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Citations for Thermo Fisher :
4451 - 4500 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Sybr green assays were also used for human or mouse gene expression with SYBR Green Master Mix (2x, Thermo Fisher Scientific). Primer sequences are as follows:
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bioRxiv - Immunology 2021Quote: ... Cells were washed once and resuspended in phenol-free RPMI with 2% (v/v) human AB serum containing DAPI (ThermoFisher 62248). Single-color controls were performed with these same antibodies using BD CompBeads (BD Biosciences 552843) ...
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bioRxiv - Neuroscience 2021Quote: ... From live single cells with top 30% protein expression (evaluated by EGFP signal) the MFI of the Alexa Fluor 647-conjugated goat anti-human IgG (1:400, Life Technologies) was calculated.
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bioRxiv - Cell Biology 2021Quote: The expression cassette consisting of a Kozak sequence followed by the coding sequence for human diphtheria toxin receptor (hDTR; UniProt Q53H93) was codon-usage optimized for expression in mice and synthesized (Thermo Fisher Scientific/GeneArt ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Microbiology 2020Quote: ... human epithelial cell line were cultured at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, Waltham, MA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted and the whole transcriptome of ALDH+ and ALDH-populations was assessed using Affymetrix Whole-Transcript Human Gene 1.0 ST Array (Affymetrix, Thermo Fisher Scientific) (30) ...
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bioRxiv - Neuroscience 2022Quote: Healthy wild type control (WT) iPSCs derived were obtained from Thermo-Fisher Scientific (WT, GIBCO human episomal iPSC line, cat # A18945), which were generated via reprogramming of cord blood derived CD34+ progenitors ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs were extracted from the human prostate tumors and the normal tissues and various cell lines using Trizol (Life Technologies). Reverse transcription was performed using the M-MLV reverse system (Takara ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were maintained in monolayer cultures of human foreskin fibroblasts (HFFs, ATCC® SCRC-1041) in Dulbecco’s Modified Eagles Medium (DMEM, GIBCO, USA) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5.5μg of fragmented cDNA was hybridized on Affymetrix GeneChip™ Human Transcriptome Array 2.0 (HTA2.0) chips (Invitrogen, 902162, lot no. 4364589) for 16 hrs at 45°C ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Cell Biology 2022Quote: ... Transcriptional profiling was performed with the use of the Affymetrix Human Genome U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA). All labeling reactions and hybridizations were carried out according to the standard GeneChip® eukaryotic target labeling protocol ...
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bioRxiv - Cell Biology 2022Quote: ... DNAs were amplified by PCR from human Caco2 cells or MCF7 breast cancer cells and cloned into Gateway™ expression vectors (Invitrogen) according to the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat polyclonal antibodies conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/100 in FACS buffer ...
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bioRxiv - Genomics 2022Quote: PBMC culture supernatants were analyzed for secreted proteins with Immune Monitoring 65- Plex Human ProcartaPlex Panel (Thermo Fisher Scientific, Carlsbad, CA) as per manufacturer’s instructions (all proteins assayed shown in Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2.5 µg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes [65] using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 pmol of desthiobiotinylated HOXC13-AS was mixed with magnetic beads and incubated with 200 µg of protein lysate from human adult epidermal keratinocytes (ThermoFisher Scientific). Poly(A)25 RNA ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2/UT-NCGM02/Human/2020 (Wuhan strain) was propagated in VeroE6/TMPRSS2 cells in VP-SFM (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA levels were normalized to mouse Gapdh or human RPL22 cDNA and relative transcription levels were analyzed using the comparative ΔΔCt method (7500 Software V2.0.5, Applied Biosystems, Life Technologies). The Lgmn mRNA level of the microglia-enriched fraction was obtained from a NanoString dataset (Omnibus GSE129709 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129709)10.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed 3x in FACS Buffer and stained on ice for 1 hour with goat anti-Human IgG Fc Alexa Fluor™ 647 secondary antibody (ThermoFisher), protected from light ...
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bioRxiv - Immunology 2024Quote: ... primed NK cells from PWH were cultured with autologous CD4+ T cells also isolated by negative immunomagnetic selection (DynaBeads Human CD4+ T cell isolation kit, Life technologies) in the presence of 50nM Romidepsin (Selleck Chemicals ...
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bioRxiv - Pathology 2024Quote: ... and slides were analyzed independently by 3 experienced reviewers to identify and measure the area of human cardiomyocyte grafts using an EVOS (Life Technologies) microscope ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... samples were washed in PBS and a mixture of goat anti-Human Alexa488 conjugated secondary antibody (Thermo Fisher, diluted 1:2000) and DAPI (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Madin Darby canine kidney (MDCK) cells and human embryonic kidney 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Immunology 2024Quote: ... T cells (1 million cells/ml) were activated with Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) in culture medium ...
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bioRxiv - Microbiology 2024Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Approximately 800,000 U2OS cells were reverse transfected with 20 nM siRNA against the 3’ UTR of endogenous TIRR or a negative control siRNA (Dharmacon ON-TARGETplus Human NUDT16L1 siRNA J-014872-23-0005 or ON-TARGETplus Non-targeting Control) using 3 uL of RNAiMAX lipofectamine (Thermo Fisher) in a 6-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... Human peripheral blood mononuclear cells (ATCC #PCS-800-011) were thawed as recommended and resuspended in RPMI 1640 medium (Gibco #11875093) with 1X GlutaMAX (Gibco #35050061) ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... #D-001810-10-05) or human GPX3 siRNA (Dharmacon, #L-006485-00-0005) were delivered to the cells using Lipofectamine RNAiMAX (Invitrogen, 13778150). After 24 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20 ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Neuroscience 2023Quote: eGFP/Luc human iPSC-NSCs were seeded at a density of 1 x 104 cells per well in a Geltrex (Life Technologies) coated 96-well plate (flat bottom ...
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bioRxiv - Neuroscience 2023Quote: Low-passage-number Human embryonic kidney cells transformed with SV40 T-antigen (HEK293T) cells were cultured in DMEM (Thermo Fisher Scientific) with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Biophysics 2023Quote: ... University of Belgrade-Faculty of Biology which was previously produced by several reactions of cloning and subcloning of CTG repeats originating from human DMPK locus using pJet1.2/blunt cloning vector from CloneJET PCR Cloning Kit (Thermo Fisher Scientific). The subcloned region sequence was verified with Sanger sequencing before prior use ...