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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Briefly, ligands (DinB, DinBΔPAD, and DinB-PAD) were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly dissolved in H2O ...
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bioRxiv - Physiology 2022Quote: ... Immunoreactivity of the anti-VGAT rabbit antibody was detected by incubation with biotinylated donkey antibody to rabbit IgG followed by incubation with streptavidin-conjugated Alexa647 (1:400; S21374, Thermo Fisher).
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bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in DMEM/F12 medium and incubated with biotinylated EGF conjugated with Alexa Fluor 647-streptavidin (EGF-647; 2 μg/ml; Life Technologies) for 30 to 40 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated with biotinylated secondary antibody and developed with HRP and substrate using a DAB substrate kit (Thermo Fisher Scientific). Primary and secondary antibodies used in this study are listed in Supplemental Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... were processed to generate amplified and biotinylated sense-strand cDNA targets using the GeneChip® WT PLUS Reagent kit from Affymetrix according to the manufacturer’s specifications ...
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bioRxiv - Genetics 2023Quote: ... Short fragments up to 1000 bp were produced via tagmentation and the biotinylated restriction junctions were then pulled down using MyOne C1 streptavidin beads (Life Technologies). PCR amplification (5 cycles ...
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bioRxiv - Microbiology 2023Quote: ... double-biotinylated DNA fragments (215 bp) were obtained by PCR amplification of custom-synthesized DNA fragments (StringsTM DNA Fragments; Invitrogen, Germany) with biotinylated primers (Bio-icsB-for/Bio-icsB-rev) ...
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bioRxiv - Plant Biology 2023Quote: Qdot-labeled Cel7A was prepared by mixing 3 nM biotinylated Cel7A with 2 nM Qdot 655 (Thermo Scientific; catalog number: Q10123MP) in 50 mM sodium acetate ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed using the PTFE filters and resuspended in 1x PBS or further biotinylated with 6 mg/ml EZ-link™ Sulfo-NHS-SS-biotin (#21331; ThermoFisher). Streptavidin conjugated to either AlexaFluor-488 or -568 was then added for 1h RT incubation ...
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bioRxiv - Immunology 2023Quote: 1-5 μg of total RNA from 2-3 MEF samples per group was used to prepare biotinylated RNA using the Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The protein concentration was determined and the antibodies biotinylated by incubating for 30 min at room temperature with EZ-Link NHS-PEG4-Biotinylation reagent (Thermo Scientific) at a 10:1 biotin-to-protein ratio ...
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bioRxiv - Biochemistry 2023Quote: ... and depleted the remaining biotinylated materials from the filtrate with 25 µL (slurry volume) of Pierce streptavidin magnetic beads (Thermo Scientific). Peptides in the supernatants were cleaned-up with C18 ZipTips (EMD Millipore ...
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bioRxiv - Immunology 2023Quote: ... Phages presenting GSDMD-specific VHHs were enriched using chemically biotinylated GSDMD immobilized on Dynabeads™ MyOne™ Streptavidin T1 (Life Technologies). The retained phages were used to infect E ...
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bioRxiv - Immunology 2023Quote: ... The nuclear extract was then spun at 21,000g for 5 minutes and the supernatant recovered and biotinylated using an EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Scientific). The solution was dialyzed using Slide-A-Lyzer® Dialysis Cassette G2 (cat# 87724 ...
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bioRxiv - Neuroscience 2023Quote: ... injections into the spinal cord rostral to the lesion as a retrograde tracer or injections of biotinylated dextran amines (BDA, D1956; 10,000 kD, 10.0% w/v, ThermoFisher Scientific, Waltham, MA) into the motor cortex as an anterograde tracer to visualize the CST ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific, 5082385) for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001) by rotating the tube for 15 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Transfected COS7 cells on a 100-mm dish were biotinylated by incubation in 1 mg/ml NHS-SS-Biotin (21331, Thermo Scientific), diluted in 1× PBS containing 1 mM MgCl2 and 0.1 mM CaCl2 (PBS–MC ...
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bioRxiv - Developmental Biology 2023Quote: ... they were incubated for 10 min at RT with biotinylated polyvalent secondary antibodies (UltraVision Detection System HRP kit, Thermo Fisher Scientific). After three 5-min washes in PBS ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Retinal flat mounts were prepared and stained with biotinylated Isolectin GS-IB4 from Griffonia simplicifolia (0.2 mg/ml; Invitrogen, Carlsbad, CA) and Texas red-conjugated avidin D overnight at 4°C [29 ...
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bioRxiv - Biophysics 2024Quote: ... MSP1E3D1 was expressed and purified as previously described (79) and biotinylated with EZ-Link NHS-polyethylene glycol 4 (PEG4)-Biotin (Thermo Fisher) per manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining portion of each sample was used in Streptavidin DynabeadTM-mediated pulldowns to isolate biotinylated RNA fragments following manufacturer protocol (ThermoFisher, 65001). Briefly ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells stably expressing a single copy of N-terminally tagged TTBK2- or GFP-BirA* were generated using Flp-IN T-REx cells (ThermoFisher K6500-01), Positive clones were selected with hygromycin resistance ...
