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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... and CMG2-GST was affinity purified with Glutathione Superflow Agarose (Thermo Fisher). Glutathione was removed and CMG2 exchanged into HBS-T with 50% glycerol using Sephadex G50 (GE Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with 200μl of GST-Sepharose beads (Thermo Scientific) and washed 6 times with CHAPS buffer containing 0.5 M NaCl and 3 times with CHAPS buffer containing 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μl (0.21 μg) of PKR(252-551)-GST enzyme (Thermo Scientific) was added ...
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bioRxiv - Biochemistry 2021Quote: ... The filtered lysate was then added to GST-beads (Thermo Fisher Scientific) pre-equilibrated in binding buffer and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST proteins were captured on Pierce glutathione magnetic beads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... except for passage of the final prep through a GST column (ThermoFisher). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing GST-Cdc6 were treated with 200 U PreScission protease (ThermoFisher) for 2 h on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Microbiology 2020Quote: ... The vector only encoding GST was transformed into BL21 DE3 (ThermoFisher Scientific). Plasmids encoding GST-YidC1CT or GST-YidC2CT were transformed into BL21 Star™ (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... was incubated with purified GST-tagged IKKi (catalog # PV4875; Thermo Fisher Scientific) in buffer A containing 20 mM Tris [pH 7.5] ...
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bioRxiv - Cancer Biology 2023Quote: ... GST-ISG15 was expressed in Escherichia coli BL21(DE3) (Thermo Fisher, EC0114) at OD600 0.6-0.8 with 0.1 mM IPTG and 20°C and lysed in the lysis buffer by sonication ...
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bioRxiv - Cancer Biology 2022Quote: ... bacterially expressed GST-p21 bound to glutathione Sepharose beads (Thermo Scientific, 16100) were incubated with His-RanBPM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Then GST was removed using 20 U/mg protein of Actev (Invitrogen) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... To detect PI(4,5)P2 a GST Tag Antibody (MA4-004; Invitrogen) was used ...
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bioRxiv - Molecular Biology 2021Quote: 10,000 HEK293 FRT were seeded in 96 well plates (M33089, ThermoFisher UK) and induced with tetracycline for 24 hours ...
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bioRxiv - Genomics 2019Quote: Total RNA was prepared from harvested knockdown HEK293 cells using TRIzol (ThermoFisher). Single-stranded DNA in the sense orientation was synthesized from total RNA with random priming using the GeneChip WT Amplification kit (Affymetrix) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T and HEK293 cells were cultured in DMEM 1X (Thermo Fisher Scientific) supplemented with 0.5 % Peniciline/Streptomycin 100X Solution (Biowest ...
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bioRxiv - Cell Biology 2019Quote: HeLa and HEK293 cell samples prepared in 6-well plates (Fisher Scientific) were used for the assay ...
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bioRxiv - Genetics 2019Quote: ... Plasmids were transfected into podocytes or HEK293 cells using Lipofectamine 2000 (Invitrogen). HEK293 cells stably expressing p3xFLAG-ADCK4 or BAP were selected and maintained with 1 mg/mL G418.
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bioRxiv - Neuroscience 2021Quote: HEK293 cell line were plated in 24-well plates in DMEM (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2021Quote: ... Ptk2 and HEK293 cells were incubated in imaging medium (FluoroBrite DMEM (Gibco) supplemented with 10 % FBS (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The tetracycline inducible cell line T-REx™-HEK293 (Thermo Fisher Scientific) was cultured under the following conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HEK293 cells using either Lipofectamine 2000 reagent (Invitrogen) or calcium phosphate transfection.
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bioRxiv - Cell Biology 2022Quote: NIH-3T3 (ATCC: CRL-1658) and HEK293-FT (HEK, ThermoFisher Scientific R70007) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 and S16 cells were cultured in DMEM (#41965, Thermofisher Scientific, USA) complemented with 10 % of FBS (#15000-036 ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells (ATCC) were maintained in DMEM (Gibco, Cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... The HEK293 derivate HEK293F cell line (Cat nr R79007, Thermo Fisher Scientific) were cultured in Freestyle 293 medium ...
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bioRxiv - Cell Biology 2022Quote: ... with a C-terminal 6x His tag was custom-synthesized by Invitrogen and transiently transfected in HEK293 T cells using polyethylenimine in OPTIMEM (Invitrogen) containing 2 mM CaCl2 ...
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bioRxiv - Genetics 2021Quote: ... The plasmids were used to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 suspension cells (growing in FreeStyle™ 293 Expression Medium, Gibco #12338) were transiently transfected with the vector coding for the scFv-Fc of each antibody ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells (ATCC) were cultured with Dulbecco’s Modified Eagle Medium (DMEM; Gibco) with 10% Fetal Bovine Serum (Biowest ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293S GnTI− cells were cultured in suspension in FreeStyle 293 medium (Gibco) supplemented with 2% fetal bovine serum ...
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bioRxiv - Genomics 2019Quote: ... lentiviruses were produced in HEK293 cells using ViraPower Lentiviral Expression System (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: A transgenic HEK293 containing GFP gene in the genome (ThermoFisher via MTA) was used in cell experiments ...
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bioRxiv - Molecular Biology 2019Quote: ... Huh7 and HEK293 cells were maintained in DMEM GlutaMAX (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Thermoscientific) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 GnTI- cells were grown and maintained in FreeStyle 293 medium (Gibco) supplemented with 2% fetal bovine serum ...
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bioRxiv - Molecular Biology 2020Quote: ... Splicing analysis was carried out in HEK293 cells after lipofectamine 2000 (Invitrogen) transfection of plasmids ...
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bioRxiv - Bioengineering 2022Quote: HEK293 based T-REx™293 cell line was obtained from Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were obtained from Thermo Fisher (Flp-In 293 TREx cells) and were cultured in DMEM with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... packaging vectors into HEK293-FT cells using Lipofectamine 2000 (Thermo Fisher Scientific). Viral supernatants at day 2 and 3 after transfection were collected ...
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bioRxiv - Genetics 2022Quote: ... HEK293 and COS-7 cells were transiently transfected using Lipofectamine 2000 (Invitrogen) or calcium phosphate (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells (ECACC 85120602) were maintained in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: The parental Jump-In™ HEK293 (Thermo Fisher Scientific, hereafter termed HEKR4) and all its derivatives were grown in medium containing DMEM (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Suspension HEK293S GnTI- cells were maintained in Freestyle media with GlutaMAX (Gibco) supplemented with 1% FBS and antibiotic-antimycotic solution ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 Ac2 cells were grown in DMEM supplemented with 10 % FBS (Gibco) and 4 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were cultured in a humidified Reach-In CO2 incubator (ThermoFisher) at 37 °C ...
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bioRxiv - Biophysics 2023Quote: Myosin-5 was purified from transiently transfected FreeStyle HEK293 cells (Thermo Fisher) as previously described (32) ...