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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: Nonsense control (NC) or human PKD2 siRNA were transfected by Lipofectamine 2000 (11668-027; Invitrogen) in HK2 cells using DMEM/F12 medium containing 10% fetal bovine serum according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
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bioRxiv - Genomics 2024Quote: Human iPSCs were cultured in Essential 8 Medium (Gibco/Thermo Fisher Scientific; Cat. No. A1517001) on 6-well plates coated with Matrigel Matrix (Corning ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: Human SH-SY5Y neuroblastoma cells obtained from ATCC (CRL-2266) were grown in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: Human HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Cat# 084564, Gibco, US) supplemented with fetal bovine serum (10% ...
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bioRxiv - Immunology 2024Quote: Human Tissue: Cells were first incubated with Fixable Blue Live/Dead Dye (Thermo Fisher Scientific) and TruStain FcX (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... Human retinal pigment epithelial (ARPE) cells (ATCC CRL-2302) were cultured in DMEM-F12 (Gibco), with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Primary human muscle stem/progenitor cells were plated in F10 basal medium (Gibco, cat#11550043) containing 20% FBS (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... alamarBlueTM Cell Viability Reagent and human dermal fibroblast (HDF) cells (C0135C) were obtained from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Human breast cancer cells (SKBR3; ATCC, HTB-30) were cultured in McCoy’s 5a medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... + 500 U/mL Gibco™ recombinant human (rh) IL-2 (Thermo Fisher Scientific, cat# PHC0021). For primary NK cells ...
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bioRxiv - Cancer Biology 2024Quote: Human AML cell lines (Supplemental Table 1F) were maintained in minimum essential medium-alpha (Gibco) supplemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Immunology 2024Quote: Murine TNF and IL-1β were quantified in cell supernatants using the ELISA™ Kit from Invitrogen and human IL-1β was similarly quantified with the Instant ELISA™ Kit from Invitrogen, following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... human HEK 293T cells were cultured at 37 °C in 5% CO2 in DMEM (ThermoFisher), with 10% FCS and 1% P/S ...
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bioRxiv - Cancer Biology 2024Quote: ... RWPE human prostate cancer cell line was grown in Keratinocyte serum free medium (Thermo Fisher) supplemented with human recombinant epidermal growth factor (rEGF ...
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bioRxiv - Molecular Biology 2024Quote: ... and U2OS (ATCC 30-2007) human osteosarcoma cells were maintained in McCoy’s 5A media (Gibco). Media for HEK293 ...
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bioRxiv - Cell Biology 2024Quote: ... The human islets were picked under stereomicroscope by hand and cultured in CMRL Medium (Gibco) with 5.5 mM glucose supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... and 100U/ml PeproGMP™ Human IL-2 Recombinant Protein (Thermo Fisher; 200-02-1MG). The T cell culture was transferred to G-Rex bioreactor vessels (Wilson Wolf ...
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bioRxiv - Immunology 2024Quote: ... Freshly isolated human PMN were resuspended in Hank’s Balanced Salt Solution (HBSS, Gibco™ #24020117) supplemented with 25 mM glucose (mHBSS ...
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bioRxiv - Biochemistry 2024Quote: Media for adherent human-derived U251 glioma cells was prepared using 500 mL DMEM (Gibco) with the following additives ...
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bioRxiv - Cell Biology 2024Quote: Differentiated human myotubes were serum-starved for 4 hours in low-glucose (Gibco, 5.5 mM) and then were incubated for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: Differentiated human myotubes were serum-starved for 2 hours in low-glucose DMEM (Gibco, 5.5mM) and then were incubated for 6 hours in low-glucose DMEM supplemented with 25μM of palmitate ...
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bioRxiv - Microbiology 2024Quote: ... This medium consisted of RPMI-1640 medium containing 10% human serum and 10% AlbuMAXI (Invitrogen), along with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were activated using human CD3/28 Dynabeads (cat. 11131D, Thermo Fisher Scientific) in RPMI medium ...
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bioRxiv - Microbiology 2024Quote: Human lung adenocarcinoma cells (ATCC CCL-185) were maintained in Dulbecco’s minimal essential media (Gibco) supplemented with 10% fetal bovine serum (Seradigm ...
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bioRxiv - Microbiology 2024Quote: ... BA.2.86 isolated) were human codon-optimized and produced in silico (GeneArt, Thermo Fisher Scientific) as described 42,44 ...
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bioRxiv - Molecular Biology 2024Quote: ... the media was harvested and vWF was detected using the vWF Human ELISA Kit (Invitrogen) according to manufacturer specifications.
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bioRxiv - Immunology 2024Quote: Each human peripheral blood leukopak was washed with 80 ml serum free RPMI 1640 (Gibco), and was overlaid on lymphoprep (STEMSELL ...
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bioRxiv - Cell Biology 2024Quote: Differentiated human myotubes were serum-starved for 2 hours in low-glucose DMEM (Gibco, 5.5mM) Then ...
