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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
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bioRxiv - Cell Biology 2020Quote: Cells from one-day-old culture - at the mid-exponential growth phase - at an initial cell density of 1×105 cells / ml were seeded in 12-well plates (Nunc/DD Biolab, #55428) with 1 ml marine broth and incubated at 23 ºC ...
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bioRxiv - Plant Biology 2020Quote: Four to six-days old seedlings were incubated in 24-well cell-culture plates for 1 hour in 50 μM BFA (Invitrogen, Thermo Fisher Scientific) containing liquid growth medium (0.5x MS medium ...
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bioRxiv - Immunology 2020Quote: ... monocytes were allowed to adhere to culture plates (Costar) for 1 h at 37°C in warm RPMI-1640 medium (ThermoFisher Scientific, Waltham, MA). The cells were then washed with warm phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... according to manufacturer’s protocol (primers listed in Table 1) - each sample was run in triplicate reactions in 96-well reaction plates (MicroAmp® Fast Optical, Thermo Fisher Scientific) in a 96-well Real-Time PCR instrument (StepOnePlusTM Real-Time PCR System ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in an undifferentiated state under feeder-free conditions on GelTrex-coated plates (diluted 1:1000 from 15 mg/ml) (Thermo Fisher Scientific, USA). Cells were fed daily with mTeSR medium (STEM CELL Technologies ...
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bioRxiv - Microbiology 2020Quote: Serum samples were heat-treated at 56°C for one hour9 and diluted 158-fold in 1% skim milk in PBST and placed in a 96-well polypropylene plate (ThermoFisher, cat # AB-0796), which served as a master plate ...
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bioRxiv - Immunology 2020Quote: ... remaining cells were seeded into a 12-well plate with 1 × 106 cells per well in 2 ml culture medium consisting of DMEM-GlutaMAXTM (Gibco, ref.no: 31966-021), supplemented with penicillin (50 I.U./mL) ...
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bioRxiv - Immunology 2019Quote: ... monocytes were allowed to adhere to 24-well plates at 5×105 cells/well for 1 h at 37°C in warm RPMI-1640 medium (ThermoFisher Scientific, Waltham, MA). The medium was then supplemented to a final concentration of 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... at 37° C 5% CO2 in 96 well plates in the presence of IL2 (GIBCO 100 IU/mL or Roche 1 ng/mL). Additional conditions included 1 ng/mL TGFβ (GIBCO) ...
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bioRxiv - Neuroscience 2021Quote: ... and adjusted tissue and plasma extracts were added with the antiserum against OT (1:1,000 dilution) to each antigen-coated well of a 96-well microplate (F96 maxisorp nunc-immuno plate;Thermo Fisher Scientific, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells cultured overnight (1×105 cells/well in 6-well plates) were transfected with plasmid and 5μl Lipofectamine 2000 (Thermo Fisher Scientific, United States). The cells were then incubated at 37 °C for 24 h before analysis.
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bioRxiv - Biochemistry 2024Quote: ... Dried extract was reconstituted in 1 mL of LC-MS-grade water and desalted by Sola HRP SPE 30mg/2MI 96-well plate 1EA (Thermo Scientific #60509-001). Desalting cartridges were cleaned three times with 1 mL of methanol ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were seeded in 2 wells of a vitronectin-coated 24-well plate with StemMACS™ PSC-Brew XF supplemented with 1:200 RevitaCell (Thermo Fisher, A2644501). Cells were kept for 2-3 days ...
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bioRxiv - Pathology 2023Quote: ... Primers in a final concentration of 500 nM each and DNA volume of 1 µl (<50ng) were added to the PCR master mix in MicroAmp Fast 96-Well reaction plates (4346907, Thermo Fisher Scientific, USA). qPCRs were performed using QuantStudio 6 system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... MSD GOLD 96-well small-spot streptavidin-coated microtiter plates (Meso Scale Discovery, Rockville, MD) were treated with a blocking step of 1% casein-based PBS (Thermo Scientific, Waltham, MA) for approximately 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were re-activated in a non-culture treated 24 well plate that was coated with aCD3 and aCD28 (1:200 in PBS, Invitrogen, 16-0037-85) and aCD28 (1:200 in PBS ...
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bioRxiv - Cell Biology 2023Quote: 20^106 HCT116 WT and KO1 cells were crosslinked by adding on the plate culture media with 1% formaldehyde (Thermo Fisher Scientific; #28908) for 10 min at room temperature shaking ...
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bioRxiv - Immunology 2023Quote: BMDMs were prepared and seeded into 12-well cell culture plate or 96-well cell culture plate at a density of 1 x 106 cells/mL of RPMI-1640 medium (Thermo scientific, Cat# 61870036) containing 10% of Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... For each transformation 63 µL of protoplast suspension was mixed with 25 µL of DNA in 0.1 M Ca(NO3)2 and 88 µL of 40% (w/w) PEG solution in a round-bottom 1 mL 96 deep-well plate (Nunc®, Roskilde, Denmark). The PEG solution was prepared by mixing 8 g of PEG (polyethylene glycol ...
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bioRxiv - Biophysics 2023Quote: FACS-purified MΦTR were seeded onto 15-mm glass coverslips in 24-well plates at a density of 20,000 cells/mL in 1 mL Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal calf serum and 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2023Quote: ... a dilution series of either DnaJA2 or JDMs was prepared and added to Hsc70 (1 µM) in 384-well plates using a Multidrop dispenser (Thermo Fisher Scientific, Inc.). Quinaldine red solution was freshly prepared each day as a 2:1:1:2 ratio of 0.05% w/v quinaldine red ...
