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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 7H Benzocyclohepten 7 one 2 amino 5 6 8 9 tetrahydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... was co-transfected with either pre-gRNA or gRNA expression plasmids (1 µg per 6-well) using Lipofectamine 3000 kit (5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were first transfected with the pcDNA3_CasRx-GFP plasmid (2.5 µg per 6-well) using Lipofectamine 3000 kit (5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2020Quote: ... and then 10 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Life Technologies) was added to the cells and they were incubated for one hour ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... One mL Texas red dextran solution (2 mg/mL; Invitrogen) was injected into the left ventricle at an even speed for 1 minute ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 5% FBS and 0.1 mM MEM non-essential amino acids (NEAA; Gibco) for 48 h ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 5% FBS and 1X non-essential amino acid solution (NEAA) (Gibco, 11140050). The next day ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL non-essential amino acids (NEAA; 1X final concentration, Thermo Fisher Scientific, 11140050), 500 μL β-mercaptoethanol (21985-023) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL non-essential amino acids (NEAA; 1X final concentration, Thermo Fisher Scientific, 11140050).
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bioRxiv - Biochemistry 2020Quote: ... was mixed with 5/6 TAMRA-OSu (Thermofisher #C1171) in DMSO ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 for 6 days in RPMI (Life Technologies) supplemented with [5%] AB serum ...
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bioRxiv - Biochemistry 2024Quote: ... NHS-5/6-carboxyfluorescein (NHS-fluorescein, Thermo Fisher 46410), NHS-Oregon Green (Thermo Fisher O6147) ...
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bioRxiv - Cell Biology 2020Quote: ... 6-ketocholestanol or phloretin cells were incubated with di-8-ANEPPS (Thermo Fisher, D3167) at a final concentration of 2 μM on ice for 20 minutes (33 ...
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bioRxiv - Developmental Biology 2021Quote: ... 6 and 8 hours using an EVOS FL Cell Imaging system (Thermo Fisher Scientific). Each experiment was performed on 5-6 litters for every treatment ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 6-well plates in Essential 8™ Basal Medium (E8; Gibco; A15169-01) supplemented with Antibiotic-Antimycotic (100x ...
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bioRxiv - Microbiology 2020Quote: ... 5 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific) were applied for monitoring effector caspase activation and 2.5 µM AlexaFluor647 hydrazide for detecting cell lysis.
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bioRxiv - Cancer Biology 2022Quote: ... 5 and 7 days in culture by staining with trypan blue (Invitrogen) or counting with an automated cell counter for viable cells (Fluidlab R-300 cell counter ...
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bioRxiv - Developmental Biology 2020Quote: ... for 5-7 minutes before quenching with Knock-Out Media (ThermoFisher Scientific) and centrifuging at 200g for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 5-7 µL of NativeMark Unstained Protein standard from Invitrogen (LC0725) was used as the ladder ...
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bioRxiv - Microbiology 2023Quote: ... one primer was 5’ phosphorylated using a T4 Polynucleotide Kinase (ThermoFisher) using recommended reaction conditions ...
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bioRxiv - Biochemistry 2021Quote: ... Freshly prepared, ice-cold RIPA buffer (110 μL, 50mM Tris, pH 7-8 (Acros Organics #14050-0010), 150 mM NaCl (Fluka #71383) ...
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bioRxiv - Immunology 2024Quote: ... Frozen lungs were cut into 7-8 µm sections using a Cryostar NX50 cryostat (Thermo Fisher Scientific) and mounted on silane-treated glass slides (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... which was previously labeled at its 5’ end with one of four specific fluorescent dyes (6-FAM®, NED®, PETTM and VICTM; Thermo Fisher Scientific, Waltham, MA, U.S.A) (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (5 µM, Gibco) and 150 IU/ml human rIL-2 and 50ng/ml rIL-15) ...
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bioRxiv - Neuroscience 2021Quote: DRGN and retinal cells were washed with PBS and total RNA (n = 6-9 wells/treatment) was extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen). Appropriate primers were used to validate each gene of interest using the specific primers (Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Neuroscience 2020Quote: ... 8 µL 2 mM dNTPs (Thermo Fisher Scientific cat# R0241), 6.4 µL 10 µM 5’ 6-FAM-labelled CAG1 primer 34 ...
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bioRxiv - Molecular Biology 2019Quote: ... 15N2 L-lysine-2 HCl (Lys-8) (Thermo Fisher Scientific). Cells were kept in a humidified atmosphere with 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... and Di-8-ANEPPS (Invitrogen™, cat. D3167, 2 µM) at 5% CO2 at 37 °C for 15-20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 15N2 L-lysine-2 HCl (Lys-8) (Thermo Fisher Scientific). To avoid contamination of light amino acids ...
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bioRxiv - Neuroscience 2023Quote: For lightsheet experiments 6-7 dpf fish are placed in 2.2% low-melting point agarose (Thermofisher) in a chamber optimized for our lightsheet microscope.
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using the QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). The setting of the cycles used was the default for comparative Ct studies ...
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bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... adding 2 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) and 1 μM QUMA-1 23 to the final wash ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Biochemistry 2019Quote: The N30 Random DNA pool and cDNA from rounds 7-to-9 were cloned into TOPO™ TA Cloning™ Kit (Invitrogen) following manufacturer’s specifications ...