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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 4 Bromo 4' Butyl 1 1' Biphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The samples were fixed for 1 hour using 4% paraformaldehyde (PFA, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mm thick Bolt Bis-Tris Plus 4-12% gradient polyacrylamide gels (Invitrogen NW04125) in MOPS buffer at 200 V for approximately 30 min ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 4:3:1 using Lipofectamine 3000 kit (Thermo Fisher Scientific). Viral supernatant was filtered ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI) (1 µg/mL, Invitrogen), and actin with Alexa FluorTM 647/546-labelled phalloidin (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... slices were fixed with 4% paraformaldehyde at 4°C overnight and incubated with Alexa 647 coupled-streptavidin (Invitrogen S21374, 1:500 in PBS-10% tween) for 6 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Zebrafish fins or larvae euthanized in 1% Mesab were fixed in 4%PFA/1xPBS and embedded in 1% agarose (CryoStar NX50, Thermofisher) or tissue freezing medium (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated overnight at 4°C with the following primary antibodies at a concentration of 1:500: mouse anti-V5 (1:500, Invitrogen), rabbit anti-γ-H2AX (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... (iii) incubated overnight at 4 °C in the following affinity-purified rabbit KV7.2 (residues 1–70; 1:1000, Thermo Scientific), KV7.3 (residues 668–686 ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 mL of cold 1 X PBS (4°C) containing protease inhibitor (ThermoFisher; cat# 78429) and were transferred into tubes containing lysing matrix B (MP Biomedicals ...
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bioRxiv - Developmental Biology 2022Quote: ... with 0.1% (w/v) Tween20 and incubated overnight with primary antibodies at 4° C: anti-HIF1α (1:500, MA1-16504 Invitrogen); anti-STAT3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS containing 0.3 % Triton X-100 and incubated overnight at 4°C with a rabbit anti-Iba1 antibody (1:500, Wako) in PBS containing 1 % FBS (Gibco), 0.1 % BSA (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Blot sections were probed overnight at 4°C 1:5000 with the ATXN1 antibody 11750 (Servadio et al., 1995) or 1:2500 with H1 antibody (Invitrogen) diluted in 5% milk PBST ...
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bioRxiv - Microbiology 2021Quote: ... clarified transfection supernatant was diluted 1/4 in a 20 μl volume of transfer buffer (Life Technologies Cat# NP0006-1) and applied dropwise to a presoaked 0.45 μm PVDF membrane ...
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bioRxiv - Biophysics 2021Quote: ... or ParB-EDTA buffer (by replacing the 4 mM MgCl2 with 1 mM EDTA) and then incubated with 1 μm diameter magnetic beads (Dynabeads, Myone streptavidin, Invitrogen) for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Pathology 2020Quote: ... The aqueous phase was separated by centrifugation (21,000 g for 15 min at 4 °C) and incubated with isopropanol (1:1 ratio) and 25 μg of Glycoblue (ThermoFisher) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked in 5% normal goat serum + 2% BSA and 2% Triton-X for 1 h and incubated with the following primary antibodies overnight at 4°C: rabbit anti-GFP (1:1000, Invitrogen) and chicken anti-TH (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at 30V for 1 hour at 4°C in 1X NuPAGE™ buffer (REF NP0006-1, Life Technologies). After transfer ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Loss of function of NEU1-4 was conducted by transfecting cells with pooled siRNAs (Supplemental Table 1) (Dharmacon) or single siRNAs (Supplemental Table 1) (ThermoFisher) using RNAiMAX (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged once a week (∼80% confluence/well) at 1:4-1:6 ratios using 1mg/ml collagenase (Gibco) to detach colonies and onto fresh ir-MEF plates.
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bioRxiv - Physiology 2022Quote: ... Cell surface staining was done in live cells blocked with diluted superblock (1:4 dilution in PBS with 1 mM MgCl2 and 0.1 mM CaCl2, ThermoFisher Scientific) at 4°C for 0.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... mono-Methylated K35 overnight (1:100) at 4°C followed by 1:100 Alexa-Fluor 488 and Alexa-Fluor 555 (both Invitrogen) for 2 h at RT.
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bioRxiv - Cancer Biology 2024Quote: ... the Live/Dead assay was conducted using 1 μM calcein AM and 4 μM ethidium homodimer-1 (Life Technologies kit) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 45 minutes with 1:500 AlexaFluor 488-conjugated anti-mouse (Invitrogen, 1:500 in 4% BSA PBS) plus DAPI (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
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bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
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bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107, lot GR3234832-4; Donkey anti-Rabbit 594nm 1:1000, Invitrogen A21207 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated overnight at 4 °C with the following antibody: ERα (Dilution 1:1 µg/mL; Invitrogen, CAT: MA5-13191). IRDye secondary antibody (Dilution 1:20,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Media was partially renewed every 3-4 days with neuronal differentiation media 2 (NDM2: 1:1 DMEM/F12:NB (Gibco), glutamax (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000 ...
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bioRxiv - Immunology 2023Quote: ... The upper aqueous layer was removed to a new tube and mixed 1:1 with Isopropanol (Fisher Scientific, BP2618-4) and incubated for 1 min with glycoblue co-precipitant (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1.2 M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 µg/mL DAPI (4’, 6-diamidino2-phenylindole; Molecular Probes). Cells were imaged with a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Microbiology 2024Quote: ... Two skin swab samples were taken in a 5 mL centrifuge tube and mixed with 1 mL of pre-cooled methanol-water (V:V=4:1, Thermo Fisher), after brief sonication in ice water for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... they were dissociated with 0.5 mM EDTA in DPBS and split every 4-5 days at a ratio 1:8-1:12 using Revitacell Supplement (Gibco). Fibroblasts and early passage iPSCs were cultured at 37°C in 5% CO2 and frozen at passage 4-5.
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bioRxiv - Systems Biology 2024Quote: ... the membranes were then incubated overnight at 4°C with either: (i) rabbit anti-TOM20 (1:5000, 11802-1-AP, ThermoFisher); (ii ...
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bioRxiv - Immunology 2024Quote: ... while A/Darwin/6/2021 full-length soluble trimer and A/Hong Kong/1/1968 head trimer (see below) were mixed in 4:1 molar ratios with SA phycoerythrin (PE; Invitrogen) and SA-Allophycocyanin (APC ...
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bioRxiv - Bioengineering 2024Quote: ... followed by an additional 20 minutes at room temperature after adding ethidium homodimer-1 (ETHD-1, 4 µM, Invitrogen E1169). Viability was quantified from 200 µm stacks acquired using a Leica DMi8 THUNDER microscope and reported as the ratio of live cells (Hoechst+/ETHD- ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA labeling was performed using PBS containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (1:10000) (Invitrogen, D1306). After 3 additional washes with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Biochemistry 2021Quote: ... Succinimidyl 6-(4, 4’-azipentanamido)hexanoate (LC-SDA; Thermo Scientific) crosslinking was performed by adding the reagent to final 1mM concentration ...