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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and 10 µM each of primer (total volume: 10 µL, Invitrogen). We ran plates on a CFX384 Touch Real-Time PCR detection system (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... 10 u/ml penicillin and 10 μg/ml streptomycin (Thermofisher 15140122) and 1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... 10 u/ml penicillin and 10 µg/ml streptomycin (Thermofisher 15140122) and 0.1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... with 10% of heat-inactivated FBS (#10-438-026; Thermo Fisher) and 1x Penicillin-Streptomycin solution (#15-140-122 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% dialyzed FBS (MWCO 10 kDa, Thermo Fisher Scientific), 100 U/ml of penicillin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cat#41061-029)containing 10% fetal bovine serum (FBS, Gibco; cat# 10-438-034), 100LU/mL of penicillin-streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 000 U/ml penicillin and 10 000μg/ml streptomycin (Gibco). The cell lines were cultured and maintained at 37°C with CO2 level of 5% in a humidified atmosphere.
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bioRxiv - Developmental Biology 2023Quote: ... 10 μl of 10 mM EdU (Thermo Fisher Scientific, Cat #: C10640) was injected intraperitoneally in the region between the pelvic fins ...
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bioRxiv - Pathology 2024Quote: ... and 10 μl of RNAse A (10 µg/µl) (Thermo Scientific) followed by incubation for 45 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: The proteolytic activity of activated globupain and its substitution variants was assessed using the casein gelzan™ CM plate assay and the EnzChek™ Protease Assay Kit (Thermo Fisher Scientific, MA, USA), respectively ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from 8/16-cell embryos (10-15/group/replicate) was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). Purified RNA was then subject to reverse transcription with SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... plus the Renal Epithelial Cell Growth Kit (ATCC) and mesenchymal cell medium consisting of DMEM high glucose with 10% FBS (Life Technologies), 2mM GlutaMAX-I (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... Amplicon cloning was conducted with TOPO TA Cloning Kit One Shot Top 10 Escherichia coli following the manufacturer’s protocol (Invitrogen, CA, USA). All fragments were Sanger sequenced to verify on-target cloning and directionality (Table S6) ...
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bioRxiv - Systems Biology 2020Quote: ... Peptides from each individual case (n=400) and the GIS pooled standard (n=100) were labeled using the TMT 10-plex kit (ThermoFisher 90406). In each batch ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Genetics 2019Quote: ... the PCR products in the range from 350 bp to 10 kb were cut out and extracted from the gel (GeneJet Gel Extraction Kit, Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... typically 50-100 pmol sgRNA was delivered into 2*105 cells per electroporation using the Neon 10 µL Kit (Invitrogen, #MPK1096) or 500-1000 pmol of sgRNA delivered into 2*106 cells per electroporation using the Neon 100 µL Kit (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides from each individual case and the GIS pooled standard or bridging sample (at least 1 per batch) were labeled using the TMT 10-plex kit (ThermoFisher 90406) for ROSMAP BA9 tissues ...
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bioRxiv - Microbiology 2021Quote: ... 400 µL clear aqueous phase was aspirated from the top layer of the plate and transferred to a deep well plate containing 200 µL 100% isopropanol and 10 µL RNA binding beads from the MagMAX-96 for Microarrays Total RNA Isolation Kit (ThermoFisher, #AM1839). The Nanocell system then transferred this plate to a connected KingFisher Flex ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA quantity and quality was validated using the Nanodrop spectrophotometer and 10 ng of total RNA was used to synthesise cDNA using the TaqMan Advanced miRNA cDNA Synthesis Kit (Applied Biosystems). miRNA expression of hsa-miR-21-5p ...
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bioRxiv - Neuroscience 2020Quote: ... to magnetic beads at a final concentration of 10 mg of antibody/ mL of beads was done following the Magnetic Dynabeads Antibody Coupling Kit from Invitrogen (14311D). Human tissue lysates were also prepared using the “Sequential Extraction of Proteins from Tissue” protocol as described above with addition of 10 mM iodoacetamide prior to homogenization ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and transfected into GFI1-3×FLAG CCRF-CEM cells by electroporation using the Neon transfection system 10 μL kit (ThermoFisher Scientific). Electroporation parameters were 1500V ...
