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Citations for Thermo Fisher :
4201 - 4250 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... after which cells were stained for 1 hour at room temperature with AlexaFluor-conjugated secondary antibodies diluted in PBS+10% FCS (Life technologies) and stained with DAPI ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Developmental Biology 2023Quote: ... sorted CD31-CD45-CD184+ cells were cultured in complete growth medium DMEM supplemented with 20% FCS and 1% penicillin/streptomycin (Gibco, 15140122).
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bioRxiv - Immunology 2023Quote: ... in PBS for 10 min prior to addition of the relevant surface marker fluorochrome-conjugated antibodies in FACS buffer (PBS, 2% heat-inactivated FCS) supplemented with Super Bright staining buffer (Thermo Fisher). Following 30 min of incubation ...
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bioRxiv - Bioengineering 2023Quote: ... TF-1 were purchased from DSMZ (Cat#ACC334) and maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies), 2mM GlutaMAX and 2ng/ml hGM-CSF (215-GM ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). The MA104/NSP5-BioID2 cell line was generated using a lentiviral system ...
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bioRxiv - Immunology 2023Quote: ... were performed according to the manufacturer’s instructions with 50 000 cells per well and read at 492nm on a microplate absorbance reader (Multiskan FC microplate photometer, Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: Splenic B cells were resuspended at a concentration of 5 × 106 cell/ml in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (Thermo Fisher, #F14201) and 3 μM Fura Red (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Purified Fc-FGF-21CT was subject to an endotoxin removal proces (Pierce High Capacity Endotoxin Removal Spin Columns, Thermo Fisher Scientific) before flash-frozen and stored at -80°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD-CD45-CD235A-CD71- non-hematopoietic fraction was sorted in DMEM supplemented with 10% FCS for Single-cell RNA sequencing and 7AAD-CD45-CD235A-CD71-CD31-CD274+ BMSCs were sorted in TRIzol (Life Technologies) for bulk RNA sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; PAN-Biotech, Aidenbach, Germany, #P30-3306) and penicillin–streptomycin (100 U/ml; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, PAA Laboratories GmbH, Colbe, Germany) containing 10% fetal calf serum (FCS, Invitrogen, Carlsbad, California, USA), and 2 mM L-glutamine (PAA laboratories GmbH ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped by adding 100 µL H2SO4 (0.5 M) to each well and absorbances were measured in a microplate reader (Multiskan FC; Thermo Fisher Scientific) at 450 nm and 620 nm.
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (4 x 300 µL PBST) and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat ...
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bioRxiv - Immunology 2024Quote: ... we cultivated the corresponding rat hybridomas in IMDM (Gibco, 12440-053; supplemented with 10 % FCS, 1X MEM Non-essential amino acid solution (ThermoFisher, 11140050), 2 mM Glutamax (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for one hour in primary antibody NPM1 (FC-61991, ThermoFisher, 1 : 500 dilution in PBST with 1 %BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... and Rspo3-Fc Fusion Protein Conditioned Medium (Immunoprecise, #R001-100ml, 2%)] and mixed with 50 µl Matrigel (Fisher Scientific, #CB-40230C). Human alveolar organoid media was refreshed every 3 days until the end of the experiment ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). MA/cytBirA (24 ...
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bioRxiv - Immunology 2024Quote: ... placed static for 1 min at RT after which the absorbance at 450 nm was measured using the Multiskan FC Microplate Photometer (Thermo Scientific). A measurement was taken every 2 minutes for 50 minutes ...
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bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD14 (11-0149-42, Thermo Fisher Scientific, 1:100), anti-human CD117 known also as cKIT (12-1178-42 ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Microbiology 2021Quote: ... or a type IV DQTM collagen conjugate from human placenta (Invitrogen). Reactions were prepared in technical triplicate or quadruplicate by mixing 20 µL of substrate at 0.25 mg/mL with 80 µL of reaction buffer and 100 µL of bacterial suspension ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human mammary epithelial cells (HuMECs) were provided by Thermo Fisher Scientific and cultured according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: Cultured primary human airway epithelial cells were washed with PBS (Gibco), fixed with 4% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Microarray analysis was performed with the Human Gene 1.0ST Array (Affymetrix). Protein expression was analyzed by Simple Western (ProteinSimple) ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Immunology 2021Quote: ... efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific, MA, USA), FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... We quantified expression using the IgG4 Human ELISA kit (Invitrogen BMS2095) with IgG4 standards provided in the kit ...
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bioRxiv - Genetics 2021Quote: Human TERT cDNA39 was cloned into the gateway pDONR221 plasmid (Invitrogen). TERT variants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... human testicular cells were suspended in MEMα medium (Thermo Fisher Scientific) containing 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Human ESCs were cryopreserved in PSC Cryomedium (Gibco, Cat. No. A2644601). The culturing medium was supplemented with 1X RevitaCell (100X ...
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bioRxiv - Cell Biology 2022Quote: ... Human NGFR siRNA or negative control siRNA were obtained from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... human neurons were transfected with Lipofectamine 3000 (#L3000015, Thermo Fisher Scientific) and 1.6 μg of plasmid DNA at 40 - 50 DIV ...
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bioRxiv - Neuroscience 2021Quote: ... Primary human and NHP microglia were cultured in DMEM/F12 (Gibco) supplemented with 10% defined fetal bovine serum (HyClone) ...
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bioRxiv - Genomics 2020Quote: ... 20x human TERT TaqMan™ Copy Number Reference Assay (ThermoFisher 4403316) as internal reference control ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...
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bioRxiv - Cancer Biology 2019Quote: ... human 36B4 RT 5′-CCCATTCTATCATCAACGGGTACAA-3′) using Superscript III (Thermo Fisher) at 55 °C for 1 h ...
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bioRxiv - Bioengineering 2019Quote: Human primary dermal fibroblasts derived from adult female forearm (ThermoFisher, C0135C) were cultured in DMEM-High Glucose with Glutamax (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: The human C10orf76 gene (Uniprot Q5T2E6) was synthesized by GeneArt (Thermofisher). c10orf76 and PI4KB (Uniprot Q9UBF8-2 ...
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bioRxiv - Genomics 2019Quote: Proliferating human myoblasts were cultured in F10 medium (Gibco/Life Technologies) supplemented with 20% fetal bovine serum (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: Proliferating human myoblasts were cultured in F10 medium (Gibco/Life Technologies) supplemented with 20% fetal bovine serum (Thermo Scientific) ...