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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Neuro2A-cells (ATCC #CCL-131) were cultured in six-well plates (106 per well) in DMEM + GlutaMAX-1 (25300-054/096, GIBCO by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Immunology 2024Quote: ... IgGs from individual serum or pooled sera bound to the plates were detected after a 2-h incubation with goat anti– mouse-IgG–linked horseradish peroxidase (1:8,000 Invitrogen, USA). For measuring IgG isotypes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were grown in µ-Slide 8 well high ibiTreat (80806 Thistle Scientific) or 96-well plates to approximately 60% confluency and preloaded with 1 µM Fluo4-AM (F14201, Invitrogen) /0.02% Pluronic® F127 in HBSS ...
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bioRxiv - Developmental Biology 2024Quote: ... Five droplets were added to a 12-well plate and covered with 1 mL of TOM [Advanced DMEM/F12 (Gibco), 1x N2 supplement (Gicbo) ...
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bioRxiv - Immunology 2024Quote: ... plates were rewashed and incubated with HRP-conjugated goat anti-hamster IgG (H+L) secondary antibody (HA6007; Invitrogen; 1:1500) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Tissue was added to 96-well round bottom plates in 100μL of R10 (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin, 1X Glutamax (Thermo Scientific), and 55μM b-mercaptoethanol) ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15 µL of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15ul of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Bioengineering 2024Quote: ... the plates were incubated with horseradish-peroxidase conjugated goat anti-mouse IgG (diluted 1/5,000) secondary antibodies (ThermoFisher 62-6520) for 1 h at room temperature ...
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bioRxiv - Biophysics 2024Quote: Fluorogenic POP substrate Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin (AMC) (HANGZHOU JHECHEM CO LTD) was diluted in PBS to 1-25 µM final concentrations in 96-well plates (ThermoFisher) to measure enzyme activity parameters in real time and as end-point reactions at 10 and 30 min ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Cell Biology 2023Quote: ... Both human and mouse hepatocytes were plated on collagen I coated tissue culture plate at a density of 1×105/cm2 in William’E medium (Thermo Fisher) and 10% FBS (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Genomics 2023Quote: ... EBs were plated on polyornithine/laminin-coated plates and cultured in N2/B-27 media containing 1 μg/mL laminin (Invitrogen). Neural rosettes were harvested by treating 14-day-old EBs with STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... stained bacteria were fed to ciliates using a 1:200 protist:bacteria ratio and transferred to a 96 well black plate (Thermo Scientific). DCF fluorescence was monitored automatically by measuring green fluorescence with 480 nm excitation and 512-552 nm emission wavelengths with a TECAN Infinite MPlex plate reader every 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... Compound solutions were then dispensed at 10nl/well directly into columns 1–22 of 384-well assay plates (Nunc 242757), while columns 23 and 24 were reserved for controls (see below) ...
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bioRxiv - Biochemistry 2022Quote: ... homogenized and counted to seed 7.5*105 cells in 1 mL of growth media per well in a 12-well plate (#55428, Nunc/DDBioLab) and incubated overnight at 23°C ...
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bioRxiv - Biochemistry 2022Quote: ... homogenized and counted to seed 7.5*105 cells in 1 mL of growth media per well in a 12-well plate (#55428, Nunc/DDBioLab) and incubated overnight at 23°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in double filtered sea water for 60 minutes on a shaking plate followed by three 1 minute 1X PBS Tween (1X Phosphate Buffered Saline: Gibco #70011-044 ...
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bioRxiv - Immunology 2023Quote: ... Between 1×106 and 1×107 splenocytes were transferred to 96-well plates for staining with freshly prepared LIVE/DEAD Aqua (Invitrogen) and TruStain FcX (anti-mouse ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: HepG2 cells and HepG2DNAJB1-PRKACA clones were transfected in 96-well plate with 0.25-100 nM Silencer small interfering RNA (siRNA) (Negative Control: #1, CDK7: s2830) (Life Technologies) using Lipofectamine RNAiMAX transfection reagent (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were thawed onto PLO and laminin-coated plates and cultured in astrocyte media supplemented with 1% knockout serum replacement (KSR)(Thermofisher). Astrocyte progenitor cells (APCs ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mM EDTA pH 8.0) and purity was assessed using the Multiskan SkyHigh Microplate Spectrophotometer and µdrop plate (Thermo Scientific). Sample purity was assessed by measuring the 260/280 nm and 260/230 nm absorption and only samples achieving 1.7-2.0 and 2.0-2.2 respectively were further evaluated.
