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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined using a BCA protein kit assay (Thermo Scientific), and 10µg of protein was used per lane for western blotting ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein content was quantified using BCA Protein Assay (Thermo Fisher Scientific) and 40 ng of protein were loaded as previously described (55) ...
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bioRxiv - Synthetic Biology 2024Quote: ... along with protein ladders (SeeBlue TM Plus2 Pre-stained Protein Standard, Invitrogen). Samples were run at 150 kV ...
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bioRxiv - Plant Biology 2024Quote: ... Protein estimation was conducted with Pierce BCA Protein Assay kit (Thermo Scientific). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... and protein levels were quantified using the Pierce 660 Protein Assay (ThermoFisher). Quantified lysates were normalised to 0.5-1.5mg/ml ...
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bioRxiv - Pathology 2024Quote: ... Proteins were quantified in EV lysates using Qubit Protein Assay kit (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: Protein samples were loaded to the protein gel (Thermo Fisher Scientific, #NP0323BOX) at ∼10– 13 μg per lane ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein lysates were quantified using the Bradford protein assay (Thermo Fisher Scientific), and 20 μg were electrophoretically separated on 12% Tris-Glycine Gels (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... SUMOylated-Menin proteins were immunoprecipitated with Dynabeads™ Protein A beads (Invitrogen) coupled to Xpress tags or Protein A/G-Agarose (GenDEPOT ...
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bioRxiv - Physiology 2024Quote: ... Protein was measured using the BCA Protein Assay kit (Thermo Scientific™), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were determined using Pierce™ BCA Protein Assay (ThermoFisher 23225). Laemmli sample buffer (BioRad 1610747 ...
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bioRxiv - Microbiology 2024Quote: ... Protein was quantified via Pierce BCA Protein Assay Kit (ThermoFisher Scientific, 23225). 15μg-30ug of protein was loaded per sample into a 10% polyacrylamide gel and ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentrations were determined by bicinchoninic acid protein assay (Thermo Fisher Scientific) and 200 μg of each biological replicate prepared for digestion using Micro S-traps (Protifi ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal protein amounts of cell lysates or equal volumes of cell supernatants were prepared in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific, Boston, MA, USA), containing reducing agent (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant reporter proteins were expressed as a soluble secreted proteins by transient transfection of suspension culture HEK293F cells (FreeStyle™ 293-F cells, Thermo Fisher Scientific, Waltham MA) for wild type glycosylated structures and in HEK293S (GnTI- ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotech) or the AttuneNxT acoustic focusing cytometer (4 lasers, 14 parameters, Thermo-Fisher Scientific). Data were processed using FlowJo software (ver.9.9 or 10.7 ...
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bioRxiv - Developmental Biology 2021Quote: ... Media was changed daily and the cells passaged every 3–4 days at a ratio of 1:4 using the StemPro EZPassage tool (ThermoFisher Scientific).
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bioRxiv - Genomics 2020Quote: ... The RNP was combined with 100 pmol of ssODN donor and delivered to cells by Neon electroporation (1500 V, 10 ms, 3 pulses; Neon Transfection System, Model MPK5000, Life Technologies). Single cells were screened for the expected mutations through sequencing paired-end on an Illumina MiSeq instrument (250 cycles) ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 ml of logarithmically growing yeast cells in DOA media were added to a 35 mm FluoroDish (Fisher Scientific; 15199112) which had been pre-incubated at 30°C with Concanavalin A (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Freshly isolated CD4+ T cells were activated for 3 days on CD3/CD28 activation beads (Dynabeads ThermoFisher Scientific, Loughborough, UK; #11132D) as before ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were dehydrated with ethanol series (70%, 95%, then 100%) and hybridized with TAMRA-OO-(TTAGGG)3 PNA probe (Applied Biosystems) in formamide hybridization solution (70% formamide ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in a ratio of 3:1 and total RNA was extracted with Ribopure Yeast Kit (Ambion, Life Technologies) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2019Quote: ... 1.0 x105 cells were seeded in 12-well plates (n=3/condition/cell-type) and cultured in monolayer in differentiation media (StemPro Osteogenic Media; ThermoFisher Scientific) for 28 days ...
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from pooled EGFP FACS-sorted cells from the guts of 3 smMHC/Cre/EGFP embryos at E10.5 using TRIzol (Life Technologies, USA) and cleaned on RNeasy columns (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Cells were washed 3 times with PBS and resuspended in RPMI 10% FBS containing 5ug/mL Acridine Orange (A3568, Invitrogen, Switzerland) to a concentration of 0.2 x 106 cells per mL ...
