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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Cancer Biology 2021Quote: NUCKS1’s GRP DNA-binding motif was mutated to AAA using the Phusion Site-Directed Mutagenesis Kit (Thermofisher), with primers as follows ...
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bioRxiv - Cell Biology 2020Quote: ... The binding of the primary antibodies was quantitated with anti-mouse (1:2000, Life Technologies, Carlsbad, CA, USA) or anti-rabbit (1:3000 ...
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bioRxiv - Developmental Biology 2020Quote: ... the binding reactions were then transferred onto Biodyne B Nylon membranes (Thermo Fisher Scientific, Waltham, MA, USA, #77016) using 0.5X TBE for 1 h at 60 V ...
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bioRxiv - Bioengineering 2019Quote: Open biotin binding sites on the carriers after STP coating was quantified indirectly using FITC-biotin (Thermo Fisher). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... female worms by holding the specimen vertically above a 2 ml low binding microcentrifuge tube (Fisher Scientific, UK) and carefully snipping <3 mm tissue from the tail (posterior to the anal pore and the major ganglia ...
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bioRxiv - Bioengineering 2020Quote: ... media was removed and replaced with 30 µL 5 nM PLA probes (2.5 nM probe A and 2.5 nM probe B) in Probe Binding Buffer (PBS, 0.1% BSA (Thermo Scientific), 0.1 mg/ml sonicated salmon sperm DNA (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: WT and mutant R248W p53 DNA binding domains (DBDs) were cloned using the Gateway system (Thermo Fisher Scientific). Genes for WT and R248W p53 DBDs were respectively amplified from the vector pCMV-Neo-Bam carrying WT and R248W p53 constructs ...
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bioRxiv - Microbiology 2020Quote: ... Binding of primary antibodies were visualized using Alexa 488 α-rabbit and 568 α-mouse secondary antibodies (Invitrogen). The S and HN proteins expression were analysed through fluorescence for wild-type and recombinant viruses compared to mock infected cells ...
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bioRxiv - Microbiology 2021Quote: ... An anti-ICAM-1 monoclonal antibody (15.2) that blocks the PfEMP1 binding site (Thermofisher, MA180910, 10 μl/ml) was used as blocking antibody as described (28) ...
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bioRxiv - Microbiology 2020Quote: ... Selected plasmids with binding-site fragments were sequenced and then quantified using a NanoDrop 2000c spectrophotometer (Thermo Scientific). The mutational sequence of each binding site was synthesized by BGI-ShenZhen and cloned into the psicheck-2 vector using the method described above ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of S1 to ACE2 transfected cells was measured with an Alexa Fluor-647-conjugated streptavidin (Invitrogen, S21374) diluted 1:500 using a CytoFLEX flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... The binding of anti-GFP-HRP was revealed by the addition of 4.5 mL peroxide substrate (Pierce Thermofisher) + 0.5mL chromogen DAB (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Genomics 2019Quote: ... containing 960 µL of lysis/binding solution plus 80 µL of the Plant RNA Isolation Aid (Ambion, USA) on a FastPrep®-24 Instrument (MP Biomedicals ...
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bioRxiv - Immunology 2019Quote: ... and resuspended in the appropriate volume of Lysis/Binding Buffer from the Dynabeads mRNA DIRECT Micro Kit (ThermoFisher). mRNA were isolated directly with dynabeads oligo (dT ...
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bioRxiv - Microbiology 2019Quote: ... pneumococci cells were labelled with cell permeant SYTO13 green fluorescent DNA binding dye (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instruction prior to infecting J774A.1 cells ...
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bioRxiv - Genomics 2019Quote: ... containing 960 µL of lysis/binding solution plus 80 µL of the Plant RNA Isolation Aid (Ambion, USA) on a FastPrep®-24 Instrument (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: ... Hb binding partners were identified by ESI-LC-MS/MS mass spectrometry (LTQ-Orbitrap Velos, Thermo Fisher Scientific) using Proteome Discoverer 1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Assays for concentration-dependent RBD binding (Fig. 1E) were developed using 1-step Slow TMB (Thermo Fisher Scientific). EC50 was determined from non-linear regression models using Graph Pad Prism 8.
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bioRxiv - Genomics 2021Quote: ... followed by adding 100 μl of Lysis/Binding Buffer in Dynabeads® mRNA DIRECT™ Micro Kit (Invitrogen) and pipetting up and down to lyse the cells ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed once in PBS and then washed once in 1X Annexin-V Binding Buffer (ABB) (Invitrogen). The cells were resuspended at a density of 1 x 106 cells/ml in 1X ABB ...
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... an LR reaction was used to shuttle these multimerized binding sites into the pGREEN binary described above (Invitrogen). Cloning primer sequences for all constructs are listed in Table S7 ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 min and non-specific binding sites were blocked with Ultra-V Block solution (Thermo Fisher Scientific) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... pH 7.4 and added (100μL/well) to 96-well microtiter plates with high binding capacity (Thermo Fisher, USA) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... were added to the binding reactions followed by 1.2 µL of diluted DNAse I (0.625 U total, Invitrogen) and reactions were incubated at room temperature for 5 minutes ...
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bioRxiv - Genomics 2024Quote: A vector containing the Streptavidin-Binding Peptide (SBP)-Tag was prepared in pEF1 (Thermo Fisher, Cat# V 92120), and SERBP1 ORF was cloned in frame with the His-Myc-tag present in the vector to create pSBP-SERBP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and nascent RNAs were isolated by Streptavidin bead binding (Dynabeads® MyOneTM Streptavidin T1 magnetic beads, ThermoFisher Scientific). Following 3’ adapter ligation ...
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bioRxiv - Microbiology 2023Quote: ... The water samples were stained with the DNA-binding fluorescent dye SYBR green I (Invitrogen, final concentration 2x), vortexed and incubated for 15 minutes in the dark at 37° C ...
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bioRxiv - Biophysics 2023Quote: ... Blots were rinsed in distilled water and antibody binding was detected with chemiluminescent solution (Pierce, Fisher Scientific, France) and visualized under a ChemiDoc MP imaging system (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... PGCLCs were aggregated with isolated gonadal somatic cells in a low-binding 96-well plate (174929, Thermo Scientific) for 2 days in GK15 medium supplemented with 1 μM retinoic acid ...
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bioRxiv - Microbiology 2023Quote: ... Soluble EFNB2-Fc was constructed by cloning the receptor binding domain (a.a. I28 to G165) of EFNB2 into the NheI-BamHI sites of pcDNA3.1(+) (Invitrogen) with a N-terminal CD5 leader (MPMGSLQPLATLYLLGMLVASVLA ...
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bioRxiv - Biochemistry 2023Quote: ... and the binding of each clone to SN-38 were analyzed using the Attune flow cytometer (Thermo Fisher). The sequences with the best binding signals were tested for binding using BLI.
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAC1 was detected using PAK1-p21-binding domain (PBD) pull-down and Detection Kit (Thermo Scientific) as instructed by the manufacturers.