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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution: Alexa 488-goat anti-rabbit (1:1000; Thermo Fisher Scientific) and Alexa 594-donkey anti-mouse (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
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bioRxiv - Neuroscience 2023Quote: ... DRG samples were incubated in the blocking solution with added primary antibodies: anti-GFP (Invitrogen, #11122, 1:400, 2 days, 37°C), and anti-V5 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C and total protein determined by the BCA method (Thermo Fisher Scientific). Proteins were separated on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... was carried out overnight at 4°C using Protein A Dynabeads (ThermoFisher Scientific). The mononucleosomal fraction (147 bp ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Bioengineering 2023Quote: ... protein expression was induced overnight at 16°C with 0.2mM IPTG (Thermofisher, 15529019). The overnight cultures were lysed and sonicated in non-denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and protein concentrations were quantified using a CBQCA Quantification kit (C-6667, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted by incubation at 95°C in NuPAGE Loading Buffer (Invitrogen) for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were expressed overnight at 18°C in Terrific Broth (Thermo Scientific) and 0.4% v/v glycerol with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitations were performed overnight at 4°C with Dynabeads Protein G beads (Invitrogen) conjugated to mouse anti-FLAG M2 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Molecular Biology 2019Quote: ... Immuno-precipitation was done overnight at 4°C using Protein A-coupled or Protein G-coupled Dynabeads previously incubated with anti-GFP A11122 antibody (Invitrogen), anti-Flag antibody (M2 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... solubilized tryptic peptides were quantified using the Pierce™ Quantitative Fluorometric Peptide Assay (ThermoFisher) as reported in previous work [17] ...
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bioRxiv - Microbiology 2021Quote: Labeled peptides were fractionated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific). The dried peptides were reconstituted and acidified with 0.1% TFA solution and loaded on the equilibrated ...
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bioRxiv - Cell Biology 2021Quote: ... Peptide quantitation was performed using Pierce Quantitative Colorimetric Peptide Assay Kit (Thermo Fisher, 23275) and 500 µg of each sample was retained for analysis by MS (pre-TAILS sample ...
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bioRxiv - Cell Biology 2022Quote: ... 2.Peptides were desalted using the Pierce™ Peptide Desalting Spin Columns (Thermo Scientific) according to the manufacture’s protocol no ...
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bioRxiv - Neuroscience 2019Quote: ... The peptide concentration was determined using the Pierce Quantitative colorimetric peptide assay (Thermo Fisher). The peptide concentration of the samples was adjusted to 140 μg/ml and analyzed using a Q Exactive HF-X mass spectrometer coupled to an Ultimate 3000 RSCLnano pump (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... we measured resulting peptide concentrations with the Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher, #90110); this step was not performed for the secretome samples ...
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bioRxiv - Systems Biology 2022Quote: ... The digested peptides were quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific), and stored at -80°C until the TMT labeling.
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bioRxiv - Neuroscience 2022Quote: ... Peptide samples were cleaned using Pierce™ Peptide Desalting Spin Columns (Cat#: 89852, ThermoFisher) and analyzed in duplicate by LC-MS/MS using a Thermo Easy nLC 1200-QExactive HF (Cat# ...
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bioRxiv - Neuroscience 2022Quote: ... and peptide concentrations were determined with Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific Inc.). TMT labeled sample LC-MS/MS acquisitions were performed using an EASY-nLC 1000 connected to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Neuroscience 2022Quote: ... Peptide concentrations were determined with the Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific Inc). A total of 10μg of peptide was prepared to label with TMTpro 16 plex reagents (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... Cross-linked peptides were desalted using Pierce peptide desalting spin column (Thermo Fisher Scientific) and dried ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were quantified using a Pierce Colorimetric Peptide Quantification Assay Kit (Thermo Scientific, 23275). 50 μg of each sample was separated for multiplex proteomic analysis ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: Final peptides were resuspended in 0.1% formic acid for peptide quantification (ThermoFisher Cat# 23275) and dilution to a final concentration of 0.25μg/μL ...
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bioRxiv - Systems Biology 2023Quote: Peptide concentrations were determined using the Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, 23290) and 18 µg peptides per time point were subjected to TMT labeling ...
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bioRxiv - Neuroscience 2023Quote: Peptides were quantified and normalized using a quantitative colorimetric peptide assay (Thermo Fisher Scientific). TMT reagents were reconstituted with 41 μL of anhydrous acetonitrile with occasional vortexing for 5 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Resulting peptides were desalted using Pierce™ peptide desalting spin columns (Thermo Fisher Scientific) according to manufacturer’s instructions and concentrated using a vacuum concentrator ...
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bioRxiv - Cell Biology 2023Quote: ... Peptide concentration of the supernatant was quantified using a quantitative colorimetric peptide assay (ThermoFisher). For TMT labelling we used 2 different kits to label each of the TMT experiments included in this manuscript ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptides were loaded onto a 0.3 x 5mm peptide trap column (ThermoFisher Scientific) at 30 μL per minute for 15 minutes before switching in line with a 0.075 × 250 mm nano easy column (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptide concentration was measured using the Pierce™ Quantitative Fluorometric Peptide Assay (ThermoFisher: 23290) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The peptide concentration was determined using the Pierce™ Quantitative Peptide Assay (Thermo Scientific). Aliquots of 30 µg peptides were labelled using TMTpro 18-plex isobaric mass tagging reagents (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The peptide concentration was measured by Pierce™ Quantitative Fluorometric Peptide Assay (ThermoFisher Scientific) in Cary Eclipse Fluorescence Spectrometer (Agilent ...
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bioRxiv - Immunology 2019Quote: ... MLN and whole blood cells were stained for live/dead with blue reactive dye (catalog#L23105, Invitrogen) and cell surface markers were 2 labeled with the following antibody panels ...
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bioRxiv - Immunology 2020Quote: ... Fluorescent labeling of antibodies was done with DyLight 488 or DyLight 647 Amine-Reactive Dye (Thermo Scientific) according to manufacturer’s instructions ...