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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 2 Bromo 1 5 bromofuran 2 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Genetics 2020Quote: ... Cells were seeded in 6-well plates and transfected with 4 μg/well vectors expressing CasRx-GFP and gRNAs-mCherry (CasRx:gRNA-1:gRNA-2 = 2:1:1, see Supplementary sequences) using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Control group was only transfected with 2 μg/well vectors containing CasRx-GFP ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Zoology 2020Quote: ... 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-23491-52-3 (Hoechst 33342, trihydrochloride, trihydrate, Life Technologies, H3570) in water for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 2% FBS in HBSS containing 5 g/ml DAPI (Invitrogen), and sorted into DMEM containing 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin complemented with appropriate Artemisia infusion dilutions and with or with IPP and Cm ...
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bioRxiv - Cancer Biology 2020Quote: ... and 4’,6-diamidino-2-phenylindole at 5 µg/mL (DAPI; ThermoFisher) in TBS 1% BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... containing 4’,6-diamidino-2-phenylindole (DAPI, 5 µg/ml, Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... 2.5 mg of 5-ethynyl-2’ deoxyuridine (EdU) (Thermo Fisher Scientific, A10044) was injected intraperitoneally 12 h prior to euthanasia ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μl of 2 x Novex TBE-Urea Sample Buffer (ThermoFisher Scientific) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... for 4h and EdU (5-ethynyl-2’-deoxyuridine from Invitrogen; 10µM final) was added 1h before cells were harvested ...
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bioRxiv - Immunology 2020Quote: ... 50 μM 2-mercaptoethanol and 5% NCTC-109 medium (Gibco, Waltham, MA). For induction of SHM ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were injected intraperitoneally with 5-ethynyl-2’-deoxyuridine (EdU; E10187; Invitrogen) at 10 mg/kg 3 hours prior to euthanasia to assay cellular proliferation.
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bioRxiv - Physiology 2021Quote: ... The fibers were then loaded with 5 μM Fura-2 AM (Invitrogen) by incubating them for 20 min at 19°C in Tyrode’s buffer ...
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bioRxiv - Immunology 2021Quote: ... 5 μg/mL of brefeldin A and 2 μM Monensin (both ThermoFisher) were added to each well ...
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bioRxiv - Systems Biology 2023Quote: ... cells were incubation with 10uM EdU (5-ethynyl-2’ -deoxyuridine) (Invitrogen, C10424) for 48hours together with either recombinant-mouse TIMP1 protein (1µg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated with 400 µM 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen)/DMSO in fish water for 1 hour at 28°C ...
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bioRxiv - Developmental Biology 2023Quote: ... containing 2% fetal bovine serum and 5 mM HEPES buffer (Life Technologies). For live imaging experiments ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 μL was loaded into a 2% agarose gel (Thermo Fisher) in 1X TAE buffer (MP Biomedicals) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were pulsed with 10μM EdU (5-ethynyl-2-deoxyuridine, Invitrogen A10044) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: To detect DNA synthesis EdU (5-ethynyl-2′-deoxyuridine; 10 μM, Invitrogen) was added to the medium and incubated for 24 h from days 8 to 9 post-treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were resuspended in 2 μL HBSS (Gibco, Cat# 14170088) and stored on ice ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with 2 or 5 μM CellTrace Violet (Invitrogen, #C34557). For ex vivo reculture experiments ...
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bioRxiv - Microbiology 2023Quote: ... 2 μL of 5 X SYPRO orange (Life Technologies, Eugene, Oregon, USA) and 2.5 μL of the 20 mM ligand solution or the equivalent amount of buffer in the ligand-free control ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 nmoles of RNA were mixed with 5 µg yeast tRNA (Invitrogen) and SELEX buffer (SB ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of 5 X SYPRO orange (Life Technologies, Eugene, Oregon, USA) and 2.5 µl of the resuspended array compounds or the equivalent amount of buffer ...
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bioRxiv - Cell Biology 2024Quote: ... with EdU (5-ethynyl-2’-deoxyuridine) (A10044, Invitrogen™/ Thermo Fisher Scientific) as a DPBS solution 200ug/20g body weight ...
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bioRxiv - Cell Biology 2024Quote: ... with EdU (5-ethynyl-2’-deoxyuridine) (A10044, Invitrogen™/ Thermo Fisher Scientific) as a DPBS solution 200ug/20g body weight ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split at 1:2 every 2 days using Trypsin-EDTA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Biochemistry 2023Quote: The panel of synthetic peptide standards and the products of immunoprecipitated heparan-sulfate 6-O-sulfotransferase 1/2/3 (H6ST-1/2/3) in vitro Tyr sulfation assays were analyzed using Proteome Discoverer 2.4 (Thermo Scientific) in conjunction with MASCOT 59 against either a custom database of all (12 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...