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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Mouse IRX6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... were incorporated into pFastBac plasmid (Invitrogen) with octa-histidine tag combined with a Factor Xa cut site at the N-terminus and Flag-tag at the C-terminus ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were prepared in optimem (Gibco) and transfected using 6 ug of PEI max ...
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bioRxiv - Physiology 2024Quote: ... plasmids using Lipofectamine 3000 (ThermoFisher Scientific), following manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were blocked for one hour with mouse-on-mouse blocking reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Reagent, 1:30 dilution, ThermoFisher, R37621) between antibody staining procedures.
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid DNA was purified from miniprep cultures using a GeneJET plasmid DNA miniprep kit (Thermo Fisher, K0502). Constructs were checked with a control digestion performed using the same restriction enzymes used for ligation (BamHI and EcoRI) ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid DNA was extracted from these cultures using a GeneJET plasmid miniprep kit (Thermo Scientific, Dublin, Ireland) and sent to GATC (Konstanz ...
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bioRxiv - Cell Biology 2021Quote: ... The donor plasmid and the pX260 plasmid were co-transfected into ES cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The plasmid was isolated from transformed clones using the GeneJET Plasmid Isolation Kit (Thermo Scientific Inc, USA). Plasmid sequencing was outsourced to Bioserve Technologies Pvt ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection of horse PepT1 plasmid or empty plasmid was performed with 293fectin transfection reagent (Invitrogen/Thermo Fisher) as per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection of horse PepT1 plasmid or empty plasmid was performed with 293fectin transfection reagent (Invitrogen/Thermo Fisher) as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... or ABEmax)-encoding plasmid (0.7 μg) and each sgRNA expression plasmid(0.3 μg) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The injected DNA plasmids were prepared using PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Invitrogen, # A31231), aliquoted based on concentrations ...
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bioRxiv - Microbiology 2021Quote: ... This plasmid is derived from plasmid pMV001 that has been synthesized with GeneArt Gene Synthesis (ThermoFisher Scientific). pMV001 carries the Pada-ada-alkB operon where codons encoding Ada C38 and C321 have been replaced to encode A38 and A321 ...
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bioRxiv - Plant Biology 2019Quote: ... amplified from wild-type Col-0 cDNA downstream of the GFP coding sequence in the pB7WGF2 plasmid (Ghent plasmids collection, http://bccm.belspo.be/index.php) via Gateway technology (Invitrogen). The same was done for the p35S::GFP-S2Lb construct ...
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bioRxiv - Biochemistry 2019Quote: ... Colonies were scraped from the plate and plasmid DNA isolated with GeneJET Plasmid Miniprep Kit (Thermo Scientific). The isolated plasmid DNA was used to transform E ...
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bioRxiv - Systems Biology 2021Quote: ... 10 ng of each entry plasmid and destination plasmid was mixed with Gateway LR clonase II (Invitrogen) in a 4 μL volume and incubated at 25 °C for at least 16 hours ...
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bioRxiv - Systems Biology 2021Quote: ... 10 ng each of entry plasmid and destination plasmid was mixed with Gateway LR clonase II (Invitrogen) in a 2 μL final volume and incubated at 25 °C for at least 16 hours ...
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bioRxiv - Genetics 2021Quote: Plasmids were purified using the PureLink Fast Low-endotoxin Maxi Plasmid Purification kit (ThermoFisher Scientific, Cat. #A35895). All plasmids were sequenced prior to injection ...
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bioRxiv - Genetics 2020Quote: Plasmids were purified using the PureLink Fast Low-endotoxin Maxi Plasmid Purification kit (ThermoFisher Scientific, Cat. #A35895). All constructs were fully re-sequenced prior to injection ...
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bioRxiv - Immunology 2021Quote: ... Sequence verified plasmid encoding for mCD22-Fc was transfected together with Flp recombinase-expressing plasmid pOG44 (Invitrogen) using lipofectamine LTX (Invitrogen ...
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bioRxiv - Immunology 2022Quote: All the plasmids for analytical expression were purified using PureLink™ HiPure Plasmid Miniprep Kit (#K210003, Invitrogen). Plasmid vectors were transiently transfected into HEK293 FreeStyle cells using polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids were isolated from an overnight culture of single colonies using GeneJET Plasmid Miniprep Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid purification was carried out using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... and a control plasmid (pEGFP-C1 plasmid) using the Lipofectamine™ 3000 Transfection Reagent (ThermoFisher Scientific, USA) as instructed in the protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... we co-transfected 600 ng reporter plasmid and 200 ng PhiC31 integrase plasmid using Lipofectamine 2000 (Invitrogen). After selection by geneticin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were isolated from controls with a Purelink HQ Mini Plasmid Purification Kit (Invitrogen, Cat #K2100-01). Bacteria that were to be desiccated were concentrated by centrifugation (5,000 rpm ...