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bioRxiv - Cancer Biology 2020Quote: ... and invasive cells attached to the bottom membrane of the insert were stained with Hema 3(tm) Fixative and Solutions (Fisher Scientific) after 24-hour incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... After 3 washes with phosphate-buffered saline (PBS), cells were incubated with TMB (3,3’, 5,5’-tetramethylbenzidine)-stabilized chromogen (Thermo Fisher, Cat# SB02) for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and transfected into GFI1-3×FLAG CCRF-CEM cells by electroporation using the Neon transfection system 10 μL kit (ThermoFisher Scientific). Electroporation parameters were 1500V ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were passaged every three to five days after a 3 min incubation with 0.5 mM EDTA (Thermo Fisher Scientific). For the cell growth assays ...
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bioRxiv - Immunology 2020Quote: ... Cells were then mixed with 40 μL of phenotype panel containing surface marker antibodies (Supplementary Table 3) and a dead cell marker (LIVE/DEAD Fixable Near-IR; Invitrogen L10119) (final dilution 1/1000) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then washed 3× with PBS and incubated for 1h with the corresponding Alexa Fluor secondary antibodies (Thermo Fisher Scientific) at 1:1000 dilution (in 3% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: The following steps were conducted in 0.2 mL tubes (Axygen Biosciences): 3 μL of D2 buffer from a REPLI-g Single Cell Kit (Thermo Fisher Scientific) was added to 4 μL of droplet suspension ...
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bioRxiv - Immunology 2022Quote: ... and H1 SI06 (A/Solomon Islands/3/2006) were produced by transient transfection of the corresponding plasmid(s) in Expi293 cells (Life Technologies) using Expifectamine293 transfection kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Five million C4-2 or two million SK-OV-3 cells in serum free media were mixed one to one with growth factor reduced Matrigel® (Invitrogen) in a total volume of 200 μl and injected in the hind flank using a 23- or 27-gauge needle ...
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bioRxiv - Microbiology 2020Quote: ... cells were infected or mock infected with VSV at a MOI of 3 in serum/phosphate-free DMEM (Gibco #11971-025). 10 μg ml−1 Actinomycin D (Sigma #A5156 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then resuspended in DMEM + 10% FBS supplemented with 50 μM CellEvent™ Caspase 3/7 Green Detection Reagent (Invitrogen) at 37 °C for 25 min ...
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bioRxiv - Cell Biology 2021Quote: ... The pooled cell suspension was centrifuged for 3 minutes at 1000 rpm in Heraeus Megafuge 16R Centrifuge (Thermo Fisher Scientific, USA), the supernatant removed and the remaining cell pellet re-suspended in epithelial medium containing 3:1 mixture of low-glucose DMEM:F12 supplemented with fetal calf serum 10% ...
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bioRxiv - Cancer Biology 2022Quote: ... Migrating cells on the lower side of the membrane were fixed and stained with the Hema 3 Staining kit (Thermo Scientific). Migrating cells in each well were counted in five random fields by contrast microscopy using an Eclipse E200 Nikon microscopy (4X magnification ...
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bioRxiv - Bioengineering 2022Quote: ... the HE_P0 cells were dissociated and split at a ratio of 1:3 in a defined medium composed of DMEM-F12 (Gibco, 11320033), 1× ITS (Merck ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... Cells were then washed with PBS (3 × 5 min) before incubation with anti-rabbit AlexaFluor 647 secondary antibody (Life Technologies A21245) at 1:500 dilution in antibody dilution buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Pathology 2020Quote: ... Whole lanes containing TbGPI3 and wild type cell lines samples were cut identically into 3 slices and the gel pieces were dried in Speed-vac (Thermo Scientific) for in-gel reduction with 0.01 M dithiothreitol and alkylation with 0.05 M iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were washed 3 times in 1 ml TET and imaged on a fluorescence microscope (EVOS cell imaging systems, Thermo Fisher) at constant light intensity ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was then biotinylated by incubating cells for 3 min with 30 mg of EZ-Link-Sulfo-NHS-LC-Biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed 3 times in PBS for 5 minutes before being incubated with AlexaFluor 488 or AlexaFluor 568 (Thermo Fisher) rabbit or mouse secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... EmbryoMax was removed and cells were added at 3 x 104 cells per well and allowed to reach 70-80% confluence before fixation in 3.7% formaldehyde (Fisher Scientific #BP531) in PBS for 10 minutes at room temperature ...