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Citations for Thermo Fisher :
4201 - 4250 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... and the concentration of the recombinant plasmids was determined using the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851). 293FT cells were transfected with 1 µg of recombinant plasmids or blank vectors with 40 ng of pRL-TK Renilla luciferase control vector using Lipofiter ...
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bioRxiv - Genomics 2023Quote: ... residual RNA contamination was removed by incubating the samples in 10µL of 1mg/mL recombinant RNase (Ambion, #AM2269). Sample aliquots of 100µL were kept at -80 °C.
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bioRxiv - Neuroscience 2023Quote: ... 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B], 3.33 mM each dNTP [ThermoFisher, R1121] ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Evolutionary Biology 2023Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The recombinant plasmids were transfected into 293T cells using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). For RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies and recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...
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bioRxiv - Immunology 2024Quote: Recombinant rabbit mAbs and Fabs were expressed in HEK293F cells using ExpiFectamine™ 293 Transfection reagent (Gibco, A14525). Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was in vitro transcribed from the linearized recombinant infectious clone using the mMESSAGE mMACHINE Kit (Ambion) and electroporated into Vero E6 cells ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000, Santa Clara, California and Human Brain Total RNA brRNA - ThermoFisher AM7962, Waltham, Massachusetts), as well as no-cell negative controls (1X TempO-Seq lysis buffer alone ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on the Clariom™ S Human Array to measure gene expression at >20,000 genes in the human genome (Affymetrix, Santa Clara, CA). Raw data generated from the arrays were read into R statistical software (version 3.5.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI–human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Biochemistry 2019Quote: ... Site-directed mutagenesis of plasmids to generate the C-terminal Myc tagged ECH1 catalytic mutants and the C-terminal V5 tagged UCP3 truncation mutants was carried out with Platinum pFX DNA polymerase from (Life Technologies, Grand Island, NY), following the manufacturer’s protocols for site-directed mutagenesis ...
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bioRxiv - Genomics 2022Quote: ... we performed end repair and then selectively purified biotin-tagged DNA using streptavidin coated beads (DYNAL™ MyOne™ Dynabeads™ Streptavidin C1, Invitrogen 65001). A-tailing and Illumina TruSeq adapter ligation (Illumina ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated cRNA libraries were synthesized from 250 ng of total RNA using the Affymetrix HT 3’ IVT Plus kit (Affymetrix, Santa Clara, CA) and the Beckman Biomek FXp Laboratory Automation Workstation (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Immunology 2021Quote: ... biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135; 10 kD BDA Invitrogen D1956; diluted in 0.9% NaCl) were used as retrograde and anterograde tracers ...
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bioRxiv - Genomics 2022Quote: ... The precleared nuclear lysates (2 mg) were incubated with purified biotinylated RNA in NT2 buffer along with Yeast tRNA (20 µg/ml; Thermo Fisher Scientific #AM7119) with gentle rotation for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... From each sample 250ng of total RNA were used to generate amplified and biotinylated sense-strand cDNA according to the GeneChip WT PLUS Reagent Kit User manual (Affymetrix, Santa Clara, CA). GeneChip Human Gene 2.0 ST Arrays were hybridized for 16h in 45°C incubator ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent anti-rat (Alexa 594) and biotinylated anti-rabbit coupled with streptavidin fluorescent conjugated antibody (Alexa 488) (1:1000, both Life Technologies, Carlsbad, USA).
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bioRxiv - Molecular Biology 2020Quote: ... affinity columns were prepared by immobilizing the biotinylated antibody (2.5 μg of anti-Apo CIII) with streptavidin magnetic beads (Dynabeads, Life Technologies, Grand Island, NY). Following sample preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated cRNA libraries were synthesized from 500 ng of total RNA using the Affymetrix HT 3’ IVT Express kit (Affymetrix, Santa Clara, CA) and ta Beckman Biomek FXp Laboratory Automation Workstation (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100ng of total RNA was used for biotinylated cDNA synthesis using GeneChip™ WT Plus Reagent Kit (Invitrogen, 902281, lot no. 01059768) as per manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... IgGs were randomly biotinylated at primary amines using EZ-link NHS-PEG4 Biotinylation Kit according to the manufacturer’s protocol (Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 RBD (2.5 µg/mL ...