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bioRxiv - Neuroscience 2024Quote: ... UK) and 1:5 diluted dye reagent pipetted in separate wells in a standard 96 well microtiter plate (Thermo Fisher Scientific, Waltham, USA). The plate was immediately mixed and measured at 595 nm using the Milenia Kinetic Analyzer (Diagnostic Product Cooperation ...
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bioRxiv - Immunology 2024Quote: ... monocytes were allowed to adhere to 24-well plates at 5×105 cells/well for 1 h at 37°C in warm RPMI-1640 medium (ThermoFisher Scientific, Waltham, MA). The mean purity of adherent monocytes was 85 % (range ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplicons of these four primer pairs were then purified from 1% agarose gel using Qiagen Gel Extraction kit and concentrations were measured with Qbit High Sensitivity DNA kit (Thermo Fisher Scientific). Each amplicon was then diluted to 1×109 molecules per µl ...
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bioRxiv - Genetics 2020Quote: ... Barcoded products were pooled and gel purified from a 1% agarose gel using a QIAgen kit (#28115) to remove primers before quantitation with a Qubit dsDNA HS Kit (ThermoFisher Scientific #Q32851). Samples were loaded onto an Illumina MiSeq instrument with a 300 cycle v2 kit for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the QIAquick Gel Extraction Kit after electrophoresis in agarose gel 1% and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2024Quote: ... and the sequencing library was prepared using the Ion XpressTM Plus Fragment Library Kit and the Ion XpressTM Barcode Adapters 1-96 kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and 20 μl of such mixtures were loaded on 96-well white plates (Microamp Optical 96-well Reaction Plate with Barcode, Applied Biosystems, #4306737), containing 5 μl of an appropriate 5x buffer ...
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bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
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bioRxiv - Molecular Biology 2019Quote: ... The experiments were carried out using the CLARIOstar plate reader (BMG Labtech; settings: orbital reading 4 mm (for 96-well plates from Nalge Nunc International) or 2 mm (for 96-well plates from Thermo Scientific) ...
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bioRxiv - Immunology 2019Quote: ... 1×106 (24-well plate) or 5×105 (48-well plate) CD8+ T-cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher, 11456D) in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP ...
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bioRxiv - Microbiology 2019Quote: ... The solution was transferred into a fresh plate and absorbance measured at 590 nm with a plate reader (Multiskan Go, Thermo Scientific, USA). Results were exported with the software SkanItTM (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... Microtiter plate cultivations were performed in sterile 96-well plates (flat bottom Nunclon™ Delta Surface, Thermo Fisher Scientific, Waltham, MA, US) containing 200 μL LB per well ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed three times in DPBS and 100 μl of DPBS was added to each well and plates were sealed with adhesive plate tape (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2021Quote: ... the content of the wells was homogenised by pipetting up and down and 200 μL of suspension were transferred into a microplate-reader compatible plate (Fisherbrand 96-Well plates, Fisher Scientific, USA). The OD was measured at 600 nm (Infinite M200 PRO ...
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bioRxiv - Immunology 2021Quote: ... 1×106 (24-well plate) or 5×105 (48-well plate) CD8+ T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher, 11456D) in a 1:1 bead to T cell ratio and cultured in 2 ml (24-well plate ...
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bioRxiv - Immunology 2022Quote: ... The unlabeled tumor cells and transduced T cells were added to a 96 Well Black Plate glass bottom plate (Thermo Scientific, 160376). 60-70 z-stacks (0.2μm steps ...
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bioRxiv - Systems Biology 2022Quote: ... cells were seeded at 8000 cells/well (96 well plate) or 35000 cells/well (24 well plate) in media without phenol red (Thermo Fisher 11054020) with glutamine supplemented (Life Technologies 35050-061) ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Biochemistry 2024Quote: ... The Multiplate 96-Well PCR Plate is then incubated at 37 °C in a 96-well Non-Skirted PCR Plate Block (Thermo Scientific #88870120) in a digital dry bath (Thermo Scientific #88870006).
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: dLN single-cell suspensions from C57BL/6 infected mice were cultured in 96 well plates “U” bottom with 5μg/mL of plate-bound anti-CD3 (clone 145-2C11, Invitrogen, Waltham, MA, USA), 0.5 μg/mL soluble anti-CD28 (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The assays were conducted in sterile Nunclon® 96 microwell plates and Nunc® 384 well polystyrene plates purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with 2ug per well (6 well plate) and 1ug per well (12 well plate) of plasmids concentration with the transfection reagent Lipofectamine 3000 (Invitrogen™, L3000001) and the protocol mentioned in the kit was followed ...
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bioRxiv - Systems Biology 2023Quote: ... The 96-well plate was centrifuged to pellet cells and the pellets were pinned to a YPD + G418 + NAT plate (Nunc Omnitray #165218) and grown 2 days at 30°C to select for mated MATa/α diploids ...
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bioRxiv - Synthetic Biology 2023Quote: ... The phage kinetics were performed in flat bottom transparent 96 bacterial culture well-plates with lids (Thermo Scientific Optical-Bottom Plates, #265301) on a Synergy H1 multi-mode microplate reader (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... pre-cultures were prepared by inoculating single colonies from a LB agar plate in 200 μl of liquid LB in a 96-well plate (Nunc, Thermo Scientific). After 22 h of growth at 37°C with 250 rpm shaking ...