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Citations for Thermo Fisher :
4101 - 4150 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Acquired spectral data files were searched against the human component of UniProtKB/SwissProt database using SEQUEST search engine in the Proteome Discoverer 1.4 (Thermo Scientific). The following parameters were applied ...
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bioRxiv - Cancer Biology 2023Quote: ... at different timepoints as the percentage of hCD20pos cells (anti-human CD20 FITC, clone 2H7) compared to murine CD45pos cells (anti-mouse CD45 PE, clone 30F11, ThermoFisher). When indicated ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Microbiology 2023Quote: ... a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (1:500 dilution; #43700, Invitrogen) and a peroxidase-conjugated goat anti-mouse secondary antibody (1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Human intestinal epithelial cells (HIEC-6) were cultured in Opti-MEM™ I Reduced Serum Medium (Thermo Scientific, MA, USA). Cell viability was measured using MTT assays (ref) ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney (HEK293) cells (RIKEN Cell Bank, Japan) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO, 11965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... A 10% (w/v) solution of biomaterial was prepared by manually mixing the pulverized biomaterial in Human endothelial serum free media (HESFM; Gibco) supplemented with fetal bovine serum (2% v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Developmental Biology 2023Quote: A small piece of primary human or mouse tissue was resected and embedded in Fisher Healthcare Tissue-Plus Optimum Cutting Temperature (OCT, ThermoFisher). This was frozen at -80 0C ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Cancer Biology 2023Quote: The 387 complex genetics sarcomas were analyzed on Human Genome U133 Plus 2.0 array (900466, Affymetrix, Santa Clara, CA, USA), according to the manufacturer’s procedures ...
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bioRxiv - Microbiology 2022Quote: ... PBMCs were isolated by density gradient using Lymphoprep (Axis-Shield) and CD4+ T cells purified by negative selection using the Dynabeads Untouched Human CD4+ T Cell Isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: 1000-3000 IEQ human islets pellets were resuspended in 200 µL PBS and treated with 20 µL 10 mg/mL Rnase A (Invitrogen) and 20 µL Protein Kinase K (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... cells were harvested and stained for cell surface CD4 expression using anti-human CD4 APC (RPA-T4, eBioscience, ThermoFisher Scientific) and analysed by flow cytometry on a BD FACSCanto II system (BD Biosciences ...
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bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: RT-qPCR with TaqMan primer/probes was used to determine relative expression levels of miR-146a compared to U18 (human) or sno251 (mouse) endogenous control genes (ThermoFisher, assay IDs ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: ... and maintained in Ultiβ1 cell culture medium (Human Cell Design) containing 100 U/mL penicillin and 100 μg/mL streptomycin (Life Technologies). Based on the experimental setup ...
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bioRxiv - Microbiology 2023Quote: ... β-actin expression was measured using an anti-human β-actin horseradish peroxidase-conjugated mAb (1:25000, Life Technologies, USA) as the loading control ...
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bioRxiv - Microbiology 2023Quote: Type I RH parasites (ATCC PRA-319) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) and maintained in DMEM (GIBCO) supplemented with 2 mM glutamine ...
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bioRxiv - Microbiology 2023Quote: ... CVCL_1926) and HEK293T engineered to overexpress human ACE2 (BEI NR-52511, RRID: CVCL_A7UK) were maintained in DMEM (Gibco, 11965-092) supplemented with 10% FBS (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human endogenous PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... human pluripotent stem cells were dissociated using Accutase and plated at high density on Geltrex (#A14133-02, Thermo Fisher Scientific) coated wells ...
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bioRxiv - Cancer Biology 2023Quote: ... Viably frozen primary AML cells were thawed and CD3-depleted with biotinylated anti-human CD3 (SK7) and biotin-binder Dynabeads (Invitrogen) and subsequently injected via the lateral tail vein into 2.8 Gy irradiated (24h before transplant ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells or MiniBAR-GFP stable cell lines were transfected with 25 nM of siRNAs targeting either luciferase or human Mini-BAR (5’-CUGCAAAUUUUACGGAUCA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Human TSC media (TSCm) used herein was previously described in 70 with slight modifications: 470 ml DMEM/F12 (Invitrogen 21331), 5 ml Commercial N2 supplement (Invitrogen 17502048) ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T) cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... TJC4) was detected using a 1:500 dilution of goat anti-human Alexa Fluor 488 or Alexa Fluor 647 (Invitrogen). Secondary antibodies were incubated for 15 min at 4°C in the dark ...
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bioRxiv - Biophysics 2023Quote: ... Clathrin Heavy Chain siRNA (Human CLTC, sequence: GGUUGCUCUUGUUACG, ID: s475) and Negative Control#1 siRNA Silencer Select were purchased from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2023Quote: ... of the target genes together with a human GAPDH endogenous control and the use of 7500 SDS software (Applied Biosystems). The primers used in this study were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... The pENTR-hMCAK construct was generated by amplifying the coding sequence of human MCAK by PCR and inserting it into a pENTR entry clone using the pENTRTM/D-TOPO kit (ThermoFisher). The pFB-6HF2hMCAK construct was created from pFB-6HFRET2-DEST vector and pENTR-hMCAK constructs using Gateway® cloning technology (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Bioengineering 2023Quote: Human mammary gland epithelial cell adenocarcinoma cell line MDA-MB-231 was maintained in F-12K (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...
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bioRxiv - Bioengineering 2023Quote: Human mammary gland epithelial cell adenocarcinoma cell line MDA-MB-231 was maintained in F-12K (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Genetics 2023Quote: ... was extracted from skin fibroblasts derived from two different Welsh Mountain ponies that were overexpressing human SOX11 (as described above) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labelled with phycoerythrin (PE)-conjugated anti-human-HLA-ABC (clone W6/32, Invitrogen 12-9983-42; 1:20), biotin-conjugated anti-mouse-H-2Kb (clone AF6-88.5 ...
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bioRxiv - Microbiology 2023Quote: ... Human alveolar A549 cells were seeded in an 8 well chamber slide (Lab-Tek II Chamber Slide w/ Cover, NUNC), and once confluent ...
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bioRxiv - Immunology 2023Quote: ... purified Treg cells (5 × 104) were cultured with Dynabeads Human T-activator CD3/CD28 (12.5 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Immunology 2023Quote: ... The low and high molecular weight membranes proteins were incubated with respective primary antibodies (Rabbit anti-human BAIAP2L1, PA5-54000, Invitrogen) or (Goat anti-mouse GAPDH ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed 5x and incubated with 100 µl/well with an HRP-conjugated anti-human IgG Fc antibody (1:10000 dilution, Invitrogen) for 1 hour ...
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bioRxiv - Genomics 2023Quote: The human lung fibroblast cell line IMR-90 was purchased from ATCC and cultured in EMEM (ATCC) with 10% FBS (Gibco) and 1% penicillin/streptomycin ...