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Citations for Thermo Fisher :
4501 - 4550 of 5129 citations for PLXDC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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bioRxiv - Microbiology 2024Quote: ... Eluting peptides were directly analyzed on a Q-Exactive HF mass spectrometer in data-dependent acquisition (DDA) mode (Thermo Fisher Scientific)15 ...
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bioRxiv - Cell Biology 2024Quote: The resulting peptides were analyzed by LC-MS using Nano LC-MS/MS (Dionex Ultimate 3000 RLSCnano System, Thermo Fisher Scientific) interfaced with Eclipse (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... sonicated and the peptide concentration was determined through absorbance at 280 nm measurement using a Nanodrop instrument (ThermoFisher, Nanodrop One C).
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bioRxiv - Microbiology 2024Quote: ... Isobaric-labelled peptides from total proteome and phosphoproteomes were then fractionated into eight fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Peptides were analysed through Ultimate 3000 RSLC nano-UPLC system connected with an Orbitrap Elite hybrid mass spectrometer (Thermo Fisher Scientific). Roughly 600 ng of peptides were loaded with automated injections onto a trap column (Acclaim PepMapTM 100 ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and incubated 1 hour at RT with secondary antibodies diluted in blocking solution as follows: anti-guinea pig Dylight 488 (Thermo Scientific, SA5-10094) (0.5 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Staining media was prepared in blocking buffer using alpha-Bungarotoxin (BTX) conjugated with Alexa Fluor 555 (ThermoFisher, B35451, 1:200 dilution in PBS). Additionally ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked with 600 μL blocking buffer (33% sonication buffer, 67% 2x hybridization buffer, 500 ng/μL yeast RNA [Thermo Fisher Scientific], 1% BSA) at room temp for 1 hr ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexaflour 594 (Life Technologies, 1:500) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexafluor 594 (Life Technologies, 1:500) for 1 hour at room temperature.
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bioRxiv - Physiology 2020Quote: ... Slides were subsequently exposed to a 1:5000 dilution of the primary antibody in blocking solution (Alexa488-conjugated rabbit anti-GFP; Life Technologies, Waltham, MA) for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody diluted in blocking solution for 1-2h (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies). Finally ...
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bioRxiv - Bioengineering 2020Quote: ... Slides were blocked in serum-based blocking buffer and incubated with primary antibody against MMP12 (MA5-32011, Invitrogen, 1:50, 20 μg/mL). Slides were washed in PBS (3×5 minutes) ...
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bioRxiv - Biophysics 2021Quote: ... After one hour of blocking with 5% (w/v) skim milk in 0.1% (v/v) Tween™-20 (Fisher Scientific, Hampton, NH, USA) and PBS (PBS-T) ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neuronsbioRxiv - Developmental Biology 2021Quote: ... cells were washed with 0.1% PBST and further incubated with blocking serum containing the corresponding secondary antibodies (Streptavidin Alexa Fluor™ 488, 546 or 647 conjugates; Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in 1 mL the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000, Hoechst 33342 at 1:1000 [Thermofisher] and Phalloidin-AlexaFluor555 at 1:200 [Thermofisher] in blocking buffer). The tissue was washed extensively and then mounted on glass slides using VectaShield (Vector Labs ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then permeabilized using 0.1%Triton X-100 for 10 minutes and blocked using Seablock blocking buffer (37527, Thermo Fisher Scientific, MA, USA) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Hemocytes were washed three times with blocking buffer and counterstained with 20 µM Hoechst 33342 (405, Molecular Probes, ThermoFisher Scientific, Waltham, MA, USA) and then mounted by adding 2 drops of Prolong Gold Antifade Mountant (Molecular Probes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with primary antibodies diluted with blocking buffer overnight at 4°C followed by washes and incubation with Alexa Fluor®-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the secondary antibody (1/400 in blocking buffer, goat anti-rabbit Alexa 594 antibody, Thermo Fisher, A-11037 or donkey anti-mouse Alexa 488 antibody, Thermo Fisher, R37114) was added in blocking buffer for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
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bioRxiv - Developmental Biology 2022Quote: ... The samples were washed (4 times 30 min, PBST) and then incubated with the secondary antibody (Alexa fluor 647, 1: 1200 ThermoFisher in blocking solution) overnight at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were incubated for 30 min in blocking buffer containing 2% (w/v) BSA and then incubated with Alexa Fluor 488 phalloidin (1:400 dilution, Thermo Fisher Scientific, A12379) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 0.02M KPBS and incubated for 2 hr at room temperature in blocking buffer containing goat anti-rabbit Alexa-Fluor conjugated secondary antibodies (Life Technologies, Carlsbad, CA). Sections were coverslipped using ProLong antifade mounting medium (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated for 2 hr at room temperature in blocking buffer containing secondary antibodies against rabbit (raised in goat) conjugated with Alexa-Fluor fluorochromes (Life Technologies, Carlsbad, CA) and coverslipped using ProLong mounting medium (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific, PA1-933) and mouse anti-GFP antibody (1:500 ...
