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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Recombinant Human B4GALT4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: The recombinant abGFP4 nanobody against GFP with a C-terminal cysteine was covalently coupled to SulfoLink resin (Thermo Scientific) consisting of cross-linked agarose beads with iodoacetyl groups ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2023Quote: ... Ganglioside M1 (GM1) staining was performed using recombinant Choleratoxin B labelled with AlexaFluor647 (3 µg/ml, Thermo Fisher, #C34778). Both GFP-D4 and AlexaFluor647-labelled CTB were added to FACS buffer-washed cells and incubated for 20 minutes at 4°C in the dark ...
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bioRxiv - Plant Biology 2023Quote: ... Preparation of recombinant bacmid DNA and transfection of Spodoptera frugiperda 9 (Sf9) cells were performed according to the manufacturer’s instructions (Invitrogen). SbCYP711A31 was expressed in Sf9 cells and microsomal fractions of the insect cells expressing SbCYP711A31 were obtained as described previously58 ...
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bioRxiv - Biochemistry 2022Quote: ... The pKL plasmid was used for the generation of recombinant Baculoviruses using the Bac-to-Bac expression system (Invitrogen).
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bioRxiv - Biophysics 2023Quote: ... Recombinant bacmids were generated from the plasmids in Table S1 using MAX Efficiency DH10Bac competent cells (Gibco; Cat. 10361012) and transfected into Sf9 insect cells (Gibco ...
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bioRxiv - Neuroscience 2023Quote: Four sections from each mouse were stained using primary antibodies calbindin D28K anti-rabbit recombinant polyclonal (1:200; ThermoFisher) and parvalbumin anti-mouse monoclonal (1:200 ...
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bioRxiv - Immunology 2023Quote: The DNA coding sequences of tagged recombinant GITRL and OX40L were produced by GeneArt Gene Synthesis service (ThermoFisher Scientific). The sequences of GITRL and OX40L constructs contain ...
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bioRxiv - Microbiology 2024Quote: ... the sequence of the resulting recombinant plasmid was ascertained by Sanger sequencing techniques (Applied Biosystems™ 3130 Genetic Analyser), and the resulting data were decoded by ApE software (v2.0.36 ...
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bioRxiv - Microbiology 2020Quote: ... microbial protein fermentation was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... microbial protein fermentation was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the supernatant for all protein samples was determined by Pierce BCA Protein Assay Kit (#23227, Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The protein concentration was determined using a BCA protein assay kit (Pierce® BCA Protein Assay Kit; 23225, Thermo Fisher Scientific Inc., Victoria, Australia). Gel electrophoresis was performed using Bolt 4–12% Bis-Tris Plus gels (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Protein purification was monitored using 12% SDS-PAGE and protein concentration was determined with the Qubit™ protein assay (Thermo Fisher Scientific, catalog no.Q33211).
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bioRxiv - Microbiology 2020Quote: ... The total protein content was measured using the Pierce BCA Protein Assay kit (Thermo Fisher) and 7.5 µg of lysate was run on a 4-12% Bis-Tris sodium dodecyl sulfate (SDS)-polyacrylamide gel with MOPS running buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using BCA protein assay kit (ThermoFisher Scientific; Waltham, MA, United States) in accordance with manufacturer’s instructions and 10 μg of protein was loaded per lane (N = 3 - 4) ...
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bioRxiv - Cancer Biology 2021Quote: One mg protein sample was precleared with Dynabeads Protein A (Thermo Fisher, UK, Cat.100002D) and 1 μg isotype IgG2a (Cell Signalling ...
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bioRxiv - Cancer Biology 2021Quote: ... p53-associated protein were pulled down by 5 μl DynabeadsTM protein G beads (#10004D, Invitrogen) at 4 °C for 1-1.5 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined by a Pierce BCA protein assay (Life Technologies, #23225). Equal amounts of protein were loaded into 8% Bolt™ Bis-Tris Plus gels (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was performed using a Pierce Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) and 300 μg of proteins were used for O-glycans enrichment adapted as previously described (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein levels were normalised across samples using a Pierce™ BCA protein assay kit (ThermoFisher) according to manufacturers instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was collected for protein concentration quantification with BCA Protein Assay Kit (ThermoFisher Scientific). An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were separated with the NuPAGE system (ThermoFisher, 4-12% Bis-Tris protein gel), and transferred to PVDF membrane (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... and the protein concentration was determined using the BCA Protein Assay Kit (#23227, Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration was measured using a Pierce BCA protein assay kit (Thermo Scientific Fisher). After boiling the samples in Laemmli buffer at 95°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined with the Pierce 660nm Protein Assay (Thermo Fisher Scientific, cat# 2262). Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were loaded in NuPAGE bis-tris protein gels (Thermo Fisher Scientific) and electroblotted to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration was assessed using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). The mitochondria were pelleted by centrifugation (14,000 x g ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
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bioRxiv - Microbiology 2019Quote: ... The proteins concentration was determined using a BCA Protein Assay Reagent Kit (Thermo Fisher Scientific) and stored at −80°C.
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bioRxiv - Genomics 2020Quote: ... 5 µg antibody was coupled to 50 µl Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was quantified using the BCA Protein Assay Kit (Fisher Scientific, Cat# 23227). Samples were normalized to a fixed amount of protein ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... and 0.01% bromophenol blue) and protein concentrations were measured using Pierce 660nm Protein Assay (ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations were measured using Pierce ™ BCA Protein Assay Kit (23225; Thermo Scientific) with a Albumin BSA Standards ranging from 2000 μg/mL to 0 μg/mL using a Nanodrop Spectrophotometer cuvette system (ND-2000C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific, Rockford, IL, USA). 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... then protein concentration was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). 300 μg lysates were pre-cleared with 2 μl Protein A Dynabeads (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein-protein complexes were eluted in NuPAGE™ LDS Sample Buffer (4x) (Thermo Fisher Scientific) at 100 °C for 10 min.
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was quantified using the Pierce ™ BCA Protein Assay kit (Thermo Fisher Scientific). 30 µg of total protein was loaded onto a 10% polyacrylamide gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... the protein quantity was measured using the Micro BCA protein assay kit (Thermo Fisher Scientific) and a SpectraMax microplate reader.
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of cell lysate was measured using the Coomassie protein assay kit (Thermo Scientific). Proteins were separated on SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... and protein levels in the postnuclear supernatants measured with Pierce BCA Protein Assay Kit (Thermofisher). After equalizing protein amount and concentration in all samples ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations for each fraction were determined by the BCA protein assay kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and measured the protein concentration using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). For comparing DAgs of different deltaviruses ...
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bioRxiv - Cancer Biology 2019Quote: ... The cultured cells were treated to extract proteins using a protein extraction kit (Thermo Fisher), with bovine serum albumin as the standard ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of protein (4 μg ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentrations were obtained for each homogenate using a BCA protein assay kit (Thermo Scientific) and samples were stored at −80°C until used for western blot analysis.