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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: siRNA for mouse-IGF1R was constructed in vitro using the Silencer siRNA Construction Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cochlear RNA was extracted from C57BL/6J mouse with Micro Scale RNA isolation Kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and other mediators by 22-plex ProcartaPlex mouse basic kit (Thermo Fisher Scientific, Waltham, USA) on a Luminex-200 instrument (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from the PhaZ-producing strains (RNA/DNA purification kit, Norgen Biotek for C. testosteroni, and GeneJET Genomic DNA Purification Kit, ThermoFisher Scientific for other strains). Inserts were obtained by amplifications of the mature phaZ genes (without signal peptides ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin C (primary: mAb 14D12, Abcam catalog no. ab106046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α- IgM (mAb II/41 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated at 4°C overnight with the primary antibodies (Mouse anti-Dyrk1a: Abnova, Cat. N. H00001859-M01; Rabbit anti-Camk2a: Molecular probes, PA5-14315). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... Ten milligrams (10 mg) of each mouse liver stored at −80°C was homogenized in TRIzol RNA isolation reagent (Invitogen-Thermo Fisher, Tokyo Japan) and pooled into 4 sample tubes (Con ...
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bioRxiv - Genetics 2023Quote: Mouse Embryonic Fibroblasts were obtained from E12.5 mouse embryos and expanded for 10 days at 37 °C with 5% CO2 and 5% O2 in DMEM medium (Thermo Fisher Scientific, 31966021) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Mouse α-syn monomers (Proteos, #RP-009) were dialysed against ultrapure water at 4°C overnight using microdialysis tubes (Thermo Fisher Scientific, #69580) with 2-3 buffer replacements to remove the stock buffer solution ...
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bioRxiv - Neuroscience 2023Quote: Samples were then washed and incubated in the relevant secondary antibodies for 24 hours at 4°C.: Alexa FluorTM 647 goat anti-mouse (Thermo-Fisher Scientific, A-21235) and Alexa FluorTM 488 goat anti-chicken (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Immunology 2021Quote: ... Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad ...
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bioRxiv - Neuroscience 2021Quote: ... rRNA were depleted from 1 μg of total RNA using the NEBNext rRNA depletion kit (Huma/Mouse) and ribo-depleted RNA was quantified by Quant-iT ™ RiboGreen ™ RNA Assay Kit (Invitrogen). Using a reverse transcriptase and random primers ...
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bioRxiv - Molecular Biology 2019Quote: ... quickly washed with PBS+ before incubating with 70 µl of secondary antibody mix (Goat anti-mouse Alexa Fluor® 488 or Goat anti-mouse Alexa Fluor® 594 1/400 in PBS+, Invitrogen) for 1 h at RT in a moist chamber ...
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bioRxiv - Physiology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine, Invitrogen, T2762) for 45 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies used were goat anti-mouse IgG2b 594 and goat anti-mouse IgG1 647 (all from Molecular Probes, 1:500). After several washes in PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
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bioRxiv - Molecular Biology 2021Quote: ... and stored at −80°C until DNA extraction with a magnetic-based method (MagMAX CORE extraction kit – ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... for 2 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific). DNA fragmentation range was assessed by electrophoresis.
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bioRxiv - Molecular Biology 2021Quote: ... for 4 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific). DNA fragmentation range was assessed by electrophoresis.
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bioRxiv - Plant Biology 2020Quote: ... and afterwards digested overnight at 37 °C with trypsin (Sigma; in-gel tryptic digestion kit, Thermo Fisher Scientific), desalted with C18 Ziptips (Millipore) ...
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bioRxiv - Genetics 2020Quote: mRNA was extracted from AX2 cells stored at −80 °C using Dynabeads mRNA direct micro purification kit (Invitrogen). Samples were lysed using tissue homogenizer (Kontes ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was subjected to bisulphite-meidated C to U conversion using MethylCode Bisulfite Convrsion Kit (ThermoFisher Scientific), and then used as a template for PCR for 35 cycles with EpiTaq (TAKARA ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed during 30 min at 37°C following the manufacturer’s protocol (TURBO DNase, kit Invitrogen), except that RNasine 0.36 U/μL (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... and Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C-10424) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was reverse transcribed for 30 min at 50°C using the SSIII One-Step Kit (Thermo Fisher) followed by 45 PCR cycles of 94°C for 15 seconds ...
