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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the protein amount of the supernatant was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and Synergy LX Multimode Reader (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... and then sonicated before protein concentration was calculated using the EZQ protein quantification kit (Thermo Fisher Scientific, R33200). Each of the 3 Mouse biological replicates were separated into two aliquots each containing 200 μg of protein ...
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bioRxiv - Cell Biology 2023Quote: ... Protein loading was equilibrated by measuring concentration on a Qubit using the Protein BR kit (Thermo Fisher Q33211). 20-30μg lysate was boiled for 10 minutes with loading dye (Thermo Fisher 26634 ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were measured with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). The collected supernatant (180 μL ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in each faction was determined by a BCA protein assay kit (Thermo Fisher Scientific, PI23227). Immunoblots were performed as described previously (Farooq et al. ...
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bioRxiv - Neuroscience 2023Quote: ... OCRs were normalized to the protein concentrations determined by the Pierce BCA Protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was measured using the Pierce® BCA Protein Assay Kit following the manufacturer’s instructions (Thermo Scientific). The samples were resolved by electrophoresis on ExpressPlus PAGE precast gels 4–20% (GeneScript) ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of protein samples were measured with the Micro BCA™ Protein Assay Kit (Life Technologies Cat#23235), using a BSA standard curve ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration of each sample was determined using the PierceTM BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Purified proteins were labeled with Alexa Fluor 488 fluorescent dye using Microscale Protein Labeling Kits from Thermo Fisher, following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Cat: A53225; Thermo Fisher Scientific) and western blotting.
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bioRxiv - Cell Biology 2023Quote: Newly synthetized proteins were labelled using the Click-iT HPG Alexa Fluor488 Protein Synthesis Assay Kit (Thermo Fisher). Neurons were incubated with 0.1 mM L- homopropargylglycine (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Total protein concentrations were measured in cell lysates using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). A total of 15 µg of total protein fraction for each sample was separated by electrophoresis using NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... The total protein yield was determined by Pierce Bradford assay (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific). Proteins were then transferred to membranes and immunoblotting was performed using the antibodies described in Supplementary Table 2.
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then quantified using the Pierce™ BCA Protein Assay Kit (Cat # 23227; Thermo Fisher Scientific) in a 96-well plate format (Cat # 353072 ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentration of the supernatants was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher) and adjusted to equal input levels ...
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bioRxiv - Physiology 2023Quote: ... and the protein concentration was determined using the Pierce BCA Protein Assays Kit (germo Fisher Scientific, Rockford, IL) Protein samples (25 μg ...
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bioRxiv - Neuroscience 2023Quote: ... the protein concentration of each sample was measured using the Micro BCA™ Protein Assay Kit (Thermo Fisher), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the protein concentration was determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of the samples were determined using a Thermo BCA protein assay kit (Thermo Fisher Scientific) and adjusted to 1 mg/ml for SDS‒PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... The concentration of protein was determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher, A53225). Aconitase was measured according to the Aconitase Activity Assay Kit.
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bioRxiv - Cell Biology 2024Quote: ... protein concentration was determined using PierceTM BCA Protein Assay Kit (Cat. No. 23225, Thermo Scientific, Waltham, MA, USA) and equal protein amounts were mixed with SDS-containing Laemmli buffer and loaded onto SDS-polyacrylamide gels ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was quantified on a plate reader using the Pierce BCA protein assay kit (#23225, Thermo Scientific). Gel lanes were loaded with 30 µg of total protein ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in each biological replicate was measured by Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit ...
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bioRxiv - Neuroscience 2024Quote: Protein synthesis was assessed using the Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations in cell lysates were then normalized with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration was assayed in the supernatants using the Pierce BCA Protein Assay Kit (Fisher Scientific, 23225). GCase activity measurements were performed as previously described17 ...
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bioRxiv - Bioengineering 2024Quote: ... The protein concentration of each sample was determined by BCA assay (Thermo Scientific, Pierce BCA Protein Assay Kit) relative to a bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... The protein concentration was determined in the samples using the Pierce BCA Protein Kit (Thermo Fisher Scientific Inc). The solid protein was denatured at 95°C for 5 min in NuPAGE LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of lysates was tested by the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). For the reducing gel ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein fractions were quantified using an 8-point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Molecular Biology 2024Quote: ... protein concentrations of sheared chromatin were quantified with the Pierce™ BCA Protein Assay Kit (Thermo Scientific, #23227) following manufacturer instructions.
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bioRxiv - Bioengineering 2024Quote: ... Total protein concentration was quantified by Micro BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific). Samples and migration standards were prepared under reducing conditions and incubated for 10 min at 37 °C prior to loading ...
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bioRxiv - Physiology 2024Quote: ... The protein concentration in the supernatant was then determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). For protein analysis in isolated mitochondria ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was kept it in ice and protein concentration were measured with QUBIT protein kit (#Q33211, ThermoFisher).
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bioRxiv - Developmental Biology 2024Quote: ... The amount of protein in the lysate was quantified by NanoOrange™ Protein Quantitation Kit (Thermo Fisher Scientific) and the enzymatic activity of glutamine synthetase was measured by Glutamine Synthetase Activity Assay Kit (Abcam) ...
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bioRxiv - Developmental Biology 2024Quote: ... The lysate protein content was quantified with the Pierce™ BCA Protein Assay Kits (ThermoFisher Scientific, Cat.#: 23225) following the manufacturer’s recommendations ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The EV protein concentration was measured by using a Micro BCA Protein Assay Kit (23235, Thermo Fisher Scientific). The collection method ...
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bioRxiv - Cancer Biology 2024Quote: ... and estimated the protein concentrations using Pierce BCA Protein Assay Kit as per the manufacturer’s protocol (ThermoFisher Scientific). Around 10 µg of proteins were allowed to resolve in 8% SDS-PAGE separating gel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). The concentration of samples was adjusted by dilution with RIPA buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
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bioRxiv - Neuroscience 2020Quote: ... was added to the plate after which a colour reaction was generated by incubation with TMB (3,3’,5,5’-tetramethylbenzidine) colorimetric substrate (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher Scientific). The reaction was stopped with 2 M H2SO4 and the plate read at 405nm with a Tecen Infinite M1000 plate-reader ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plates were washed three times and then developed using 50 μL 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher).
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bioRxiv - Biochemistry 2022Quote: ... followed by three washes in 1% BSA and incubation with TMB-ELISA substrate (Thermo Fisher Scientific, Cat. no. 34028) until the light blue color appeared ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 15 min at room temperature with 1-Step Turbo TMB-ELISA Substrate Solution (Thermo Fisher), then terminated with equal volume of 1M phosphoric acid ...
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bioRxiv - Biochemistry 2021Quote: ... Binding was visualized through the addition of 50 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher, #34028), incubated in the above plate shaker for approximately 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... ZIKV-NS1 and MV-H were similarly resuspended in coating buffer (50 mM Na2CO3 [pH 9.6]) at a concentration of 1 μg/ml and then plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl per well ...
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bioRxiv - Microbiology 2020Quote: ... was resuspended in a coating buffer (50 mM Na2CO3 [pH 9.6]) at a concentration of 1 μg /ml and then plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl in each well ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plates were washed three times and then developed using 50 μL 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher). A similar ELISA protocol was followed for detecting RNC complexes displaying glycosylated DQNATpd except that the plates were coated with 50 μL of 5-μg/mL mouse mAb FB11 in PBS and were subsequently detected with HRP-conjugated anti-mouse IgG.