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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µl of 100 µM bottom splint (IDT) diluted in 7 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The reaction was annealed at 95 °C for 1m following 20 s at 95 °C and decreasing temperature by 1 °C every cycle ...
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bioRxiv - Immunology 2021Quote: Human T cells were differentiated for 6–7 days in a humidified 5% CO2 incubator at 37°C with IMDM (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: ... was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies, Carlsbad, Calif, USA). For TFH cell sorting ...
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bioRxiv - Cell Biology 2019Quote: 1.5 cm-thick intact human brain slice (Number 7, see Fig. S3) was randomly chosen and passively incubated with 400 ml TO-PRO-3 (T3605, Thermo Fisher, 1:2000 dilution) in PBS at room temperature for 1 week ...
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bioRxiv - Bioengineering 2021Quote: ... Cell nuclei were stained with 10 mM Hoechst 33342 for 7 min followed by mounting using ProLong™ Gold Antifade (ThermoFisher, Waltham, MA, USA). Images were taken using an Olympus FV 1000 confocal microscope.
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bioRxiv - Cancer Biology 2020Quote: ... Transduction of MCF-7 cells and selection of stably transfected clones were performed according to the ViraPower™ Lentiviral Expression Systems user manual (Invitrogen, 25-0501 MAN0000273).
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Pathology 2022Quote: Cell viability of the MRC-5 cells cultured on LdECM and Ru-LdECM hydrogels was assessed after 1 and 7 days using Calcein AM (Thermo Scientific, Breda, the Netherlands) to stain live cells and propidium iodide (PI ...
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bioRxiv - Cell Biology 2021Quote: ... The Capella FIB column provided higher practical milling currents up to 30 nA compared to 7 nA of the Magnum FIB column (Thermo Fisher Scientific, previously FEI) used in our prior work5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and COS-7 cells (ATCC® CRL-1651™) were maintained in complete medium (DMEM supplemented with 10% fetal bovine serum, both from Thermo Fisher Scientific) and grown in a standard incubator at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 7-9 hypervariable region of 16S rRNA following the manufacturer’s instructions performed using Veriti™ 96-Well Thermal Cycler (Thermo Fisher Scientific, Waltham, MA). A 2 % Agarose gel (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... of the paraffin-embedded spinal cord tissue blocks were cut at a thickness of 6-7 μm using semi-automatic motorized microtome (HM 355, Thermo Fisher Scientific, Kalamazoo, MI). The sections were stained Cresyl Violet (Nissl stain ...
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bioRxiv - Physiology 2020Quote: Bone biopsies stored at -80°C between 1 and 7 days were placed into liquid nitrogen contained in a ceramic mortar sprayed with RNaseZap® (ThermoFisher Scientfic, MA, USA). Samples were pulverised using an RNaseZap-treated pestle ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated at 37 °C for 7 days with secondary Alexa Fluor 647 donkey anti-rabbit IgG (1:450; Thermo Fisher Scientific, Rockford, IL) in 3% donkey serum/PTwH and then washed in PTwH for 5 times.
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bioRxiv - Microbiology 2019Quote: ... Real-time PCR was conducted with the Applied Biosystems PowerUp SYBR Green Master Mix on the Applied Biosystems ViiA 7 Real-Time PCR system (Thermo Fisher Scientific, Waltham, M.A.), using the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by dissociation with Accutase™ (37°C, 5 mins) and thereafter FACs sorted for viable cells using 7-AAD staining (Thermo Fisher Scientific, #A1310).
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bioRxiv - Evolutionary Biology 2021Quote: ... Fecal samples were immediately transferred to an anaerobic chamber within 2 h after defecation and suspended at 20% (w/v) in reduced peptone water (0.1%, pH 7; Thermo Fisher Diagnostics AG, Pratteln, Switzerland). Immobilization of 10 ml fecal slurry in polymer gel beads (gellan gum (2.5% ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Immunology 2020Quote: ... from each sample was reverse-transcribed using the qScript™ One-Step SYBR® Green qRT-PCR kit in a ViiA-7 RT-PCR system (Thermo Fisher Scientific). The GAPDH gene was used as the endogenous control ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell number assessed by DNA content was determined after 7-10 days of culture using CyQUANT Direct Cell Proliferation Assay (Life Technologies, #C35011, Carlsbad, CA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and gene-specific primers were used to perform quantitative reverse transcription-PCR (qRT-PCR) on 5 ng/μL cDNA in a Real-Time PCR System (Applied Biosystems ViiA 7, USA). Table S2 lists the primers used in qRT-PCR ...
