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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... lacking a stopcodon were generated in a universal entry vector (pDONR221) for the use with the Gateway™ recombination system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Real-time PCR was conducted using 1× FAM labelled Taqman® Gene MGB probes with 1-5 ng of cDNA template using 1× Taqman Universal PCR Master Mix (Applied Biosystems). Reactions and analysis were performed in a CFX-Connect Real-Time System with the following cycle conditions ...
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bioRxiv - Molecular Biology 2019Quote: cDNA was diluted with double-distilled H2O and mixed with TaqMan Universal PCR Master Mix (Cat # 4304437, Life Technologies, Waltham, MA, USA) to a final concentration of 1 mg/ml and assayed for GPCR expression using TaqMan GPCR arrays (Cat # 4367785 ...
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bioRxiv - Neuroscience 2021Quote: ... One third of the reverse transcription mix was subjected to PCR amplification with TaqMan® Universal PCR Master Mix No AmpErase (Applied Biosystems) and the respective TaqMan® reagents for target miRNA ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCRs were performed with primers and either FAM-TAMRA-labeled TaqMan probes combined with TaqMan Universal PCR Master Mix or SYBR Green Master Mix without probes (all from Thermo Fisher Scientific), as indicated in Table 2 ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... The number of viral genome copies for the strain specified in the experiment was spiked into 50 ng of Universal Human Reference RNA (ThermoFisher Scientific #QS0639) to the final volume of 11 μl ...
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bioRxiv - Plant Biology 2022Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Relative transcript abundances of the selected genes were calculated with the ddCt method (52 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 ng of cDNA (based on RNA concentrations) was transferred to each well containing TaqMan Universal Master Mix II (Thermo Fisher Scientific) and a target specific TaqMan assay (assays reported in Supplementary methods ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cDNA was then used for real-time qPCR with the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02) using the Quant Studio™ 6 Flex Real-Time PCR system (Thermo Fisher). The mRNA levels of GAPDH or actin were used as an internal control to normalize the mRNA levels of genes of interest ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression of the indicated target genes was determined by real-time PCR using the TaqMan® Fast universal PCR master mix and 7500 Fast Real-Time PCR System from Applied Biosystems. Based on Ct values ...
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bioRxiv - Neuroscience 2024Quote: ... Nine volumes of brain homogenate were mixed with one volume of 10X detergent buffer [5% (w/v) sodium deoxycholate and 5% (v/v) Nonidet P-40 in PBS] containing Pierce Universal Nuclease (ThermoFisher Scientific #88701) and Halt Phosphatase Inhibitor (ThermoFisher Scientific #78420) ...
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bioRxiv - Cell Biology 2024Quote: ... The qPCR was conducted using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme Biotech, China) in the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, USA). The 2−△△CT method was used to calculate the relative expression (Livak & Schmittgen ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed using 4.5µL of 5-fold diluted RT product and 5.5µL of reaction mix (composed of 5µL of TaqMan Universal Master Mix (Applied Biosystems, Life Technologies, Carlsbad, USA, #4440047) with 0.5µL of the appropriate assay for each miRNA (miR-139-5p ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR reaction was set up with 1 μl of diluted complementary DNA and 20X TaqMan probes and TaqMan Universal PCR Master Mix (Life Technologies, 4324018) in a 15 μl total volume ...
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bioRxiv - Biochemistry 2024Quote: ... 1% NP-40 and 5% Glycerol) supplemented with 125 Units/mL of universal nuclease (Pierce) and 1x Halt™ Protease Inhibitor Cocktail (Thermo Scientific). Lysates were centrifuged at 14,000 rpm for 15 min at 4° C and the supernatant was normalized by A280 with cell lysate dilution buffer (25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: Primary spinal microglia grown on poly D-lysine coated glass-bottom dishes were loaded with Fura-2 by incubating cells in 2 uM Fura-2-AM (Invitrogen) in growth medium for 30 min at 37℃ ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of Superscript IV enzyme, 2 µL of 100 mM DTT and 2 µL of RNAseOut rnase inhibitor, Invitrogen) were added to each well ...
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bioRxiv - Bioengineering 2022Quote: ... formalin-fixed fibrotic capsule tissue samples (n=2 per group) and subcutaneous tissue samples (n=2) were placed in a solution of 2 mg/ml FITC (Invitrogen) in DMEM/F12 media (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME) (All from Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Nuclear factor erythroid 2-related factor 2 (NRF2) (ThermoFisher Scientific, Carlsbad, CA), samples diluted 1:2 ...
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Neuroscience 2020Quote: ... 50mg/ml streptomycin and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2 μL forward primer and 2 μL reverse primer (Invitrogen; Table 1), 14.2 μL water (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiomyocytes were loaded with Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen) as described previously.28 After Fura-2 loading ...
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bioRxiv - Immunology 2022Quote: ... + 200 IU/mL IL-2 (NCI) + 0.08 mM 2-Mercaptoethanol (Gibco, 21985023) (NK-92 Media ...
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bioRxiv - Immunology 2022Quote: ... 20 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... 2 mM L-glutamine and 53 μM 2-mercaptoethanol (all from Gibco). B cells were isolated using EasySep human naïve B cell Enrichment Kit (Stemcell Cat.# 19254 ...
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bioRxiv - Immunology 2019Quote: ... 2 mM L-glutamine and 53 μM 2-mercaptoethanol (all from Gibco) and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 2 μM fura-2 AM (Invitrogen, Waltham, MA) for 25 minutes at 35°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2 μl of 2 × Superscript III enzyme blend (Life Technologies, 11754). All the reactions were incubated at 25 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 2 min incubation in 2-propanol (Thermo Fisher Scientific, USA) and equilibration with 25 µl of solvent A (0.1% FA in H2O ...
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bioRxiv - Immunology 2024Quote: ... or anti-MD-2 (2 µg/ml) (Thermo Fisher Scientific, PA5-20058) for 3 h at RT ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell extracts obtained from 2×105 cells with an NE-PER Nuclear and Cytoplasmic Extraction kit (Thermo Fisher Scientific, 78833) were loaded into a 12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Immunology 2021Quote: Cells exposed to SARS-CoV-2 pseudovirus were lysed and mRNA was isolated with the mRNA Catcher™ PLUS Purification Kit (ThermoFisher). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was removed from 20 μg of RNA with 2 reactions of the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg linearized plasmid DNA was used in a 30 µl reaction with the mMessage mMachine SP6 Transcription Kit (Invitrogen: AM1340). RNA integrity was examined on a 1% agarose gel ...
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bioRxiv - Molecular Biology 2020Quote: ... was performed as follows: 2 μg of messenger RNA (mRNA) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Immunology 2022Quote: ... the SARS-CoV-2-TCR mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Scientitic) following the manufacturer’s protocols ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Developmental Biology 2019Quote: PAT-PCR for the FBF target cyb-2.1 and control tbb-2 was performed using a Poly(A) Tail-Length Assay Kit (Thermo Fisher Scientific). RNA templates from fbf-1(lf) ...
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bioRxiv - Neuroscience 2019Quote: The study of proliferation and differentiation of neural stem cells in the dentate gyrus was performed by incorporation of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Imaging Kit; Molecular Probes) to allow the analysis of proliferation and differentiation ...
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bioRxiv - Molecular Biology 2019Quote: ... LB was added to the sorted cells and they were grown at 37°C for 2 hours prior to plasmid isolation using PureLink HiPure Miniprep Kit (Thermo Fisher).
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bioRxiv - Cell Biology 2019Quote: ... Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific). 25 μL of 5 pM library DNA (1.25*10−4 pmol ...