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Citations for Thermo Fisher :
4301 - 4350 of 7736 citations for Recombinant Human C1QB His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
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bioRxiv - Genetics 2019Quote: ... Thirty-two libraries of 100 pM were mixed together and emulsion PCR amplified on the Ion PI™ Hi-Q™ ISPs using the Ion OneTouch™ 2 Instrument (Life Technologies, Carlsbad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Template preparation was performed by the Ion PGM Hi-Q View kit on the Ion OneTouch™ 2 System (Life Technologies, Grand Island, NY, United States) and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes and melanoma cells were lysed in Pierce RIPA buffer (Thermo Scientific) containing 1× Halt protease inhibitor cocktail (100× ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CRC organoids medium is composed of Ad-DF+++ (Advanced DMEM/F12 (GIBCO) supplemented with 2 mM Ultraglutamine I (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Gibco (Cat#: C0035C) and cultured in Medium 200 (Cat# ...
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bioRxiv - Cell Biology 2020Quote: ... HIF1A levels were measured in duplicates applying a HIF1A Human ELISA kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Cell Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Gibco, #A3349401) and passaged with EDTA (Life Technologies #15575-020) ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
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bioRxiv - Genetics 2019Quote: ... RPE1 cells and primary human fibroblasts were cultivated in complete DMEM medium (Gibco).
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bioRxiv - Molecular Biology 2021Quote: Human MSCs were maintained in growth media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing D-glucose (1g/L) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher). Templating and sequencing were performed with the Ion PI HiQ OT200 kit ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Perforin eF450 (1:100, clone dG9, Invitrogen, cat # 48-9994-42). Cells were analyzed on a BD LSR Fortessa flow cytometer ...
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bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
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bioRxiv - Immunology 2021Quote: After 48 h of stimulation with Human T-activator CD3/CD28 Dynabeads (ThermoFisher) CD4+ lymphoblasts were recovered for transfection of CRISPR/Cas9 nucleoprotein complexes ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and 10 µl/ml Fibronectin from human plasma (Thermo Fisher Scientific, #33016-015)] for 2-3 h at 37 °C/5% CO2 and stored at 4 °C after thorough removal of coating solution.
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bioRxiv - Physiology 2019Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: We used GeneChip Human Exon 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) to measure the expression levels of transcripts in each brain tissue specimen ...
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bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse and human PSCs were cultured in DMEM (10-013-CV; Fisher Scientific) containing 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... and sCD163 (Thermo Scientific™ Pierce™ Human CD163 Kit, Thermo Scientific, USA) in plasma.
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bioRxiv - Immunology 2020Quote: ... Human embryonic kidney (HEK293T) cells and HeLa cells were cultured in DMEM (Gibco) supplemented with 100U/ml Penicillin (Sigma-Aldrich),100μg/ml Streptomycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... T Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco). The cells were cultured in GRex (Wilson Wolfe ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Cells were incubated with AF488 goat anti-human IgG1 (1:500, A10631, Invitrogen) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Amyloid Beta-40 ELISA was performed using the Human AB40 ELISA Kit (Invitrogen). Secretion values were normalized to protein content of wells as measured by RIPA harvest and BCA protein quantification.
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bioRxiv - Immunology 2020Quote: ... T cells were stimulated with Dynabeads® Human T expander CD3/CD28 (Invitrogen) at a 1:3 ratio (T cell:bead) ...
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bioRxiv - Genomics 2019Quote: Human colon carcinoma cells HT-29 were cultured in 1640 medium (Gibco, USA) supplemented with 10% (v/v ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells stained with goat anti-human IgG Alexa Fluor 647 (Life Technologies, USA) and Donkey anti-human IgM Alexa FITC (Southern Biotech ...
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bioRxiv - Immunology 2019Quote: ... anti-human CD8 conjugated to Pacific Blue (clone 3B5, cat. no. MHCD0828, Invitrogen), anti-human CD39 conjugated to APC (clone A1 ...
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bioRxiv - Cell Biology 2019Quote: ... goat anti-human Alexa-555 (1:1000, Cat. #A-21433, Thermo Fisher Scientific), donkey anti-mouse Alexa-488 (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... 5% heat-inactivated AB human serum and 20 ng/mL M-CSF (Invitrogen). Cells were washed with PBS and resuspended in fresh media on days 3 and 6 of differentiation ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK293) cells were maintained in Dulbecco’s modified eagle medium (Gibco) containing 10% fetal bovine serum (HyClone) ...
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bioRxiv - Cancer Biology 2019Quote: Human RPE1-hTERT cell lines were grown in DMEM+GlutaMAX-I (Gibco, 10569) supplemented with 10% FBS and 1% Pen Strep (100 U/ml) ...
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bioRxiv - Genetics 2021Quote: Wild-type human FAN1 gene was cloned in a derivative of pFASTBAC1 (Invitrogen) with GST- ...
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bioRxiv - Genomics 2020Quote: ... 5% A+ human serum (Interstate Blood-Bank) and 0.5% AlbuMAX II (Life Technologies), 5% HC ...
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bioRxiv - Cell Biology 2021Quote: ... human bone marrow aspirates were diluted in phosphate buffered saline (PBS, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The sensor was then pulsed with increasing concentrations of purified human APC (ThermoFisher) in 20 mM Hepes pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... and human lamin were constructed in a U6 Block-it vector (Life Technologies). Adenoviruses expressing these shRNAs were established as per the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Physiology 2020Quote: ... M1 and M2 primary human skin fibroblasts were maintained in MEM (Fisher Scientific) supplemented with 20% fetal bovine serum and 2% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Human ES cells were maintained in culture with StemFlex culture media (Gibco A3349401) on growth factor reduced Matrigel coated dishes (Corning).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and the cells were stained with anti-human tau monoclonal antibody HT7 (Invitrogen) at 1:1,000 dilution in blocking buffer overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Human dermal fibroblasts used for iPS and hepatocyte generation were purchased from Invitrogen and human primary hepatocytes from KaLy-Cell ...
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bioRxiv - Neuroscience 2020Quote: The GeneChip® Human Gene 1.0 ST Arrays (Affymetrix®, Santa Clara CA) were used ...
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bioRxiv - Pathology 2021Quote: ... 5 μg/ml Alexa Fluor 647–conjugated goat anti-human IgM (Invitrogen, A21215) and PE–labeled anti-human IgD (used at a 1:40 dilution ...