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Citations for Thermo Fisher :
4401 - 4450 of 5129 citations for JIP3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: Peptide digests of purified RSVF and antibodies were analyzed in DIA mode on a Thermo Scientific Lumos mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... internal diameter 100 μm) for desalting and a C18 analytical column (length 10 cm, internal diameter 75 μm) for peptide separation (Thermo Scientific). Peptides were eluted with a three-step 45 min gradient of 0.1% formic acid in ACN at a flow rate of 300 nl/min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... The digested peptides were cleaned-up using the HiPPR detergent removal resin kit (PN 88305, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm × 2 cm PepMap100 C18 nano trap column and a 75 μm × 25cm ...
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bioRxiv - Cell Biology 2023Quote: The TMT-labelled peptide samples were analysed with an Easy-nLC1200 liquid chromatography system combined with Q Exactive HF mass spectrometer (Thermo Scientific) using a 136 min gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately one µg or less for each SEC fraction was injected and the peptides were separated on an Acclaim PepMap RSLC column (150 mm × 75 µm, 2 µm particle size, ThermoFisher Scientific) at a flow rate of 300 nl/min ...
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bioRxiv - Microbiology 2023Quote: ... Multiplexed TMT samples were separated into four fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). TMT labelled fractions were analysed in an Orbitrap Fusion Lumos using a resolution of 50,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides in each sample were labeled with one of the tags of TMTpro 18-plex labeling kit (A52045, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Both the digested peptides from non-enriched and enriched samples were labeled with 16-plex TMT according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2023Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Immunology 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Molecular Biology 2023Quote: ... peptides were desalted on StageTips (36) and analysed by nanoflow liquid chromatography on an EASY-nLC 1000 system (Thermo Fisher Scientific) coupled online to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermofisher Scientific) using a 180 min gradient ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting phosphopeptides and whole cell lysates (flow through) were fractionated using Pierce High pH Reverse-Phase Peptide Fractionation kit (Thermo Fisher). Samples were dried and reconstituted in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2023Quote: The LC-MS/MS analyses of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos Mass Spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG resin was washed with Trisbuffered saline (TBS) and eluted with 25 μL or 2 mg/mL 3X FLAG peptide (Fisher Scientific). Samples were analyzed by mass spectrometry as described below.
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bioRxiv - Biochemistry 2022Quote: ... The beads were bound to the magnet and the supernatant containing the peptides were extracted and dried in a SpeedVac (ThermoFisher Scientific). Samples were brought up in 50 μL of 100 mM TEAB and labeled with TMT10plex (LOT VG306772 ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were separated on an EASY-Spray column (25 cm × 75 μm ID, PepMap RSLC C18, 2 μm, Thermo Scientific) connected to an EASY-nLC 1000 nUPLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: All peptide samples were spiked in with iRT standard peptides (Biognosys AG, Schlieren, Switzerland) and applied to MS analysis using EasyLC nano HPLC (Thermo-Fisher Scientific), Vanquish Neo HPLC system (Thermo-Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18, 5 μm, 50 cm, 100 A° Easy nano spray column # ES903, Thermo Scientific) at a flow rate of 250 nl/minute flow rate by applying nonlinear gradient of solvent-B (80% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... A column for affinity purification was prepared by binding the peptide to a SulfoLink resin column using the SulfoLink Immobilization Kit (Thermo Fisher). Antibodies were affinity-purified from serum using this column.
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bioRxiv - Cell Biology 2022Quote: ... reversed phase liquid chromatographic separations of peptides were performed on a C18 EASY-spray and C18 trap columns connected to an Ultimate 3000 UPLC system (Thermo Scientific). Mass spectra were acquired on an Q Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Nanoscale liquid chromatography (LC) separation of tryptic peptides was performed on a Dionex Ultimate 3000 Rapid Separation LC system (Thermo Fisher). Peptides were loaded onto a 20 μL nanoViper sample loop (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were separated on a C18 Acclaim PepMap nanoLC column (75 μm x 50 cm nanoViper, PN 164942, Thermo Fisher Scientific) equilibrated with 4% solvent B (99.9% acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides were separated by means of nanoscale C18 reverse-phase Liquid Chromatography (LC) using an EASY-nLC II 1200 (Thermo Scientific) system directly coupled to a mass spectrometer (Orbitrap Fusion Lumos ...
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bioRxiv - Biochemistry 2023Quote: ... The malonylated synthetic peptide TVDGPSGKmaLWR* containing a stable isotope-labeled arginine residue (R* with 13C6 and 15N4) was obtained from Thermo Fisher Scientific at >90% chemical purity and >99% isotopic purity ...
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bioRxiv - Systems Biology 2023Quote: ... Peptides were enriched using a C18 precolumn and separated on a 50 cm analytical column (EASY-Spray Columns, Thermo Fisher Scientific) at 50°C using a 265 min gradient ranging from 0 to 40% acetonitrile/0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of non-phospho or phospho-CTD peptide (per condition) was conjugated to 10 µL (per condition) high-capacity streptavidin agarose beads (Cat#20357, Thermo Scientific) in peptide binding buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Liquid chromatography (Ultimate 3000 nanoLC system) was used to fractionate the peptides online and then analyzed via an Exploris480 mass spectrometer (Thermo Fisher). MudPIT runs were carried out by 10µL sequential injections of 0 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm × 100 μm ID, 5 μm, 100 Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Physiology 2023Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC system (U3000-RSLC ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were were analyzed with a nanoAcquity UPLC system (Waters) coupled with Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher), with a procedure published previously (20) ...
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bioRxiv - Cell Biology 2023Quote: ... Phospho-peptides were enriched in these samples using High-Select™ Fe-NTA Phosphopeptide Enrichment Kit according to manufacturer’s protocol (ThermoFisher Scientific). Phosphopeptides were resuspended in 133 mM HEPES (SIGMA ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on an EASY-Spray PepMap RSLC C18 column (500 mm × 75 μm, 2 μm particle size, Thermo Scientific) over a 60-minute gradient of 2-35% acetonitrile in 5% dimethyl sulfoxide ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a nano-HPLC (Ultimate 3000, Dionex) with an inverse phase C18 Pepmap® column (Dionex, Thermo Fisher), following a gradient of 100% solution A (0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were first trapped on a C18 column (75-μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Fisher Scientific) with buffer A (2:98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... The flow through fraction was then fractionated into 13 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher).
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bioRxiv - Bioengineering 2023Quote: ... 2 µg of digested peptides were loaded into mass spectrometry system (Q Exactive Hybrid Quadrupole-Orbitrap MS, Thermo Fisher Scientific, USA). Hypersil gold C18 (100×2.1 mm ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of peptides for label-free LC/MS analyses were loaded to an Ultimate 3000 UHPLC/Q-Exactive system (Thermo Scientific) with an Acclaim PepMap RSLC 75 μm × 15 cm nanoViper column and run for 1 h.
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bioRxiv - Molecular Biology 2023Quote: ... A 2 μg aliquot of the peptide mixture were analysed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific ...