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Citations for Thermo Fisher :
4451 - 4500 of 6574 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Immunology 2023Quote: ... A goat anti-Human IgG secondary antibody labelled with Alexa Fluor 488 was added (Thermo Fisher Scientific). The plates were incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-labeled goat anti-human IgG secondary antibody (Invitrogen, A18817) at 37 °C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Anti-mouse ZAKa antibody (proteintech, #14945-1-AP) and anti-human ZAKa antibody were from Thermo Fisher Scientific (A301-993A) ...
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bioRxiv - Immunology 2023Quote: ... Cytokine release in the supernatants was measured with an Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen) and was analyzed with the DropArray (Curiox Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and S-D614G-N149Q were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using a HisTrap FF column (GE Life Sciences)(64 ...
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bioRxiv - Microbiology 2023Quote: ... Secreted antibodies were detected with 1µg/mL goat anti-human IgG (H+L)-Alexa Fluor 594 (Invitrogen), which was added to the cell culture media used to resuspend the antigen-coated beads.
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bioRxiv - Microbiology 2023Quote: ... and 50 ng of human ACE2-encoding plasmid or an empty plasmid control using Lipofectamine 2000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... human primary T cells and DG-75 cells were electroporated using the Neon Transfection kit (ThermoFisher Scientific) at 1600 volts / 10 ms / 3 pulses ...
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bioRxiv - Cell Biology 2023Quote: ... Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) (Life Technologies Green Island NY) media ...
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bioRxiv - Cell Biology 2023Quote: ... Human primary keratinocytes were transfected at 20 nM final siRNA concentration using Lipofectamin 2000 reagent (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... and TOMM22) were amplified using an Ion AmpliSeq Transcriptome Human Gene Expression Core Panel (Thermo Fisher Scientific). Amplicons were ligated to barcode adapters and purified using Agencourt AMPure XP reagent (Beckman Coulter Inc.) ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were probed with rabbit monoclonal anti-human CD38 antibody (Clone RM388, Thermo Scientific, cat# MA5-36061) after blocking with 5% BSA ...
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bioRxiv - Cell Biology 2023Quote: Immortalized human retinal pigment epithelial cells (RPE-1hTERT, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK) 293T and Vero cells were grown in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: A synthetic sequence of human Twisted gastrulation (residues Cys26-Phe223) codon optimised for mammalian expression (ThermoFisher Scientific) with a C-terminal TwinStrep-tag (IBA LifeScience ...
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bioRxiv - Biophysics 2024Quote: Human mesenchymal stem cells (hMSCs) (ATCC, lgc-standards, Teddington, UK) were cultured in high-glucose DMEM (Gibco) supplemented with 10% FBS and 1 % penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... or ON-TARGETplus Human SUSD3 SMARTPool siRNA (Dharmacon #L-016811-02-0005) using Lipofectamine RNAiMAX (Invitrogen #13778) protocol for 24 or 72 hours.
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bioRxiv - Cell Biology 2024Quote: Human A549 lung carcinoma cells (ATCC, CCL-185) were maintained in Gibco DMEM/F-12 (Thermofisher 11320033) with 100 U/ml penicillin ...
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bioRxiv - Biochemistry 2024Quote: The human derived NCH612 glioma cell media was prepared using 500 mL DMEM: F12/Glutamax media (Gibco) with the following additives ...
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bioRxiv - Biochemistry 2024Quote: Synchronized or treated human embryonic kidney (HEK293T) cells were lysed in RIPA buffer (Thermo Fisher Scientific # 89901) containing 10mM of sodium butyrate ...
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bioRxiv - Biophysics 2024Quote: Human-derived neuroblastoma cells (SH-SY5Y, ATCC Product Number: CRL-2266) were cultured in DMEM-F12 (Invitrogen) medium supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD8+ T cells were left unstimulated or were stimulated with anti-CD3/CD28 dynabeads (ThermoFisher) at a 1:1 bead-to-cell ratio for 6 ...
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bioRxiv - Microbiology 2024Quote: Human jejunal enteroids and colonoids were cultured in Matrigel (75%) domes overlaid with hOGM/1x PenStrep (Gibco) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... and Taqman gene expression assays (human tyrosinase [TYR] Hs00165976_m1; mouse TH Mm00447557_m1; Slc6A3 [DAT] Mm00438388_m1; Slc18a2 [VMAT2] Mm00553058_m1, Applied Biosystems) using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated using the DynabeadsTM untouched human CD8 T-cell isolation kit (Invitrogen), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™ ...
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bioRxiv - Developmental Biology 2024Quote: ... and adapter ligation were performed using an Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The cDNA library was purified using Agencourt AMPure XP Reagent (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... Human primary Müller cells (P3) at 80% confluency were transfected with MT1 (assay ID: s194623, ThermoFisher Scientific), AKAP12 (assay ID ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in DMEM supplemented with 10% fetal calf serum (Gibco). Cells were seeded with 300 000 cells/well in 6-well plates and transfected 24 hours later with 1 µg of each minigene construct mixed with 3 µL of Lipofectamine 2000 Reagent (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Human skin: Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco) digestion for 20h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... falciparum blood-stage parasites were cultured in 2.5% hematocrit human RBC in malaria culture media (MCM) comprising of 10.43g RPMI 1640 powder (Gibco), 25ml 1M HEPES (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... The silencer select siRNA targeting human ERK3 and the Silencer non-targeting Control #1 were purchased from Ambion.
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2020Quote: Normal human fibroblast cell line (AG08469, Coriell Institute for Medical Research) was maintained in Minimum Essential Medium (Gibco) and supplemented with 15% FBS (HyClone) ...
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bioRxiv - Cell Biology 2020Quote: Silencer Select siRNAs against human SNX4 and VPS35, and nontargeting control “scrambled” siRNA (predesigned, 4,390,844) were purchased from Ambion® (Thermo Fisher Scientific) ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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bioRxiv - Genomics 2021Quote: Human BLaER1 cells (14) were kindly provided by Thomas Graf (CRG, Barcelona) and grown in RPMI medium (Invitrogen) supplemented with 10% heat-inactivated foetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was maintained in feeder-free culture on Geltrex matrix (A1413202 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human CD171 (i.e. antibody against neural cell adhesion molecule L1CAM; RRID: AB_2043813; Thermo Fisher Scientific, Waltham, MA) or anti-human tetraspanin CD81 (Ancell Corporation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Microbiology 2022Quote: ... falciparum was cultured in O+ human RBCs (Interstate Blood Bank, TN) supplemented with RPMI1640 medium along with 0.3-0.5% Albumax I (Invitrogen), 2g/L sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: ... Human HAP1 cells were obtained from Horizon Discovery and were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco), containing L-glutamine and 25 nM HEPES and supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... 19-805) of human ACE2 (GenBank NM_021804.1) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA) ...
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bioRxiv - Molecular Biology 2020Quote: ... we used TaqMan probes for human HTT and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs obtained from Applied Biosystems. Relative HTT gene expression was determined using the ddCt method ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: Human ISYNA1 isoform 2 (UniProt classification) was cloned into a pcDNA4/TO mammalian expression vector (Thermo Fisher Scientific). The cDNA was amplified by PCR (forward primer ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...