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bioRxiv - Cell Biology 2021Quote: ... and processed independently according to the manufacturer’s protocol for a 10-plex Tandem Mass Tag (TMT) reagent labeling kit (Thermo Fisher Scientific). The different TMT-labeled peptide mixtures were pooled equally ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Microbiology 2021Quote: ... fifty microlitre of supernatant of 10% tissue homogenates from relevant ferrets was utilised by using the MagMax 96 Viral RNA Kit (ThermoFisher Scientific) in a MagMAX Express Magnetic Particle Processor (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated using High-capacity cDNA Reverse Transcription Kit in a 20 μl reaction volume and diluted 1:10 for qPCR analysis (Life Technologies). qPCR analysis was performed using 1 μl diluted cDNA with biological (2-3 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100 with 100× protease inhibitors cocktail solution from GenDEPOT [P3100-005] and the phosphatase inhibitors NaF [10 mM] and Na3VO4 [20 mM]) and quantified using a bicinchoninic acid (BCA) protein assay kit (#23225, Thermo Scientific). The samples were added with 6X loading dye and boiled at 97 °C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: Immature leaves collected from pSPCH::SPCH-GFP of 21-day-old plants were cross-linked using 1% formaldehyde under vacuum for 10 min according to the EZ-ChIP chromatin IP kit protocol (Thermo Scientific). After washing with phosphate-buffered saline solution ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Microbiology 2022Quote: ... Lysate from the beads was clarified by centrifugation at 20,000 × g for 10 min and quantified using Pierce 660-nm protein assay kit (Thermo Fisher Scientific). Ten micrograms of protein was run per sample on 4–15% Mini-Protean TGX stain-free precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Ex vivo S phase entry was monitored by supplementing the media with 10 µM EdU and staining using Click-iT™ EdU Cell Proliferation Kit (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... The glass capillary containing the cell was then broken into a sterile Eppendorf tube containing 10 μL of lysis buffer with DNAse (TaqMan Gene Expression Cells-to-CT Kit; Invitrogen, #4399002). A bath control was also taken for each coverslip of cells and analysed concurrently with cells ...
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bioRxiv - Microbiology 2021Quote: ... reverse transcription mixtures contained 1 µg or 10 µl RNA and High-Capacity RNA-to-cDNA reverse transcription kit (Applied Biosystems). qRT-PCR mixtures were assembled using 50 ng or 5 µl cDNA and PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... The ATP content from 10 μL aliquots of lysed cells was then detected using a luminescence-based ATP determination kit from Life Technologies and ATP levels were calculated by extrapolation from a calibration curve constructed with known concentrations of ATP ...
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bioRxiv - Systems Biology 2022Quote: A final volume of 10 µL of purified mRNA was obtained using the Dynabeads® mRNA Direct Kit (ThermoFisher, Cat#61012) from an optimized protocol to work in 96-well plates 42.
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bioRxiv - Immunology 2022Quote: ... The 8-10 μm tissue sections were cut with cryostat (NX70 MicromMicrotech) and we used the Tyramide SuperBoost™ kit (Invitrogen) according to the manufacturer’s instructions for immunofluorescence staining ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μl diluted RT product was applied to each 10 μl qPCR reaction using the TaqMan Universal Amplification kit (ThermoFisher/AppliedBiosystems) with customized TaqMan probes (Hs.PT.58.21450367 for TCF4 ...
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bioRxiv - Cell Biology 2019Quote: ... HBSS apically) containing 10 µM EdU (Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit, C10645, Invitrogen) was perfused through the apical and basal channels of the chip for 18h prior to cell harvest ...
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bioRxiv - Physiology 2020Quote: ... Membranes was washed again with TBST for 10 min x 3 times and revealed using the Super Signal West Pico kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... GBM cells in ultrapure water were then sonicated for 10 minutes and used for dsDNA quantification using the Quant-iT PicoGreen dsDNA kit (Molecular Probes, Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total RNA was isolated from a composite sample of 10 individual F0 or F1 flies (five males and five females) by use of an RNA extraction kit (Thermo scientific), then cDNA was synthesized from RNA extracts according to the manufacturer’s protocol (see Al Naggar et al. ...
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bioRxiv - Neuroscience 2020Quote: ... to magnetic beads at a final concentration of 10 mg of antibody / mL of beads was done following the Magnetic Dynabeads Antibody Coupling Kit from Invitrogen (14311D). Human tissue lysates were also prepared using the “Sequential Extraction of Proteins from Tissue” protocol as described above with addition of 10 mM iodoacetamide prior to homogenization ...
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bioRxiv - Genetics 2020Quote: AAG AGC TCT TCA GAA ATG GAT GCC ATG TGT GTC TCC TCT TCC TGA-3’) derived from the intron 10 of PIK3C2B were biotin-labeled using Biotin 3’-End DNA Labeling Kit (Thermo Scientific). Biotin-labeled ssODNs were then annealed with reverse complimentary ssODNs to generate biotin-labeled probes ...
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bioRxiv - Microbiology 2021Quote: Eukaryotic RNA (18S and 28S rRNAs and polyadenylated RNAs) was removed from total RNA (10-25 μg) extracted from infected tissues by MICROBEnrich™ kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then fixed in 4 % PFA for 10 minutes and stained using Click-iT Edu Alexa Fluor 594 Imaging kit (Life Technologies) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pulsed-labelled with 10 μM EdU (Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit, Thermo Fisher Scientific) in fresh culture medium for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The samples (N=400) and the pooled global internal standards (GIS) (N=100) were labelled using the TMT 10-plex kit (ThermoFisher 90406) as previously described in (Johnson et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then fixed in 4% PFA for 10 min at room temperature and stained with the Click-iT EdU Alexa Fluor 647 assay kit (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Peptides from each individual (n=400) and the GIS pooled standard (n=100) were labeled using the TMT 10-plex kit (ThermoFisher 90406). Peptide eluents were separated on a self-packed C18 (1.9 μm ...
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bioRxiv - Plant Biology 2022Quote: ... from Arabidopsis gDNA or cDNA of 10-day-old seedlings and cloned into the pENTR/D-TOPO vector using the pENTR TOPO cloning kit (Life Technologies) and subsequently cloned into Gateway® destination vector pBGW,0 (pTTM1:TTM1 ...