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bioRxiv - Cell Biology 2020Quote: ... was used at a 1:1000 dilution overnight at 4°C followed by incubation with fluorescently tagged goat anti mouse-A647 and donkey anti-rabbit-A555 secondary antibodies at 1:100 dilution (Invitrogen, Carlsbad, CA). TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 WT cells and the UPF3 dKO clone 2 expressing either FLAG-tagged GST or UPF2 were labeled by culturing them for at least 5 passages in DMEM for SILAC medium (Thermo Fisher Scientific) supplemented with 9% FBS (Silantes) ...
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bioRxiv - Molecular Biology 2020Quote: HA-tagged ubiquitin (a gift of the F. Sicheri lab) was overexpressed in ES cells by transfection with Lipofectamine 2000 (Thermo Fisher Scientific), 500 ng per ∼8×106 cells in a 10 cm dish ...
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bioRxiv - Molecular Biology 2020Quote: ... the primary antibody was rinsed three times with PBS then Alexa Fluor 546-tagged goat anti-rabbit IgG secondary antibody (ThermoFisher, A-11035) was added at a 1:1250 dilution and incubated for 1 hour before a final 3x rinse with PBS.
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bioRxiv - Developmental Biology 2020Quote: ... The next day the antibody was washed five times in PBST 0.1% with gentle agitation at room temperature (30 minutes each) and fluorescently-tagged by horseradish peroxidase-catalysed signal amplification (Thermo Scientific, T20913)
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bioRxiv - Plant Biology 2020Quote: ... To create the 6xHis-tagged MBP fusion of full-length WUS the coding sequence was amplified with Phusion DNA Polymerase (Thermo Fisher Scientific), digested with NcoI/XhoI and ligated into pMG21053 previously digested with NcoI/SalI ...
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bioRxiv - Cell Biology 2020Quote: ... For transient overexpression (Fig 2 E-H, S2G) seipin tagged with C-terminal EGFP [34] was inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific) through HindIII/NotI sites ...
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bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by staining with the corresponding fluorescent tagged secondary antibodies for 1 hour at room temperature: goat anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and incubated in AlexaFluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific, catalog #A21039), AlexaFluor-647-tagged donkey anti-mouse IgG ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 2 μg of phCMV3 plasmids encoding the C-terminal HA-tagged NA from the strain of interest using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... E14.5 Apcdd1 mutant and wildtype control brains were cryosectioned at 16 microns and immunolabeled with Alexa488-tagged Isolectin (1:100; Thermo Fisher Scientific); Calbindin (1:1,1000 ...
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bioRxiv - Immunology 2020Quote: 293 ET cells were plated at 250,000 cells/well in six-well plates and a day later were transfected with 1 μg of pBent2-HA-IRF5 and 1 μg of plasmid encoding one of the myc-tagged candidate IRF5 kinases (in the pEAK8-myc vector) using Lipofectamine2000™ (Life technologies) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The amounts of peptides to be tagged were normalized and mixed with 41 μL of TMT reagent (TMT sixplex, Thermo Fisher Sci) freshly dissolved in acetonitrile (20 μg/μL ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, ThermoFisher, cat# A-31573) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells were transiently transfected with plasmids expressing FLAG-tagged version of BPLF1/BPLF1-C61A using the lipofectamine 2000 (Invitrogen, California, USA) or jetPEI® (Polyplus transfection ...
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bioRxiv - Genetics 2022Quote: ... FRAP was performed on 3T3-L1 preadipocytes transiently transfected with GFP-tagged CIDEC were incubated with 200 μM of oleic acid and stained with Bodipy 558/568 C12 fatty acid (Molecular Probes, D3835) for 15 hours ...
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bioRxiv - Biochemistry 2021Quote: ... 4 µl of resuspended MTG1-3xFLAG tagged mitoribosome sample was directly applied onto the grid mounted in the Vitrobot chamber (Thermo Fisher Scientific) and incubated for 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa Fluor 594-tagged goat anti-guinea pig IgG (1:500, Thermo Fisher, cat# A-21202, A-21206 and A-11076) for 1.0 – 1.5 hours at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and rabbit anti-D5R antibody and donkey anti-rabbit secondary antibody tagged with Alexa Fluor™ 488 (#R37118, ThermoFisher Scientific Waltham, MA). The tissues were counterstained with wheat germ agglutinin (WGA ...
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bioRxiv - Immunology 2021Quote: ... 0.02% Triton X-100 and 200 μM ATP for 10 min at 30°C or with purified hexahistidine-tagged TBK1 (catalog # PV3504; Thermo Fisher Scientific) in buffer B containing 50 mM Tris [pH 7.5] ...
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bioRxiv - Neuroscience 2022Quote: ... DNAJB4 or DNAJB6 constructs and 2) GFP-tagged polyQ or desmin constructs into C2C12 cells using Lipofectamine LTX and Plus reagent (Thermo Fisher Scientific). The rate of the cells with aggregate was observed three days post-transfection.
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bioRxiv - Plant Biology 2023Quote: ... constructs coding for mCherry tagged versions of the peptides were generated in pK7m34GW-rolD∷EGFP destination vector using Multisite Gateway Technology (Thermo Fisher Scientific). The entry clones contained the same promoter fragments in pDONRP4-P1R which were used for complementation experiments ...
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bioRxiv - Immunology 2023Quote: Removal of the hexa-glutamate tag was carried out via 3C protease-based cleavage (ACRO Biosystems) for 24 hours at 4 °C followed by subtraction of the GST-tagged 3C protease via glutathione agarose (Thermo Fisher Scientific) and gel filtration (Superdex 200 ...