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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... made of 5:1:1 ratio of H2O : H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... Antibodies were NKX2-5 (mouse, R&D, 1:500) and β -actin (rabbit, Invitrogen, 1:1000). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... the media was changed to 1:1 KSR: N2B media with puromycin (5 μg/ml, Gibco), where N2B was composed of DMEM/F12 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary GR antibody (G-5, Santacruz sc-393232, 1:100) or MAP2 antibody (Invitrogen, 1:2000) incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Cell Biology 2023Quote: ... centrifuged at 1,000 rpm for 5 min and resuspended in DMEM:F-12 (1:1) (Gibco, C11330500BT) media supplemented with 10% FBS and 1% penicillin- streptomycin ...
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bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific, Scornsby, VIC, AUS), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were blocked for 1 hour in 2 mg ml−1 BSA and 1% Goat Serum (Thermo Fisher) in 1X PBSTXD (1X PBS ...
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bioRxiv - Immunology 2024Quote: ... CD206-FITC (19.2, 1:100), CD11c-PE (3.9, 1:50) and CD14-PerCP-Cy5.5 (61D3, 1:200) from eBiosciences (ThermoFisher) were used.
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with goat anti-rabbit Alexa 647 (1:500 or 1:1000, 2 hours, ThermoFisher) and in the case of biocytin filling ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with 2° antibody (1:1500) and Alexa FluorTM 647 Phalloidin (Invitrogen A22287, 1:2000) in PBTB for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 106 cells were injected subcutaneously (resuspended in 1:1 media:Geltrex™ (Thermo Fisher A1413201)).
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bioRxiv - Microbiology 2023Quote: 1.5-2 μg total RNA was diluted 1:1 in 2x sample buffer (Invitrogen™), loaded onto 7.5% TBE-Urea PAGE gels ...
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bioRxiv - Cell Biology 2023Quote: ... For control knockdown a 1:1 mixture of Silencer Select Negative Control #2 (Ambion, 4390846) and Silencer Select Negative Control #1 (4390843 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048), 1 % L-Glutamine (Biological Industries #03-020-1B) ...
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bioRxiv - Molecular Biology 2021Quote: ... YME1L1 knockout-HEK293 cells were grown in Dulbecco’s modified Eagle’s medium (with 1 mM pyruvate) supplemented with non-essential amino acids (Gibco).
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bioRxiv - Microbiology 2019Quote: ... 1 mM ethylene-diamine-tetra-acetic acid buffer (pH 8.0) and stained with SYBR Gold (Life Technologies, Singapore). Samples were incubated in the dark for 15 minutes prior to analysis ...
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bioRxiv - Molecular Biology 2021Quote: African green monkey kidney epithelial VeroE6 (ATCC) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermofisher, supplemented with 1% Non-Essential Amino-Acids (Thermofisher), 10mM HEPES (Thermofisher ...
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bioRxiv - Biophysics 2020Quote: ... containing 1% non-essential amino acids and 10% heat inactivated Fetal Bovine Serum (FBS, Gibco, Thermo Fisher Scientific) under standard cell culturing conditions (at 37 °C and 5% CO2) ...
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bioRxiv - Biophysics 2020Quote: ... containing 1% non-essential amino acids and 10% heat inactivated Fetal Bovine Serum (FBS, Gibco, Thermo Fisher Scientific) under standard cell culturing conditions (at 37 °C and 5% CO2) ...
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bioRxiv - Pathology 2020Quote: ... Total ribonucleic acid of 1 μg was used for the first-strand complementary DNA synthesis (Thermo Fisher Scientific). Quantitative real-time polymerase chain reaction was performed by using Quant Studio 3 (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were suspended in FacsMax (amsbio) and stained with SytoxTM green nucleic acid stain (1:100000, Invitrogen) and Hoechst (1:5000 ...
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bioRxiv - Systems Biology 2020Quote: ... Combined eluates were acidified with trifluoroacetic acid at final concentration of 1% (TFA, mass spec grade, Thermo Fisher) and desalted with a C18 stage tip.
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bioRxiv - Microbiology 2019Quote: ... 1.2% SDS, 12 mM EDTA) and 700 µl hot acid-phenol:chloroform pH 4.5 (with IAA, 125:24:1) (Ambion). After incubating at 65°C for 30 min with shaking at 1500 rpm ...
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bioRxiv - Physiology 2019Quote: ... The pH of the 1% acetic acid solution measured 2.6 using an Accumet AE150 pH probe (Fisher Scientific). The estimated pH once applied to the airway surface is ∼6.6 to 6.8 (22) ...
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bioRxiv - Microbiology 2020Quote: ... Two additional experimental conditions were added: 1) uninfected skin with 10 µl 100% oleic acid (Thermo-Fisher Scientific) applied to the surface of the skin explant and 2 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were transferred to bacteria-seeded plates containing 1 mM indole-3-acetic acid (Acros Organics, Cat #122160250) and incubated for the indicated time periods before analysis.
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of GlycoBlue (to improve pellet visualisation) and 400 μL of Acid-Phenol:Chloroform (pH 4.5; Thermo Fisher) were added ...
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bioRxiv - Biochemistry 2024Quote: ... the gel was stained with 1× SYBR Gold Nucleic Acid Gel Stain for 30 min (Thermo Fisher Scientific). The gel image was quantified by ImageJ (33) ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured in the MEM medium supplemented with 10% FBS and 1% nonessential amino acids (Gibco).
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bioRxiv - Biochemistry 2023Quote: ... Samples were then vacuum dried and resuspended in 50 μL of 1% formic acid (Thermo Fisher Scientific, 695076). Sample peptides were calculated via CBQCA quantification kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM ethylenediaminetetraacetic acid] buffer supplemented with 1x HALT protease and phosphatase inhibitor cocktail (Pierce Thermo Fisher Scientific) for 15 min at 4 ℃ ...
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bioRxiv - Biochemistry 2024Quote: ... and FITC conjugated lectins (SNA, recognizing alpha 2,6 linked sialic acids, and UEA-1, recognizing alpha 1,2 linked fucose, Invitrogen) were used to stain glycosylated mucins ...
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bioRxiv - Plant Biology 2024Quote: ... mVenus was cloned solely or with an open reading frame of SUF4 isoform 1 (NP_564369.1; 367 amino acid) in the modified pFastBac vector (ThermoFisher). Site-directed mutagenesis was carried out to create the 5S and 12S mutants using PrimeSTAR Max DNA polymerase (Takara-bio) ...
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bioRxiv - Bioengineering 2021Quote: ... or a long-chain fatty acid (Hexadecanoic Acid, C16; BODIPY™ FL C16, D3821, Thermo Fisher Scientific Inc.) were added at a concentration of 4 µM to the culture medium as defined by culture mode (table 3) ...
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bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from tissues using the MELT™ Total Nucleic Acid Isolation System (Ambion, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... omitting all amino acids without the addition of non-essential amino acids and substitution with dialyzed FBS (Invitrogen). Doxycycline treatment of cells stably expressing ER-phagy probe was performed as previously described7.
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bioRxiv - Pathology 2023Quote: ... Dilutions of SCFAs standards (Acetic acid:A6283, Sigma-Aldrich; Propionic acid: 81,910, Sigma-Aldrich; Butyric acid:108,111,000, Acros Organics) were prepared in saline solution at concentrations ranging from 87 to 0.087 mM for acetic acid ...