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bioRxiv - Genetics 2019Quote: ... primary antibodies in blocking buffer containing 5% milk followed by the secondary antibodies of goat anti-mouse IgG (H+L) HRP (Life Technologies, 1:10000) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... washed with PBS and then blocked with blocking solution composed of 10% normal donkey serum or normal goat serum (both from Thermo Fisher Scientific Inc.) and 0.1% Triton-X-100 in PBS for one hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated for 30 minutes with the appropriate secondary antibodies (1:400 dilution in blocking buffer) and/or Alexa FluorTM-647 conjugated phalloidin (1:400, Invitrogen, A22287, lot 1884190), and washed twice more with PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Sections were washed 3x in 1x PBS and bound primary antibodies were detected by incubation with Alexa-fluor conjugated secondary antibodies diluted in blocking buffer (Thermo Fisher/Invitrogen, Eugene, OR) for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then washed with PBS and incubated with blocking buffer (10% goat serum in PBS) for 30 min at RT and incubated with primary antibody (anti-GFP Invitrogen chicken Y fraction) at 1:1000 in blocking buffer 4 h RT ...
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bioRxiv - Biochemistry 2022Quote: ... coverslips were washed 3 x 10 min in PBS containing 0.1% Triton X-100 at room temperature and then incubated for 1 hr at room temperature with secondary antibodies diluted in blocking buffer: AlexaFluor594 goat anti-mouse IgG (Molecular Probes, ThermoFisher Scientific, AB_2534091) and AlexaFluor488 Streptavidin (Molecular Probes ...
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bioRxiv - Bioengineering 2022Quote: ... Organoids sections were washed three times for ten minutes in 1X PBS prior to a two-hour incubation in 10% BSA in PBS blocking solution (ThermoFisher Scientific BP1605-100). The sections were then incubated in primary antibodies diluted in blocking solution overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and lung cryosections after blocking were stained with a 1:200 dilution of Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific, Rockford, IL, USA) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Virus infected cells were detected by staining with a primary rabbit anti-VACV antibody (1:1000 in blocking buffer for 1 hour, Thermo Fisher PA1-7258) and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed 5x with blocking solution and mounted with Invitrogen ProLong™ Gold antifade reagent with DAPI (Thermo Fisher, MA, USA). Slides were imaged with an Olympus FV3000 confocal microscope.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed 5x with blocking solution and a 1:500 dilution of secondary antibodies (Invitrogen Goat anti-rabbit IgG (H+L) Superclonal™/Invitrogen Alexa Fluor™ 594 goat anti-mouse IgG2a (γ2a ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were added overnight at 4°C in blocking buffer: Alexa Fluor™ 647 donkey anti-rabbit IgG (1:1000, Invitrogen, #A-32795), Alexa Fluor™ 488 donkey anti-mouse IgG (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were washed for 1 hour in 1x PBS twice at room temperature and then incubated in a solution containing 1% Triton X-100 (93443, MilliporeSigma) in StartingBlock (PBS) Blocking Buffer (37538, ThermoFisher Scientific, Waltham, MA) overnight at room temperature to permeabilize and block the tissue ...
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bioRxiv - Neuroscience 2022Quote: ... the fixed tissue cultures were incubated for 1 h at room temperature in blocking solution consisting of 10 % (v/v) normal goat serum (NGS; Fisher Scientific, Cat# NC9270494) and 0.5 % (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at RT and then in the blocking buffer containing monoclonal anti-GFP mouse antibody (GF28R, 1:3000, Thermo Fisher, #MA5-15256) and biotin-conjugated anti-ALFA nanobody (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with blocking solution and incubated with species-specific Alexa Fluor 488 and 555 conjugated secondary Abs (1:200) (Invitrogen, Carlsbad, CA, USA) and DR™ nuclear stain (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich], and 0.05% Tween 20 [Thermo Fisher Scientific]) was added and plates incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...