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-1 HexaPro S) at 3 µg/mL were placed into 384-well Nunc Maxisorp plates (ThermoFisher, 464718) in 1x TBS and incubated for 1 hour at 37°C followed by slap drying and blocking with 80 µL of Casein for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: 293T IFNB12X-GLuc cells plated in 96-well plates were transfected with 1 pmol/well siRNA (Silencer Select, Thermo Fisher) using Lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were then diluted 1:1.000 in fresh medium and 200 μl were transferred into 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 15 min for 16 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was extracted from the femur and tibia of dissected mice and plated on non-tissue culture-treated 15-cm Petri dishes at a density of 1 x 107 cells per plate in macrophage differentiation medium (DMEM, Gibco) supplemented with 2mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
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bioRxiv - Neuroscience 2024Quote: ... in 6-well tissue culture plates containing 1 ml prewarmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
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bioRxiv - Immunology 2024Quote: Bd isolate JEL 197 was grown in 1% tryptone broth or on 1% tryptone agar plates (Difco Laboratories, Detroit, MI) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco) at 19°C.
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bioRxiv - Physiology 2024Quote: ... Monocytes were cultured in 12-well plates at a density of 1 × 106/mL in Roswell Park Memorial Institute media 1640 (Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated TACC3 Δ699-765 (50 µL of 10 µg mL−1, diluted in PBS) was immobilized on preblocked HBC Streptavidin plates (ThermoFisher) for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... into a single-cell suspension and seeded on Matrigel-coated plates with ROCK inhibitor and knockout serum replacement (1%, Gibco).
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bioRxiv - Microbiology 2024Quote: ... cells were seeded in a 24-well plate at a density of 105 cells per well in 1 ml MEM (Gibco) and grown for 5-6 days ...
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bioRxiv - Microbiology 2024Quote: ... Vero cells (1 × 105 cells per well) in a 24-well cluster plate were transfected with 1 μg plasmid DNA using the Lipofectamine 3000 system (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After another rinse and three more washes the plate was developed with 1-Step™ Ultra TMB-ELISA Solution (ThermoFisher) before stopping the reaction with an equal volume of 2M H2SO4 ...
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bioRxiv - Plant Biology 2020Quote: ... The amplicons were checked by electrophoresis and 25 µl of each pooled PCR product were purified and normalized using SequalPrep Normalization Plate Kit (Invitrogen GmbH, Karlsruhe, Germany). We then pooled the samples and the mock community and proceeded for a single library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing reactions were carried out on 96-well plates using BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems, foster City, CA) for 30 cycles ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from 10-day old seedlings grown on ½ MS plates under long-day conditions using PureLink RNA Mini Kit (Thermo Fisher Scientific, 12183025). One microgram of total RNA was reverse transcribed into cDNA with Protoscript II (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat. No. A1051001, ThermoFisher, Waltham, MA, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was produced from mRNA from cells lysed directly in the culture plate using the TaqMan® Gene Expression Cells-to-CT™ Kit (ThermoFisher Scientific). qRT-PCR was then carried out using the corresponding TaqMan™ Gene Expression Assay Kit that include exonspanning probes for genes of interest (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 37°C for 24 hr and stained with a Filmtracer LIVE/DEAD Biofilm Viability Kit (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: 10 μL of PCR product for each sample was normalized using a SequalPrep 96-well Normalization Plate Kit (Thermo Fisher Scientific, Waltham, MA). Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: DNA in 100 µl of undiluted cell-free BALF (first wash) was quantified in DNase-free 96-well plates (TPP®) using Quant-iTTM Pico GreenTM dsDNA Assay Kit (Thermo Fisher Scientific) following manufacturers’ instructions.
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bioRxiv - Zoology 2019Quote: Ten microliters of PCR amplicon was purified and normalized for concentration prior to library preparation using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, USA). DNA libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs ...