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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 6 OXABICYCLO 3.2.1 OCT 3 EN 7 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 × 106 HEK293F cells were transfected with one of these constructs using 293fectin Transfection Reagent (Thermo Fisher) or 293-Free (EMD Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... lungs were filled with 80% Tissue Plus OCT Compound (Fisher Scientific, Hampton, NH)/20% saline solution ...
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bioRxiv - Bioengineering 2022Quote: OCT sections were permeabilized for 15 minutes with 1% Triton X (Thermo Fisher) and blocked for 2 hours with a solution containing 0.5% TritonX-100 and 5% donkey serum (Thermo Fisher) ...
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bioRxiv - Bioengineering 2020Quote: ... 3D renderings of OCT images were performed using Amira software (Thermo Fisher Scientific).
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bioRxiv - Immunology 2019Quote: ... single intestinal biopsies were embedded in OCT cryo-embedding matrix (Thermo Fisher Scientific) then frozen in isopentane (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cryopreserved sciatic nerves were embedded in Optimum Cutting Temperature (OCT) compound (ThermoFisher Scientific), frozen and cut longitudinally (Cryostat Leica CM3050S ...
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bioRxiv - Cell Biology 2021Quote: ... mouse tissue samples were embedded in optimal cutting temperature compound (OCT, Life Technologies) prior to sectioning ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then embedded in OCT (optimal cutting temperature) compound (Thermo Fisher Scientific). 25 µm sections were serially collected in both coronal and sagittal planes using a Reichert Frigocut Cryostat E-2800 ...
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bioRxiv - Bioengineering 2023Quote: ... Brains were embedded in optimal cutting temperature (OCT, Thermo Fisher, #23-730-571) media ...
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bioRxiv - Physiology 2023Quote: ... in Sakura Finetek Tissue-Tek optimal cutting temperature compound (OCT; Thermo Fisher Scientific), and plunge-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Dermal substrates were subsequently embedded in optimal cutting temperature compound (OCT, Life Technologies) and frozen before sectioning and staining with haematoxylin and eosin ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Immunology 2020Quote: ... from each sample was reverse-transcribed using the qScript™ One-Step SYBR® Green qRT-PCR kit in a ViiA-7 RT-PCR system (Thermo Fisher Scientific). The GAPDH gene was used as the endogenous control ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Physiology 2023Quote: ... For cell viability assays 7-AAD (7-Aminoactinomycin D, Invitrogen, A1310) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... ZIP13K2-derived EN cells (60 x 106) were harvested and cross-linked in 1% formaldehyde (Thermo Fisher Scientific, 28908) in DPBS for 5 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleus staining was performed using 4’,6-diamidino-2-phenylindole (DAPI) (3 mM, D3571, Molecular Probes). Cells were counted from four randomly selected fields per culture under a confocal microscope (TCS SP8 ...
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bioRxiv - Cell Biology 2022Quote: ... 3-6 dpf larval zebrafish were anesthetized using 0.2 mg/mL Tricaine-S (Fisher Scientific, NC0872873) solution ...
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bioRxiv - Biochemistry 2021Quote: ... 2-[6-(4’-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) from Molecular Probes® and Horse radish peroxidase (HRP ...
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bioRxiv - Physiology 2021Quote: 2-3 viable human slices were incubated with Fluo4-AM (6 μM, Invitrogen cat. No. F1221) for 1h in 3 mM HEPES buffer (125 mmol/l NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... 3-[p-(6-phenyl)-1,3,5-hexatrienyl] phenylpropionic acid was purchased from Molecular Probes (Eugene, OR, USA) and 1-stearoyl-2-linoleoyl-sn-glycerol-3-phosphocholine (SLPC ...
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bioRxiv - Biochemistry 2023Quote: ... batches of 3-6 SC-islets were embedded in extracellular matrix (Geltrex, Gibco, cat. n A1569601) and cultured for 7 days under perfusion ...
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bioRxiv - Cell Biology 2020Quote: ... The mEGFP-tagged Tom20 line was transfected with mCherry-Mito-7 construct (Michael Davidson, addgene #55102) using 6 μl per well of transfection mixture containing 25 μl Opti-MEM (ThermoFisher #31985-070), 1.5 μl GeneJuice (Millipore #70967 ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Immunology 2020Quote: ... or aged (26-28 mo) female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco, 11995-073) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were seeded into 6-well plates (one million per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 25 mM unlabeled glucose ...
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bioRxiv - Genomics 2020Quote: ... 10 ng of the library were transfected into 250 000 HEK293 cells in one well of a 6-well plate using Lipofectamine 2000 (11668027, ThermoFisher Scientific) and OPTIMEM I Reduced Serum Medium (31985-047 ...
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bioRxiv - Cell Biology 2021Quote: ... One microgram of siRNA was transfected in one well of a 6-well using Lipofectamine RNAiMAX transfection reagent according to the manufacturer protocol (Thermofisher Scientific). Cells were treated with siRNA for 24h.
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bioRxiv - Genetics 2023Quote: Whole cell extracts of 2D cultures from one well of a 6-well plate were prepared using RIPA Lysis and Extraction Buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Immunology 2023Quote: ... Monocyte-derived macrophages (MDM) were generated by seeding three million CD14+ cells into one 6-well plate (NuncTM Thermo Fisher Scientific) in IMDM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Aggregates from four wells of a 6-well plate were transferred to one 175 cm2 tissue culture flask (Thermo Scientific, #159926) previously treated with an anti-adherence solution (StemCell Technologies ...
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bioRxiv - Microbiology 2023Quote: All transfections were performed on 293T cells (seeded at a density of 0.5 × 106 cells/well of a 6-well plate one day prior) using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendation unless stated otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was quantified using a Nanodrop One-One (Thermofisher) before cDNA amplification or RNA seq libaries preparation.
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed by measuring caspase 3 cleavage using a CellEvent Caspase3/7 Green Flow Cytometry kit (Thermo Fisher), according to the manufacturer’s descriptions ...
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bioRxiv - Cell Biology 2021Quote: ... The tissues were then digested in room temperature for 7 min in a 3-mL Hank’s solution containing 0.25% Trypsin (Thermo Fisher, 15090046) and 0.1 μg/μL DNase (Sigma D5025 ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed using SYBR green reagents on an Applied Biosystems QuantStudio 3 and ViiA 7 Real-Time PCR Systems (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... FACS analysis of transfected BEAS-2B cells for caspase 3 & caspase 7 along with SYTOX AADvancedTM Dead Cell Stain was performed per manufacturer’s protocol (Thermo Scientific).
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bioRxiv - Physiology 2021Quote: ... JC-1 dye was added 10 min prior to recording (ex.488/em.535 and em.590) while CellEvent Caspase 3/7 (ThermoFisher) was added immediately prior to recording (ex.495/em.540).
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...