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bioRxiv - Systems Biology 2023Quote: ... Positive candidates were selected for plasmid DNA isolation using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The construction pDRF1-GW ymTq2Δ11 was confirmed by DNA sequencing (https://www.macrogen-europe.com/) ...
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bioRxiv - Microbiology 2023Quote: ... was used for routine cloning and plasmids were isolated using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). All the kits were used following the manufacturers’ protocol.
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bioRxiv - Microbiology 2023Quote: ... and 50 ng of human ACE2-encoding plasmid or an empty plasmid control using Lipofectamine 2000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA was isolated from the bacteria using PureLink HiPure Plasmid Filter Miniprep Kit (Thermo Fisher Scientific) and pooled to produce the final plasmid library.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-V5 mouse (Invitrogen, # R960-25, 1/1000), mouse anti-FLAG (Sigma ...
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bioRxiv - Immunology 2023Quote: ... PE-Cy5-conjugated mouse anti-mouse CD45.1 (A20; Thermofisher), PE-Cy5 or PE-CF594-conjugated rat anti-mouse CD45R/B220 (RA3-6B2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The plasmids were co-transfected with packaging plasmids (pspAX2 and pMD2G) into 293T cells using Turbofect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were sequenced and transfected along with lentiviral packaging plasmids into HEK293FT cells with Lipofectamine 3000 (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The cloned plasmids were then isolated from their bacterial cultures using GeneJET Plasmid Miniprep Kit from Thermo Fisher scientific (catalog number-K0503).
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bioRxiv - Immunology 2021Quote: ... Plasmid of positive clones were extracted using the GeneJET Plasmid Miniprep Kit following the manufacture protocol (ThermoFisher scientific). and tThe nucleocapsid cloning was confirmed by digestion with using BamHI and NheI FastDigest enzymes (ThermoFisher Scientific) ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Plasmids were extracted with Favorprep Plasmid Extraction Mini Kit and cleaved with FastDigest SacI restriction enzyme (Thermo Scientific) to eliminate the toxin plasmids ...
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... and total DNA amounts of 5μg (2.5μg donor vector plus 1.25μg of each TALENS arm plasmid) or 2μg (1μg piggybac vector plus 1μg of transposase plasmid) were combined with Lipofectamine Stem (ThermoFisher, STEM00003) in Opti-MEM (ThermoFisher 11058021) ...
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bioRxiv - Neuroscience 2020Quote: ... Large quantities of purified plasmids were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific). The cloned sequences of ItypOrco ...
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bioRxiv - Immunology 2019Quote: ... plasmids were treated with DpnI to remove non-mutated parental plasmids and cloned into Stbl2 cells (Life Technologies) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 5 × 104 E14Tg2a cells were transfected with 100 ng of reporter plasmids (pGL4.10[luc2] and their derivatives) and 1 ng of pGL4.70[hRluc] control plasmid using Lipofectamine 2000 (Invitrogen) in 96-well plates ...
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bioRxiv - Biophysics 2019Quote: ... were selected for their suitability for transformation with a plasmid containing ampicillin resistance (pRSET/EmGFP plasmid; Invitrogen, UK).
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bioRxiv - Neuroscience 2019Quote: ... human Ad5 genomic plasmid (pBHGfrtΔE1,3FLP; Microbix) and each pDC511-LRRK2 shuttle plasmid were introduced by calcium phosphate (Invitrogen) transfection into stable Tet-On-inducible E2a cells (E2T cells ...
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bioRxiv - Cancer Biology 2020Quote: ... the lentiviral plasmids and packaging plasmids were transfected into lentiX-293T cells using Lipofectamine 2000 (Invitrogen, Carlsbud, CA) and lentivirus-containing supernatants were harvested after 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were then extracted and purified using the PureLink HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific, Cat# K210006) for downstream experiments ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 µL of plasmid-lipofectamine mixture containing 75 ng plasmid and 165 ng Lipofectamine 2000 (Invitrogen, 11668-019) in Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid transfections were performed using 2 μg plasmid per 3.5-cm well plates using Lipofectamine 2000 (Life Technologies) transfection reagent according to the manufacturer’s recommendations at 48 hours before harvest ...
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bioRxiv - Genomics 2023Quote: ... Colonies were selected and plasmid DNA was extracted using The PureLink™ Quick Plasmid Miniprep Kit (Thermofisher Scientific) and sent for Sanger sequencing to identify the integrity and quality of the inserted enhancer fragment ...
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bioRxiv - Systems Biology 2023Quote: ... coli (High Efficiency) cells and the plasmid DNA was purified using the PureLink™ Plasmid Purification Kit (Invitrogen). Three oligonucleotide pools were cloned separately and pooled together to acquire the final 7mer library ...