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bioRxiv - Genetics 2022Quote: ... DNA from the affected siblings in family C was exome sequenced using Ampliseq whole exome kit (ThermoFisher Scientific) and run on a Proton Sequencer ...
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bioRxiv - Genetics 2022Quote: ... Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific) or the Pierce HA Tag IP/Co-IP Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and the concentration determined using a BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). After running an SDS PAGE (12%) ...
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bioRxiv - Microbiology 2024Quote: ... fixed at 50°C for 50 minuftes and stained with LIVE/DEAD BacLight Kit (Molecular Probes-Invitrogen, USA). Biofilms were visualized by confocal laser scanning microscopy (CLSM-Leica SP8 inverse ...
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bioRxiv - Microbiology 2024Quote: ... fixed at 50°C for 50 minuftes and stained with LIVE/DEAD BacLight Kit (Molecular Probes-Invitrogen, USA). Biofilms were visualized by confocal laser scanning microscopy (CLSM-Leica SP8 inverse ...
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bioRxiv - Bioengineering 2024Quote: ... In vitro transcription was performed overnight at 37 °C with the MEGAshortscriptTM T7 Transcription Kit (Thermo Fisher Scientific) adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Purified C-extein was covalently linked to keyhole limpet hemocyanin using the Imject EDC mcKLH spin kit (ThermoFisher) for antibody production due to its small size (11kD) ...
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bioRxiv - Systems Biology 2019Quote: Liver endothelial cells were incubated for 1 h at 37°C with 1 µM Fura-2 AM (Thermo Fisher Scientific, USA) and 0.1% pluronic acid in Standard Bath Solution (SBS) ...
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bioRxiv - Bioengineering 2019Quote: ... at 4°C followed by AlexaFluor-568 antibody (1:500) and concurrently with AlexaFluor-647-conjugated bungarotoxin (1:1000, Invitrogen, B35450) for two hours at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... NB100-2284) overnight at 4°C followed by AlexaFluor-568 antibody (1:500) or AlexaFluor-568 antibody (1:500, Invitrogen, A10087), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
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bioRxiv - Neuroscience 2020Quote: ... Third instar larvae were dissected with cold HL3 and immediately fixed with PFA (4%) and incubated overnight at 4 C with primary antibodies (rabbit anti-Dlg, 1:1000; anti-Brp 1:100, Life Technologies). Alexa-conjugated secondary antibodies were used for secondary staining (Jackson Laboratories 1:500) ...
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bioRxiv - Microbiology 2021Quote: ... antibody 1:3000 in 1% PBS/BSA at 4°C followed by secondary antibody conjugated to Alexa Fluor 555 (Thermo Fisher) at 1:500 in PBS/BSA 1% was performed ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM 2-mercaptoethanol and incubated for 1 h at 37 °C with 1 mM EDTA (ethylenediaminetetraacetic acid) and RNase A/T1 (ThermoFisher Scientific) (1:100) ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of culture was added to 1 mL of Trizol pre-warmed to 37°C (Life Technologies, Carlsbad, CA, USA) and incubated at room temperature for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041; AlexaFluor 647, 1:200, Invitrogen, A21236). Whole muscles were dehydrated in a series of 25% ...
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bioRxiv - Microbiology 2024Quote: ... Scicons) for 1 hour at 37 °C and then with Alexa Fluor 594-conjugated secondary antibodies (1:2000; Thermo Fisher Scientific) dissolved in DAPI solution for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C for 18 hours with primary antibodies: E-Cad (13-1700, Thermo Fisher, HECD-1, 1:100) and CK-14 (RB-9020 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...