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bioRxiv - Physiology 2023Quote: ... Transverse 7 μm-thick frozen sections were cut with a Leica cryostat and mounted onto SuperFrost Plus slides (Thermo Fisher Scientific, Waltham, MA, USA). Muscle sections were dried for 2 hours at -20°C ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were buffer exchanged against 250 mM ammonium acetate (pH 7.0) using Zeba spin desalting columns with a 7 kDa cutoff (Thermo Fisher Scientific, Waltham, MA, USA). The PulLCTD-PulMCTD complex was either formed prior to or after buffer exchange by mixing PulMCTD with an equimolar or a 2-fold molar excess PulLCTD.
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bioRxiv - Pathology 2023Quote: Total RNA extracted from carotid artery lysates was reverse transcribed and amplified in a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA). Primers were designed by Integrated DNA Technologies to amplify the following genes ...
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bioRxiv - Immunology 2024Quote: ... The cDNA was used as template for the qPCR reactions using Bio-Rad’s SYBR® Green supermix using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA). The primers used to quantify mRNA are listed in Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with aptamers at reported concentrations or with vehicle (DPBS and Mg2+) for 48 h and then stained according to the CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific, cat: #C10427). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 2.5 µM DCFH-DA (2’,7’-dichloro-dihydro-fluorescein-diacetate; excitation at 495 nm, emission at 520 nm; Life Technologies, Europe BV, Stockholm, Sweden) in Hank’s buffered saline solution without phenol red for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Gene expression levels were evaluated using quantitative real-time PCR analysis (qPCR) and Quant Studio 7 Flex System (Applied Biosystems, Foster City, CA, USA). The following TaqMAN probes were used ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... except for Fig 6 and Fig 7 which the recovered cells were counted by inclusion of counting beads (Accu-Check, Molecular Probes, Thermo Fisher Scientific) in the FACS sample according to manufacturer’s instructions and calculated using the equation ...
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bioRxiv - Neuroscience 2023Quote: ... saline (N = 7) or muscimol (N = 8, conjugate of muscimol and the Bodipy TMR-X fluorophore, InvitrogenTM; purchased from Thermo Fisher Scientific, reference M23400) disolved in PBS (ph 7.4 ...
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bioRxiv - Plant Biology 2024Quote: The production of ROS in guard cells of CrSAL1 transgenic and WT plants was measured using a fluorescent indicator 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA, Life Technologies, Waltham, MA USA) (Cai et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight incubation in a cold room (6-7°C) was carried and anti-mouse/rabbit secondary antibody Alexa-488 conjugated (Thermo Fisher Scientific, Waltham, MA) incubation was performed (1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... for 7 days followed by the following respective secondary antibodies: donkey anti-rabbit conjugated to Alexa-488 (1:1000, Thermo Fisher Scientific, # A-21206) and donkey anti-chicken conjugated to Alexa-647 (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... mononucleated resident cells were isolated from human muscle as described in “Fluorescence-activated MuSC sorting and culturing” part with 7-AAD (Thermo Scientific, 00-6993-50) staining for viability selection ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... All assays were run using 384-well optical plates on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™, Thermo Fisher). RNA-Seq libraries were prepared according to the Illumina RNA-Seq protocol and sequenced at the Genomics Unit of Instituto Gulbenkian de Ciência using a NextSeq system to generate single-end 75-bp reads.
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Plant Biology 2024Quote: ... extracted with 3 mL methanol for 1 h shaking at 200 rpm at 37 °C in a 7 mL glass scintillation vial (Fisher Scientific 03-337-26). The methanol extracts were dried under nitrogen gas and resuspended in 3 mL of deionized water ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... All assays were run using 384-well optical plates on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™, Thermo Fisher). RNA-Seq libraries were prepared according to the Illumina RNA-Seq protocol and sequenced at the Genomics Unit of Instituto Gulbenkian de Ciência using a NextSeq system to generate single-end 75-bp reads.
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bioRxiv - Immunology 2021Quote: Tendon samples were homogenized for 45 seconds at 4 m/s in 600 μL TRIzol reagent using a Fisherbrand™ Bead Mill 4 Homogenizer (Thermo Fisher Scientific, 15-340-164) with reinforced 2 mL tubes (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... the cells were stained with CD3 BUV805 or CD3 AF700 (4:100 or 4:100; BD Biosciences Cat# 612895 or Life Technologies Cat# 56-0038-42, respectively), CD4 BV605 (4:100 ...
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bioRxiv - Plant Biology 2020Quote: ... and quantified using a Qubit 4 Fluorometer (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Biophysics 2021Quote: ... Nucleus were stained with DAPI (4’, 6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... while others were perfused and fixed in 4% paraformaldehyde (Fisher Scientific) overnight at 4°C ...