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Citations for Thermo Fisher :
4201 - 4250 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: iPSCs with a stably integrated human NGN2 were single-cell dissociated using Accutase (Thermo Scientific) and plated at a concentration of 1.5×106 per well of a Matrigel-coated (1:100 ...
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bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Bioengineering 2022Quote: ... at a dilution 1:2000 and rabbit anti-OCN antibody (Reactivity: human, Invitrogen, PA5-96529) at a dilution 1:100 in 1% BSA in PBS-T for 1h00 at room temperature ...
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bioRxiv - Immunology 2022Quote: ... for viability and antibodies directed against human CD19 (APC clone SJ25-C1, Thermo Fisher Scientific), CD38 (PE-Cy7 clone HB-7 ...
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bioRxiv - Neuroscience 2024Quote: ... Human IL-1β was measured by IL-1β kit (Thermo Fisher, catalog no. 88-7261). Human Aβ42 was measured by Aβ42 kit (ExCell Bio ...
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bioRxiv - Biophysics 2024Quote: ... the full-length human AGO2 was cloned into a pFastBac dual expression vector (Thermo Fisher) containing an N-terminal His-tag followed by a TEV cleavage site and transformed into chemically competent DH10EmBacY cells (Geneva Biotech) ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
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bioRxiv - Immunology 2023Quote: ... in presence of Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, from Thermofisher, USA) and IL-2 (50 U/mL ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human primary monocytes and THP-1 cell lines (ATCC) were cultured in RPMI 1640 (Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Human Embryonic Kidney 293T (HEK) cells (ATCC) were maintained in high glucose DMEM media (Gibco). Media was supplemented with 10% FBS (Millipore Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Cultured 5637 human bladder cancer cells were lysed in Pierce IP Lysis Buffer (ThermoFisher, 87787), and incubated for 30mins at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA samples were isolated from human hippocampus and cerebellar tissues using TRIzole (Life Technologies), as we reported 68,69 ...
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bioRxiv - Neuroscience 2024Quote: ... for human β-globulin the mastermix was made using 5 μL Fast SYBR Green (ThermoFisher), 1 μL of hbg1 primer (3 μM) ...
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bioRxiv - Neuroscience 2024Quote: ELISA kits for human Aβ40 (Cat. #KHB3481) and Aβ42 (Cat. #KHB3544) were purchased from Invitrogen and FSH (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... plates were pretreated with 10 µg/mL human fibronectin (ThermoFisher Scientific, Cat. #33-016-015) at 37 °C for ≥1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturers’ instructions with the following TaqMan probes human VEGFA (Hs00900055_m1; Thermo Fisher Scientific), mouse VEGFA (Mm00437306_m1 ...
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bioRxiv - Genomics 2024Quote: Human iPSCs were cultured in Essential 8 Medium (Gibco/Thermo Fisher Scientific; Cat. No. A1517001) on 6-well plates coated with Matrigel Matrix (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney 293T (HEK293T) cells were grown in supplemented DMEM medium (Thermo Fisher Scientific). At 70% of confluence ...
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bioRxiv - Developmental Biology 2024Quote: ... 5iLA naïve human PSCs were dissociated into single cells by incubation with TrypLE (Thermo Fisher) for 3-5 min at 37°C ...
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bioRxiv - Genomics 2024Quote: Human myelogenous leukemia cells (K562) were cultured in RPMI 1640 medium (Gibco, catalog no. 11875093) supplemented with 10 % FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: Immortalized human epithelial bladder cells PD07i27 were maintained in antibiotic free growth EpiLife Medium (Gibco) supplemented with growth supplement (HKGS ...
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bioRxiv - Immunology 2024Quote: Human IgG (hIgG) was coupled to M-450 Epoxy Dynabeads (cat# 14011, Thermo Fisher Scientific) according to the product manual ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Immunology 2024Quote: ... to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L) (Invitrogen) to detect VHH-IgG Fcs ...
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bioRxiv - Cancer Biology 2024Quote: ... Human T cells were distinguished with live/dead viability stain (Thermo Fisher Scientific, Carlsbad, CA), followed by human CD3 and CD8 (clone OKT3 and clone SK1 ...
