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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Neuroscience 2024Quote: ... Compound (ref # 4583, Sakura Finetek) by submerging it in 2-Methylbutane (ref # O3551-4, Fisher Scientific, Pittsburgh, PA) cooled with dry ice to −80°C ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants (2 mg protein) were removed and incubated overnight at 4°C with Dynabeads Protein G (Thermo Scientific) coated with anti-FLAG antibody or IgG heavy chain according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced by the B-27 medium (Neurobasal medium supplemented with 2% B-27 serum free (Gibco), 1% GlutaMax and 1% EmbryoMax 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... N-2 media was replaced by B-27 media consisting of B-27 Serum-Free Supplement (Thermo Fisher Scientific), GlutaMAX (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... A 9:1 ratio of DNase I buffer (10X) (Invitrogen) to RNA sample respectively was added to each tube ...
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bioRxiv - Microbiology 2022Quote: ... A 9:1 ratio of 10X DNase I buffer (Invitrogen) was added to each aliquot ...
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bioRxiv - Neuroscience 2024Quote: ... 9:1 in 10x HBSS (Gibco/Thermo Fischer Scientific, U.S.) and 35% SIP was prepared by diluting 100% SIP 0.35:1 in 1x HBSS pH 7.0-7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... grown to confluence and incubated with the calcium indicator Fluo 4-AM (Fluo-4 Direct assay kit, Invitrogen) for 60 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... external cysteine residues were blocked with 107 μM 4-acetamino-4’-maleimidylstilbene-2,2’-disulfonic acid (AMS, Thermo Scientific) for 20 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... or separating the pooled samples on a 4% agarose gel (E-Gel® EX Agarose Gels, 4%, Invitrogen), and gel purification of fragments larger than adapter dimers (>150bp) ...
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bioRxiv - Neuroscience 2021Quote: ... Brain halves were either drop-fixed in phosphate buffered 4% paraformaldehyde 4°C (Thermo Fisher Scientific, Waltham, MA) for 24 hours at for immunohistochemical staining or micro-dissected (cortex ...
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bioRxiv - Immunology 2024Quote: ... The tissues were fixed overnight at 4 °C in 4% methanol-free paraformaldehyde (ThermoFisher Scientific, cat no. U01H501), and then washed three times with 1xPBS for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of 100 μl of sample was loaded onto 4-12% Novex Tris-glycine gel (Invitrogen, XP04120BOX). Western blotting was done using an anti-FLAG antibody ...
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bioRxiv - Bioengineering 2023Quote: ... Fluo-4 reagent solution (prepared according to manufacturer’s instructions; Fluo-4 Direct Calcium-Assay-Kit, Thermo Fisher Scientific) was added to culture medium (50% of culture medium volume ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 2min at 85C and loaded on a TrisGlycine 4-20% SDS-PAGE gel (4-20%, Invitrogen) along with a PrecisionPlus Kaleidoscope protein ladder (BioRad 1610375) ...
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bioRxiv - Neuroscience 2022Quote: ... A Neuropixels probe (option 3 phase A) with 374 recording sites was briefly (∼2 minutes) dipped into the diI cell-labeling solution (ThermoFisher) to visualize probe tracks ...
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bioRxiv - Molecular Biology 2020Quote: ... medium from the basolateral compartment was renewed every 2–3 days and the apical surface was washed once a week with PBS (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: Bone marrow was obtained from 2-3-month-old mice and cultured with 100 ng/ml of M-CSF (Prospec Bio) in αMEM (Gibco Invitrogen) supplemented with 10% FCS for three days ...
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bioRxiv - Genetics 2019Quote: ... Transfection was performed using 2 μg plasmid DNA and 3 μL Lipofectamine in 150 μL Opti-MEM I (ThermoFisher 31985062). At collection ...
