting of DMEM/F12 (Gibco), 5% horse serum (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were obtained from the ATCC and grown in DMEM (Gibco) containing 10% fetal bovine serum (Seradigm) ...
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bioRxiv - Developmental Biology 2023Quote: Primary adult human keratinocytes (ATCC) were cultured on PO/Fibronectin/Laminin coated in Keratinocyte SFM (Gibco). At the start of co-culture ...
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bioRxiv - Developmental Biology 2023Quote: Human ESCs from H9 line (WiCell) were routinely cultured in StemFlex medium (Thermo Fisher Scientific A3349401) on 0.5-mg/cm2 laminin-coated plates (Thermo Fisher Scientific A29249) ...
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bioRxiv - Systems Biology 2022Quote: ... Normal human BJ fibroblasts were obtained from ATCC (CRL-2522) and cultured in Knockout DMEM (Gibco) supplemented with 15% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500); Donkey Anti-Mouse IgG H&L Alexa Fluor 647 conjugated (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500). Data acquisition was performed with FACSVerse™ (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with fluorescent conjugated secondary anti-human IgG antibody (Thermo Fisher, #A21090 and #A-11013) and DNA dye DAPI ...