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bioRxiv - Bioengineering 2024Quote: ... and the resulting supernatant was assayed using a human albumin ELISA kit (ThermoFisher Scientific; EHALB) according to the manufacturer’s instruction.
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bioRxiv - Bioengineering 2024Quote: ... The isolated cells were then activated with magnetic anti-human CD3/CD28 dynabeads (CTS, ThermoFisher). Beads were mixed at a 1:1 ratio with cells and cells were cultured at a density of 1 x 106 cells/mL in complete X-Vivo 15 culture media (Lonza ...
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bioRxiv - Bioengineering 2024Quote: ... Human umbilical vein endothelial cells (HUVECs) were expanded in Endothelial Growth Medium 2 (Fisher Scientific) until confluent ...
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bioRxiv - Bioengineering 2024Quote: Primary human hepatocytes were transfected 1 day after thawing using Lipofectamine MessengerMAX Transfection Reagent (Invitrogen) using the following protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... internal controls (human Tert) and FGFR2 or MYC target gene probe (Thermo Fisher Scientific #4400292). Reactions were run on the QuantStudio 6 or 7 (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: Human neuroblastoma cells (SH-SY5Y) were cultured in DMEM/F-12 Glutamax™ (#10565018, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Bioengineering 2024Quote: ... The µAT platforms were incubated overnight with an mouse anti-human CD31 primary antibody (Invitrogen, Cat# MA5-13188 ...
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bioRxiv - Cell Biology 2023Quote: ... Keratinocytes were grown in M154 medium supplemented with human keratinocyte growth supplement (Thermo Fisher Scientific), 1,000 x gentamycin/amphotericin B solution (Thermo Fisher Scientific) ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Synthetic Silencer Select siRNAs against human NFATC4 (Thermo Fisher Scientific, Assay ID-s9484 and s9483), siRNAs against YBX1 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: IFN-γ ELISpots were performed using Human IFN gamma ELISpot KIT (Invitrogen, San Diego, CA) and MultiScreen Immobilon-P 96 well filtration plates (EMD Millipore ...
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bioRxiv - Neuroscience 2022Quote: Random primers were used to construct a cDNA library from human foetal brain mRNA (Invitrogen) into the pP6 plasmid derived from the original pACT2 ...
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bioRxiv - Molecular Biology 2022Quote: The immortalized human mammary epithelial cell line (HMLE) was cultured in HuMEC Ready Media (ThermoFisher) with HuMEC Supplement Kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: Human fibroblasts were obtained from the Coriell cell repositories and cultured in DMEM (Gibco, 11960085) with 10% FBS (Hyclone ...
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bioRxiv - Bioengineering 2022Quote: Voltage activity within human heart organoids was assessed using di-8-ANEPPS (Thermo Fisher Scientific). Di-8-ANEPPS was solubilized in DMSO per the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived neurons (differentiation protocol 1) were harvested in TRIzol reagent (Invitrogen #15596026). Following TRIzol/chloroform extraction ...
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bioRxiv - Cancer Biology 2023Quote: RNA samples were hybridized on ClariomTM D (human) microarrays (Thermo Fisher Scientific, Waltham, MA, USA) and data preprocessed using standard Bioconductor (RRID:SCR_006442 ...
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bioRxiv - Microbiology 2023Quote: HEK293T human embryonic kidney cells were cultured in Dulbecco’s modified essential medium (DMEM) (Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: HepG2 human hepatoma cells were cultured in Dulbeco’s Modified Eagle Medium (DMEM) (Gibco 10313-021) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... T cells were activated with Dynabeads Human T Activator CD3/CD28 beads (Thermo Fisher Scientific) at a ratio of 0.75 beads ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the Human Cytokine Magnetic 30-Plex Panel (Invitrogen, Cat No. LHC6003M) and plates were read using a Luminex200 machine with xPONENT software (v4.3) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed in PBS and incubated with anti-human Alexa-647 (1:500, ThermoFisher), and Hoechst 33342 (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were activated with Dynabeads Human T Activator CD3/CD28 beads (Thermo Fisher Scientific) at a ratio of 0.75 beads ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...