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bioRxiv - Systems Biology 2019Quote: ... the media was aspirated and replaced with 100μL phenol-red free RPMI-1640 and 12mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494) was added to the cells ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were desalted on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. × 2 cm; Thermo Scientific) using a mobile phase of 0.05 % (v/v ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Genetics 2021Quote: 2-3 × 106 mESCs were transfected with 14.5 μg of TALE plasmid and 26 μL Lipofectamine 3000 Reagent (Invitrogen L3000015) and seeded onto 0.1% gelatin-coated 10 cm dish containing an autoclaved SuperFrost Plus Adhesion glass slide ...
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bioRxiv - Bioengineering 2020Quote: ... proteins and the Canadian clinical isolate of SARS-CoV-2 (V-2/Canada/VIDO-01/2027 Feb 2020) were mixed in fresh media supplemented with 3% fetal bovine serum (Gibco) and preincubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS prior to loading with 3 μM of the Ca2+ indicator Fura-2 AM (Invitrogen) diluted in a modified Krebs-Ringer buffer [KRBH ...
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bioRxiv - Pathology 2021Quote: ... The tissue was homogenized with a Pasteur pipette in Growth medium [3 ml DMEM-F12 with 2% of B27 without vitamin A (Gibco), 20 ng.mL-1 Epidermal Growth Factor (EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018) using a dT primer and Superscript IV (Invitrogen) reverse transcriptase ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were loaded with 3 μM Fura-2-AM and 0.01% (v/v) Pluronic F-129 (both from Molecular Probes) in DMEM medium at 37 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Bioengineering 2022Quote: ... The medium was replaced every 2-3 days and cells were split once they reached 75-80% confluence using TrypLE Express (Gibco). LNCaP and U87 cells were gifted by Professor Lisa Butler and Professor Stuart Pitson ...
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bioRxiv - Molecular Biology 2019Quote: ... 3’-linked footprints were denatured at 80°C for 2 min and purified on 10% TBE-Urea polyacrylamide gels (Invitrogen) in 1x TBE (Ambion ...
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bioRxiv - Bioengineering 2021Quote: ... scaffold sequences and 3’ extension sequences were amplified with indicated primers (Supplementary table 2) using Phusion master mix (ThermoFisher Scientifc) or Q5 High-Fidelity enzyme (NewEnglandBioLabs) ...
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bioRxiv - Immunology 2019Quote: ... isolated lungs were cut into 2-3 mm2 sections and resuspended in 5 mL digestion solution of Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco) supplemented with 5 mM GlutaMax (Gibco) ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ZO-2 pAb (38-9100) and rabbit anti-ZO-3 pAb (36-4100) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were regularly monitored for bacterial contamination by checking for bacterial growth in Zobell marine broth 2216 (HiMedia) (71) in addition to filtering 2-3 mL of exponential-phase growing culture and using Sybr Green I (Invitrogen) staining and epifluorescence microscopy (Nikon Eclipse 80i ...
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bioRxiv - Molecular Biology 2021Quote: ... For cell collection and/amplification ESCs were trypsinized for 2 to 3 minutes with trypsin-EDTA (Invitrogen, Cat#25200-072) and the digestion was stopped by the addition of pre-warmed 15% FCS ...
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bioRxiv - Immunology 2020Quote: ... 2 and 3 cDNA were amplified by real-time PCR using the SYBR Green PCR kit (Thermo Scientific, Braunschweig, Germany). The primer sequences used for the real-time PCR were listed in Table 1.
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bioRxiv - Cancer Biology 2022Quote: ... Panel 2: (PE-Cf594 Ly6G, PerCP-Cy5.5 Ly6C, FITC CD11b) and Panel 3: Alexa fluor 594 anti-mCherry (ThermoFisher-M11240), Alexa fluor 647 CD34 (BD 560230) ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 10 μg protein and BSA for the negative control were denatured at 70°C for 10 min with LDS buffer and reducing agent (Novex) and migrated with a 3 to 198 kDa molecular weight marker (SeeBlue plus 2, Invitrogen). A gel-sized PVDF membrane (Amersham ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured by MTT assay using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide kit (Thermo-Fisher Scientific M6494). For the isobologram assays ...