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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... was used for 1h blocking and cells were incubated with anti-myc tag primary antibody (MA1-21316, Invitrogen) diluted 1:500 for 2h in RT ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in secondary antibody diluted in blocking buffer [goat anti-mouse 488 1:4000 (ThermoFisher Scientific A11029)] for 30 minutes at room temperature with orbital shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then incubated overnight at 4 ºC in blocking buffer containing 10% Goat Serum (Thermo Fisher) and 0.01% Triton X-100 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for BrdU staining: 2 mL blocking buffer containing 20 μL BrdU monoclonal antibody (Invitrogen, B35128). Secondary antibody solution for BrdU staining ...
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bioRxiv - Bioengineering 2024Quote: ... and anti-acetylated tubulin staining: 4 mL blocking buffer containing 8 μL Alexa Fluor 488 (Invitrogen, A-11006), 8 μL Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: Collected protein flow-throughs (FT) were subjected to protein precipitation for downstream protein content determination using bicinchoninic acid assay (BCA, Thermo Fisher) assay and trypsin protein digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
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bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
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bioRxiv - Neuroscience 2019Quote: ... Newly synthesized proteins were then enriched using the Click-it protein enrichment kit for chemistry capture of azide modified proteins (Invitrogen C10416) following the manufactures protocol with small modifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was performed according to Culturex protocol while protein purification was done using Pierce Protein A Agarose kit (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations across all samples were normalised to the same protein concentration (500 µg/ml) with protein determination quantified using the well-established Pierce BCA Protein Assay (Thermo Fisher) following the recommended guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
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bioRxiv - Genomics 2020Quote: ... 57°C for 30 sec and 72°C for 30 sec using AmpliTaq Gold® Polymerase (Applied Biosystems). The 7550-7555 product was DNA sequenced by Sanger technology at Eurofins Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Cryosections were made at −20°C to −16°C using a Cryostar NX (Thermo Scientific, Waltham, MA, USA) disinfected with Leica Cryofect Desinfectant Spray (Leica ...
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bioRxiv - Neuroscience 2020Quote: P4 mice were injected with anterograde tracers (CTB-488 and CTB-594, ThermoFisher Scientific (C-22841, C-22842)) in the left and right eyes ...
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bioRxiv - Microbiology 2022Quote: ... with 40 cycles of 15 s at 95°C and 1 min at 61°C (Applied Biosystems 7500). Primers used in this study are listed in Table S2.
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bioRxiv - Microbiology 2020Quote: ... the primary substrates were 24.7 ± 0.5 mM fructose (148.2 ± 3.2 mmol C L−1) and 18.7 ± 1.0 mM Na-butyrate (112.2 ± 6.3 mmol C L−1) (ThermoFisher, Kandel, Germany). For the pH experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... and digested at 37°C for three hours using 1:200 w:w endoproteinase Lys-C (Thermo Fisher Scientific). The urea was then diluted to 1.5 M and samples further digested at 37°C overnight with 1:25 w:w trypsin (Thermo Fisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We stored brains at −80°C until sectioned coronally on a cryostat (Cryostar NX50, Thermo Fisher; −21°C) and mounted slices directly onto slides in two alternating series ...
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bioRxiv - Biochemistry 2021Quote: ... including 7992 sequences) appended with the iRT peptide sequence using Proteome Discoverer software (version 2.1, Thermo Scientific). The search parameters were set as follows ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were measured on an EASY-nLC 1200 ultra◻high◻pressure system (Thermo Fisher Scientific) coupled to a quadrupole Orbitrap mass spectrometer (Q Exactive HF and HFX and Exploris 480 ...
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bioRxiv - Systems Biology 2020Quote: Peptide extracts (1 μg on column) were separated on an Ultimate 3000 RSLC nano system (Thermo Scientific) using a PepMap C18 EASY-Spray LC column ...
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bioRxiv - Cell Biology 2020Quote: ... for peptide separation coupled online to a Q-Exactive Quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Waltham, Massachusetts) for MS/MS acquisition ...
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bioRxiv - Cell Biology 2020Quote: ... ~4 mg of peptides were enriched for phosphopeptides using a Fe-NTA phosphopeptide enrichment kit (Thermo Scientific) according to instructions provided by the manufacture and desalted ...
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bioRxiv - Developmental Biology 2021Quote: ... peptides were separated using revere phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The peptide sequences were verified by us using a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). HEK293 and Sf21 cells expressing FLAG-TRPR were first seeded onto poly-L-lysine-coated 96-well plates and cultured overnight ...
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bioRxiv - Neuroscience 2021Quote: 8 TMT reagents from a 10-plex reagent kit were used to label desalted peptides (Thermo Fisher) as directed by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluting peptides were on-line sprayed in a LTQ XL mass spectrometer (Thermo Scientific, Sacramento, US). Full scan mass spectra were collected in the linear ion trap in the mass range of m/z 350 to m/z 1800 Da and the 10 most intense precursor ions were selected for collision-induced fragmentation ...
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bioRxiv - Neuroscience 2021Quote: ... and an aqueous phase containing peptides was collected and dried with a SpeedVac (Thermo Fisher Scientific, U.S.A.).
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bioRxiv - Molecular Biology 2021Quote: Eluting peptides were analyzed in data-dependent acquisition mode on a Fusion Lumos mass spectrometer (ThermoFisher Scientific) coupled to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were purified using a 2-µl bed volume of Poros 50 R2 (Applied Biosystems) reversed-phase beads packed in Eppendorf gel-loading tips (Erdjument-Bromage et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The mass spectra were collected using the “Universal” method optimized for peptide analysis provided by Thermo Scientific. Full MS scans (375–1500 m/z range ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were analysed by nanoLC-MS/MS on a Q Exactive HF-X mass spectrometer (Thermo Scientific) as described previously with the following changes ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled samples were fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were analysed by nano-LC MS/MS using an Ultimate 3000 LC system (Dionex, Thermo Scientific) coupled to the triple TOF 5600 mass spectrometer (Sciex) ...
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bioRxiv - Immunology 2021Quote: ... Peptides were trapped on a pre-column (PepMap100 C18 3 μm; 75μm x 2cm, Thermo Fisher Scientific) and separated on an EASY-Spray column (ES803 ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides were loaded onto an Acclaim Pepmap 100 trap column (75 µm ID × 25 cm, Thermo Scientific) for 10 min at 3 µLs/min then eluted onto an Acclaim PepMap RSLC analytical column (75 µm ID × 25 cm ...