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bioRxiv - Genetics 2019Quote: ... cells were subjected to lysis and firefly luciferase and β-galactosidase enzymatic activities were measured consecutively using a dual-luciferase reporter assay system (Applied Biosystems, Bedford, MA) as per the manufacturer’s instructions ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... using crude extracts from plant leaves and monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4-methylumbelliferyl-β-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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bioRxiv - Neuroscience 2019Quote: ... axons of the visual pathway were labeled by intravitreal injection of 2 µl of Alexa Fluor 555-conjugated cholera toxin β subunit (0.5% CTB, in PBS; Molecular Probes, Carlsbad, Eugene, USA) and tissues isolated after another week.
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bioRxiv - Physiology 2020Quote: Gene expression was measured using Applied Biosystems Taqman gene expression assay probes for Hamp and house-keeping gene β-Actin (Life Technologies, Carlsbad, CA). The CT value for the gene of interest was first normalised by deducting CT value for β-Actin to obtain a delta CT value ...
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bioRxiv - Cell Biology 2019Quote: ... pCMV/myc/nuc/GFP which consists of GFP fused to 3x SV40 nuclear localization signals whose nuclear import is mediated by importin α/β (Invitrogen V821-20), pmCherry-C1-RanQ69L (Addgene #30309 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with a length of roughly 900 bp (the consensus length of the conserved Na,K-ATPase β-subunit) using the program VectorNTI (Thermo Fisher, Darmstadt, Germany). Homology of the contigs were assigned by a further round of tblastx searches against published sequences in genbank.
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bioRxiv - Cell Biology 2020Quote: ... n-dodecyl-β-D-maltoside and assessed for OxPhos complex content using a Native PAGE Novex Bis-Tris Gel system (Invitrogen, Carlsbad, CA, USA). The separated proteins were transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Purification of α and β cells by FACS) or from pelleted whole islets using the Invitrogen RNAqueous-Micro Total RNA Isolation kit (Thermo Fisher #AM1931). TURBO DNA-free (Ambion ...
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bioRxiv - Developmental Biology 2020Quote: ... in water) in complete medium containing 1000 U/mL leukemia inhibitory factor (LIF; MilliporeSigma, ESG1106) and 100 μM β-mercaptoethanol (BME; Thermo Fisher Scientific, 21985023) at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Flow SA-β-Gal staining assay was performed by flow cytometry using an ImaGene Green C12FDG lacZ gene expression kit from Molecular Probes (Life Technologies), according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... The gene expression level relative to β-actin was determined by qPCR with gene-specific primers (Supplementary Table S1) and PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, USA). 2-ΔΔCT was calculated for relative fold change.
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bioRxiv - Microbiology 2023Quote: ... β-galactosidase activity was measured using the Tropix Galacto-Light Plus™ chemiluminescent kit according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA).
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bioRxiv - Microbiology 2022Quote: ... β-galactosidase activity was measured using the Tropix Galacto-Light Plus™ chemiluminescent kit according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA).
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bioRxiv - Microbiology 2023Quote: ... mutans was visualized via Alexa Fluor 555 (Life Technologies; B40955. For another antibody pair, fungal β-D glucan was visualized via Alexa Fluor 647 (Life Technologies; B40958) and Gram positive bacteria LTA was visualized via Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 1.43M β-mercaptoethanol was added to the samples to reach a 1X dilution (350 mM β-mercaptoethanol final concentration) before gel electrophoresis performed using 4–12% Bis-Tris gels (ThermoFisher Scientific, prod. number WG1402BOX). Proteins were transferred on Immobilon-FL membrane (Millipore ...
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bioRxiv - Pathology 2021Quote: ... SDS-PAGE gel was followed by immunoblotting using primary antibodies against pAkt, Akt, pAkt1(Thr 308), Akt1, HIF-1α, HIF-2α, β-Actin (Cell Signalling, USA) and α-tubulin (Thermo Fisher Scientific, USA) as described in detail in our previous work (45) ...
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bioRxiv - Genomics 2022Quote: ... RNA extractions were performed following the previously described pine tree extraction method [38] used in combination with β-mercaptoethanol [39] and TRIzol (Life Technologies, Corp., Carlsbad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... Equal loading of protein samples from each group was evaluated using β-actin or GAPDH antibody after using Restore WB stripping buffer (Thermo Fisher Scientific, catalog 21059).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was co-transfected with 5 μg tk-(MH100)4-luciferase reporter and 5 μg CMX-β-galactosidase transfection control plasmids in Opti-MEM using Lipofectamine 2000 reagent (Invitrogen Life Technologies, Grand Island, NY), following the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana genome was fused upstream of the GUS (β-glucuronidase) reporter gene in pENTR vector (pENTR/D-TOPO Cloning Kit, Thermo Fisher Scientific, Waltham, USA) and transferred into the binary vector pGWB1 (Nakagawa et al. ...
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bioRxiv - Physiology 2023Quote: ... Excess dye was removed via cold acetone precipitation (−20°C for 20 min) and samples were resuspended in 50μL of 1X reducing buffer (Invitrogen loading dye and 5% β-mercaptoethanol), heated for 5 mins (95°C ...