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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Mouse Cd28 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were expanded with anti-CD3/anti-CD28 (Thermo Fisher) beads at a 1:1 ratio for 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Stimulation with αCD3/CD28 DynaBeads Human T cell activator (Invitrogen) (cell to bead ratio of 1:1 ...
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bioRxiv - Immunology 2024Quote: ... They were then activated overnight by CD3/CD28 Dynabeads (Invitrogen) in complete X-Vivo media supplemented with 100 IU/ml IL2 (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with human CD3/CD28 Dynabeads (Life Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Penicillin-streptomycin and Hepes +/− anti-CD3/CD28 stimulation (Invitrogen Dynabeads) at a cell:bead ratio of 4:1 ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-Tregs were then activated with CD3/CD28 Dynabeads (Thermofisher) at a ratio of 1:1 in media composed of X-VIVO15 (Lonza ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2022Quote: ... from normal donors were obtained with an NIH-approved protocol and activated with CD3 and CD28 microbeads at a ratio of 1:3 (Dynabeads Human T-Expander CD3/CD28, Thermo Fisher Scientific, catalog no. 11141D) in AIM-V media containing 40 IU/mL recombinant IL-2 and 5% FBS for 24 hr ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...
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bioRxiv - Molecular Biology 2020Quote: ... The Mex67-3Flag tagged protein was isolated using 200 µL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 34 µg of anti-Flag M2 mouse monoclonal antibody (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... for 1 hour at room temperature followed by additional 1 hour incubation with 1:200 dilution of fluorescence-tagged goat anti-mouse IgG Alexa Fluor 488 (Thermofisher) (Supplemental Table 5) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS and incubated in Alexa Fluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were either cultured alone or with T cell activation beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Thermo Fisher Scientific, 11456D). After 72 hrs of culture ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were either cultured alone or with T cell activation beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Thermo Fisher Scientific, 11456D). After 72 hrs of culture ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat. #MA1-21315-1MG, ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Protein detection was performed by incubation with a 6x-His Tag mouse monoclonal antibody (clone: HIS.H8; Thermo Fisher Scientific), followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... 0.3μg of GFP-tagged construct DNA were mixed with 0.7μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1μg of GFP-tagged construct DNA were mixed with 0.2μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of 6xHis-tagged proteins and Strep-tagged proteins was tested with 6xHIS TagAntibody HIS.H8 (Invitrogen Co.) and StrepMAB-Classic ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg of GFP-tagged construct DNA were mixed with 0.5µg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then incubated with fluorescently tagged secondary antibodies and/or fluorescently tagged Phalloidin (ThermoFisher Scientific, Waltham, MA) diluted in the blocking buffer at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescently tagged secondary antibodies were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Alexa 488-tagged phalloidin (ThermoFisher, 400x stock) was added at 4x working concentration and incubated for 2 days ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged Fzd4 in pcDNA3.1 (Thermo Fisher), or both Tspan12 and Fzd4 (300 ng each receptor per mL of cells) ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor-tagged secondary antibodies (Invitrogen) were matched to the primaries and incubated for 2 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... T cells were activated using anti-CD3ε/CD28 Dynabeads (Thermo Fisher) at a bead-to-cell ratio of 1:1 then seeded onto the MSC at a density of 1.0×105/well (ratio 10:1 ...
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bioRxiv - Immunology 2021Quote: ... and 10 µg/ml CD28 monoclonal antibody (clone: CD28.2, Thermo Fisher) in the presence of 10 ng/ml TGF-β1 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2019Quote: ... and soluble anti-CD28 (1 µg/mL; eBioscience, Thermo Fisher Scientific) antibodies in the presence of 50 U/ml IL-2 (PeproTech ...
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bioRxiv - Immunology 2019Quote: ... or 3×105 CD3/CD28 Dynabeads/well (ThermoFisher Scientific, Waltham, MA). Cells were incubated for up to 4 days at 37°C ...
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bioRxiv - Genomics 2019Quote: ... 500 μL cRPMI with human T-Activator CD3/CD28 Dynabeads (Gibco) were added to each well at a ratio of 2 cells ...
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bioRxiv - Bioengineering 2020Quote: ... T-cells were stimulated with CD3/CD28 Dynabeads (Thermo Fisher Scientific) at 1:1 cell:bead ratio for 1 day before viral transduction ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were activated with anti-CD3/CD28 Dynabeads (Invitrogen) at a ratio of one bead per cell for 72 h and maintained in RPMI with 100 U/mL IL-2 (AIDS Reagents Program ...