Labshake search
Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Human EGFR protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
-
bioRxiv - Cell Biology 2022Quote: His8-tagged NR2B proteins were labelled with Alexa 647 NHS ester (Thermo Fisher) and PrLD-SAMWT/PrLD-SAMME/PrLD proteins labelled with Alexa 555 maleimide (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
-
bioRxiv - Plant Biology 2023Quote: ... The GST-tagged proteins were resuspended in phosphate-buffered saline (PBS) (Thermo Fisher) containing 1 mM dithiothreitol (DTT ...
-
bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected using SuperSignal West Femto: SuperSignal substrates (ThermoFisher Scientific) in a 1:1 ratio ...
-
bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific), RNAPII with antibody against Rpb1 (8WG16 ...
-
bioRxiv - Biochemistry 2024Quote: ... AncΔ5 were tagged with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher), followed by the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2023Quote: TRPM8 staining: PRP diluted in PBS was incubated with CD41a (anti-human, APC, Invitrogen) and TRPM8 antibody (1:250 dilution ...
-
Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
-
bioRxiv - Cell Biology 2019Quote: Proteins were fluorescently labeled for microscopy using Microscale Protein Labeling kits from Thermo Scientific: Alexa Fluor™ 488 (A30006) ...
-
bioRxiv - Biochemistry 2024Quote: ... AHA-labeled proteins were enriched with the Click-iT Protein Enrichment Kit (Invitrogen #C10416). For this ...
-
bioRxiv - Biochemistry 2021Quote: ... ACE2-Fc was purified over Protein A Agarose (Thermo Fisher; 15918014). Once the Protein A Agarose was equilibrated in PBS buffer ...
-
bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
-
bioRxiv - Genomics 2019Quote: ... The labeled DNA was then hybridized to Human Gene ST1.0 microarrays (Affymetrix), washed ...
-
bioRxiv - Microbiology 2020Quote: ... Labeled cRNA was hybridized to GeneChip Human Gene 2.0 ST Array (Affymetrix) for 16h at 45°C and scanned using the Affymetrix 3000 7G Scanner..CEL file generation and basic quality controls were performed with the GeneChip™ Command Console® (Affymetrix).
-
bioRxiv - Molecular Biology 2020Quote: ... PE-labeled mouse anti human CD63 antibody (12-0639-42, Life technologies), Alexa 647 labeled mouse anti human ICAM-1 antibody (sc-107 AF 647 ...
-
bioRxiv - Cell Biology 2019Quote: Alexa labeled Human Holo-Transferrin (Life Technologies, Invitrogen T23365, T2336; 5µg/ml) and Dextran (Life Technologies ...
-
bioRxiv - Cell Biology 2019Quote: Alexa labeled Human Holo-Transferrin (Life Technologies, Invitrogen T23365, T2336; 5µg/ml) and Dextran (Life Technologies ...
-
bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
-
bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2022Quote: ... we delivered recombinant Streptococcus pyogenes Cas9 protein (TrueCut™ Cas9 Protein v2; Thermo Fisher Scientific) together with the crRNA and tracrRNA into in vitro fertilized eggs of C57BL/6J mice according to the institutional procedure of Kyoto university (Ishibashi et al. ...
-
bioRxiv - Biophysics 2023Quote: ... and all subsequent EGFR constructs were made using the HA-EGFR plasmid and Platinum SuperFi II PCR Master Mix (Invitrogen, #12368010). Halo-EGFR constructs used for FRET-FLIM experiments were created using the HA-EGFR plasmid and the Gibson assembly approach to replace the N-terminal HA tag with the HaloTag® plasmid (Promega ...
-
bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
-
bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
-
bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
-
bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
-
bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded on human recombinant vitronectin in StemFlexTM medium (Thermo Fisher Scientific). iBEC differentiation was performed as previously described by us [18] ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
-
bioRxiv - Microbiology 2022Quote: ... human recombinant epidermal growth factor (0.2 ng/mL, all from Gibco, Schwerte, Germany), isoproterenol (1 nM ...
-
bioRxiv - Bioengineering 2022Quote: ... or 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700) for serum-free cells ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 µg/mL human recombinant insulin (ThermoFisher Scientific #12585014; stock = 4 mg/mL), 1:100 Penicillin-Streptomycin (ThermoFisher Scientific #15140122) ...
-
bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
-
bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
-
bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
-
bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
-
bioRxiv - Neuroscience 2021Quote: ... we labeled with anti-HuC/D (Elav protein) (Invitrogen A21271). Control (0.08% DMSO ...
-
bioRxiv - Biochemistry 2024Quote: Protein cages were labeled with AF647 Antibody Labeling Kits (Invitrogen). Over 500 µg of protein cage was incubated per reaction for 1 hour at 25°C with periodic inversion ...
-
bioRxiv - Immunology 2019Quote: ... conjugated to Alexa 488 and APC-labeled OVA conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Waltham, MA) and sorted for IgGpos/OVApos cells in a FACSAriaIII sorter (BD ...
-
bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
-
bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
-
bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
-
bioRxiv - Genetics 2022Quote: 3T3-L1 cells expressing GFP-tagged human CIDEC were incubated with MitoTracker (#M7512; Thermo Fisher Scientific) before fixation ...
-
bioRxiv - Immunology 2020Quote: ... and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells ...
-
bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...