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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Dengue Virus Serotype 1 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg protein for each condition was incubated with antibody-coated Dynabeads Protein G (Invitrogen, 1003D). Antibody dilutions are provided in table S3 ...
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bioRxiv - Biophysics 2022Quote: ... 1 μg of the dialyzed protein sample was mixed with Protein Thermal Shift Dye (Applied Biosystems) and 0.003 − 10 mM NaCl in 20 μL of buffer A ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cell Biology 2019Quote: ... The RNAi libraries used were a custom library targeting gene products predicted to localize to the nuclear envelope (346 genes, ThermoFisher, lot# AMO20JUZ), and an off the shelf library targeting proteins involved in epigenetic and chromatin regulation (521 genes ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 million HEK 293FT cells were seeded and transfected after 24 hours with the LentiCRISPRV2-IL11g1 or LentiCRISPRV2-IL11g2 construct together with the pLp Δ8,9 packaging plasmid and VSVG envelope plasmid using lipofectamine™ 2000 transfection reagent (Life Technologies, 11668019) and Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... and envelope plasmids into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher Scientific, MA, USA). Cell supernatant containing lentivirus was collected at 24 and 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Rabbit anti-Myelin Basic Protein (BMP) (ThermoFisher; 1:300), Mouse anti-NeuN (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized with 647 Phalloidin (1:100, Invitrogen), Cy3-Donkey anti-Rabbit (1:750 ...
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bioRxiv - Neuroscience 2020Quote: ... 1:100 anti-GNAL (Golfα protein (PA5-50981, Invitrogen), 1:100 anti-Flotilin-1 (3253 ...
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bioRxiv - Neuroscience 2020Quote: ... postsynaptic density protein 95 (PSD-95; 1:2,000, ThermoFisher), GABAAR-gamma 2 (GABAAR ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Triton and incubated protein G Dynabeads (Thermo Fisher) coupled to HA antibodies for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: ... containing 1 mL of Protein G-sepharose resin (Invitrogen) to purify IgG ...
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bioRxiv - Cancer Biology 2022Quote: 1 µl Cas9 protein (5 µg/µl, Thermo Fisher) and 100 pM of synthetic guide RNA (Tspan8 guide sequence 5’ – 3’ ...
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bioRxiv - Cell Biology 2022Quote: ... corresponding proteins were expressed from modified pPROEX-1 (Invitrogen)-based plasmids as N-terminal 6 x His-TEV fusion proteins ...
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bioRxiv - Biophysics 2022Quote: ... 1 % BSA blocked protein G agarose (Thermo Scientific 20398) and anti-CD3ε (UCHT1 clone ...
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bioRxiv - Microbiology 2023Quote: ... 1:150 diluted Bacterial Protein Extraction Reagent (Thermo Fisher); and 1 mg mL-1 of 2-nitrophenyl-β-galactopyranoside (Sigma)) ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was pre-cleared with 1:1 protein A:protein G Dynabeads (Life Technologies) and immunoprecipitation was carried out with H3K27ac-bead complexes at 4°C overnight ...
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bioRxiv - Genetics 2020Quote: ... 50 uL of a 1:1 mix of Protein A (Cat #10008D, Invitrogen) and Protein G Dynabeads magnetic beads (Cat #10004D ...
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cDNA were synthesized from 100 pg of RNA and quantified using the TaqMan® Fast Virus 1-Step Master Mix (Thermofisher, Waltham, Massachusetts, USA) following manufacter’s instructions with appropriate oligonucleotides and Taqman probes (Table S1) ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was subjected to one-step RTqPCR analysis as described using primers specific for the viral genome encoding for the RNA-dependent RNA polymerase (RdRp)23 and for E24 genes with TaqMan fast virus 1-step master mix (ThermoFisher Scientific, catalogue number 4444432) using AriaMx instrumentation (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was directly subjected to one-step RT-qPCR analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA) and Bio-Rad CFX instrumentation (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... The supernatant chromatin was pre-cleared with 10 μl of 1:1 protein A: protein G Dynabeads (Thermo Fisher Scientific, 10001D and 10003D). The precleared chromatin sample was incubated with 0.5 μg of anti-H3K4me3 (Millipore 17-614 ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Molecular Biology 2020Quote: ... and the virus was produced in HEK293A cells as described by the manufacturer (Invitrogen). Immature brown adipocytes were treated with the viruses on day 2.
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... then incubated with the appropriate amount of virus diluted in Fluorobrite DMEM (Life Technologies) medium supplemented with 2% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: For Sendai virus iPSC reprogramming using CytoTune™-iPS 2.0 Sendai Reprogramming kit (Invitrogen), fibroblasts were reprogrammed as previously described (Milagre et al. ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus stocks was purified using TRIzol Reagent (ThermoFisher Scientific, catalog no. 15596026). Brief ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs were synthesized using random primers and Moloney murine leukemia virus RT (Invitrogen). The RT-PCR products were separated on a 2% w/v agarose-TBE gel ...
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bioRxiv - Neuroscience 2020Quote: ... Fibroblasts were reprogrammed into iPSCs by using the replication-incompetent Sendai virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolated RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo(dT ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from mock or virus infected cells using Trizol reagent (Invitrogen) at 2 and 4 hours post infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... UKEi001-A was reprogrammed by a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). EHT patch casting was performed by mixing CMs and a fibrinogen/thrombin mix3 ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of virus stocks in Minimum Essential Media MEM medium (Gibco, #41500-018) supplemented with 2% FBS was added to Vero E6 monolayers on 24-well plates (Greiner bio-one ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... virus particles were then purified by gel filtration (7K molecular weight cut-off, Invitrogen).
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bioRxiv - Immunology 2021Quote: ... total RNA was reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (Fisher Scientific) using random hexamers (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... prior to addition of PE243 virus in complete DMEM media +10 mM HEPES (Gibco), at a multiplicity of infection (MOI ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: UKEi001-A was reprogrammed with a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). Expansion and cardiac differentiation of iPSC were performed as recently described1 ...
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bioRxiv - Neuroscience 2022Quote: ... Virus (undiluted) or FluoSpheres (580/605, 0.04 µm, diluted to 20% in saline, Invitrogen) were loaded into freshly pulled glass micropipettes and injected using a Nanoject III pressure injector (Drummond Scientific Company ...
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bioRxiv - Microbiology 2022Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Virus amplification was carried out in Spodoptera frugiperda Sf9 cells (Geneart, Thermo Fisher Scientific) and was used to infect Trichoplusia ni Tnao38 cells (103 ...