Labshake search
Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... cultures were treated with 2.5 µM MPP+ at DIV11 and THGFP-positive cells were collected from coverslips using a glass pipette at DIV 14 and placed directly in an aliquot containing 0.5 ml of RNase Out (Thermo Fisher Scientific, Waltham, MA, USA) and 0,5 ml of DTT (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2022Quote: ... each well of a 96-well plate containing 1-2 individual organoids or media alone were seeded with 5000 cells per well and cultured for up to 14 days in StemPro (Thermo Fisher Scientific, Cat#10639011) supplemented with Phase IV cytokines ...
-
bioRxiv - Plant Biology 2021Quote: ... The cells were then washed in PBS and placed individually on the cap of an 8-tube PCR strip (Axygen Low Profile 8-Strip PCR Tubes; Fisher Scientific 14-223-505). The presence of a single meiotic cell without attached debris was confirmed microscopically during both pick-up and release (10X magnification ...
-
bioRxiv - Cancer Biology 2019Quote: ... Braunschweig, Germany), and MOLM-14-TKD (provided by Dr. J Tamburini, Paris) were cultured in RPMI 1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA), supplemented with 10% Fetal Bovine Serum (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: Single hepatocytes or bulk stroma were laser-capture microdissected and DNA was extracted by incubating the tissue overnight at 65 °C in 14 μL of Arcturus® PicoPure® DNA Extraction buffer (ThermoFisher, Cat # KIT0103). Lysates were then incubated at 95 °C for 10 min to inactivate Proteinase K activity ...
-
bioRxiv - Physiology 2020Quote: ... A 14 mm section of spinal cord surrounding the lesion site was dissected and frozen in Shandon M1 embedding matrix (Thermo Fisher Scientific, Waltham, MA). 25 μm coronal sections of cord were collected using a freezing microtome and every 20th slice was mounted onto charged slides (Thermo Fisher Scientific premium frosted microscope slides ...
-
bioRxiv - Neuroscience 2021Quote: ... The cortices were homogenized without thawing in 5 times volume-weight of RIPA with a mechanical homogenizer (14-261-01, Fisher Scientific, Waltham, MA, USA). The hippocampi were homogenized without thawing by sonication in 150µL of RIPA ...
-
bioRxiv - Bioengineering 2021Quote: ... The cultures were allowed to grow for a total of 48 hours with 100uL of sample taken every 24 hours in order to measure the OD600 of the culture using a spectrophotometer (Fisher Scientific,#14-385-445).
-
bioRxiv - Developmental Biology 2022Quote: ... 25 mM HEPES in PBS) filtered through a 100 μM cell strainer and resuspended in FACS buffer with Fixable Viability dye (Invitrogen, Cat #65-0863-14) at a dilution of 1:1600 ...
-
bioRxiv - Neuroscience 2022Quote: ... or the whole bodies of 2-day-old (for PSMD9 and RpL32) or 14-day-old (for HSPs and RpL32) flies by using TRIzol (Thermo Fisher Scientific Cat# 15596018). cDNA was generated with a PrimeScript RT reagent kit (Takara Cat# RR047A) ...
-
bioRxiv - Pathology 2024Quote: ... This created a tet-on expression system in which TDP-43ΔNLS was expressed under the ACTA1 skeletal muscle promoter only when mice received a doxycycline (dox) chow diet (200 mg/kg, Fisher Scientific, 14-727-450). As controls ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... at which time both animal weight and tumor size were measured thrice weekly using balance and a digital caliper (cat 14-648-17, Fisher Scientific, Federal Way, WA). Tumor volume was estimated using caliper measurements ...
-
bioRxiv - Immunology 2023Quote: Cells were stained for viability (Supplementary table 1 and Supplementary table 4) and blocked for CD16/32 with anti-human FC Receptor binding inhibitor (ThermoFisher Scientific, 14-9161-73) in PBS for 15 minutes at room temperature (Spn ...
-
bioRxiv - Immunology 2023Quote: ... For removal of erythrocytes from blood samples, heparinized mouse whole blood [Heparin Sodium (MOCHIDA, 224122458)] in the borosilicate glass tube (Fisher Scientific, 14-961-30) was mixed with an equal volume of 2% dextran solution [2% Dextran T500 (Pharmacosmos ...
-
bioRxiv - Developmental Biology 2023Quote: ... Viable cells were determined with live/dead cell marker (Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 455UV, ThermoFisher Scientific #65-0868-14). The following fluorescent conjugated antibodies at a 1:50 dilution were used:
-
bioRxiv - Cell Biology 2023Quote: ... and cell pellets were washed and then resuspended in 500 μl polysome buffer in the glass tube (Fisher Scientific, Cat. No.14-961-32). Afterwards ...
-
bioRxiv - Cancer Biology 2022Quote: ... at which time both animal weight and tumor size were measured thrice weekly using balance and a digital caliper (cat 14-648-17, Fisher Scientific, Federal Way, WA). Tumors were allowed to grow until they reached the humane limit of 1500- 2000 mm3 at which time animals were sacrificed and tumors were collected ...
-
bioRxiv - Biochemistry 2023Quote: ... Restriction-digested shuttle vectors -1-6 (33) and -2-1 (14) for wc-1 and wc-2 respectively were recombined with PCR (Thermo Fisher Scientific, Catalog # F549S) products amplified with primers bearing point mutations of wc-1 or wc-2 ...
-
bioRxiv - Immunology 2023Quote: Two million cells of each sample for each flow panel were stained as follows: Live/Dead staining (Fixable Viability Dye eFluor506, Thermofisher. Cat. 65-0866-14) for 10min at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... cells resuspended in PBS were stained using eBioscience fixable viability dye coupled with either Zombie Red (for LP) or eFluor 506 (for BMDM) (Invitrogen Cat# 65-0866-14) for 20 mins on ice ...
-
bioRxiv - Developmental Biology 2024Quote: In vitro differentiation to neural progenitor cells [14] was performed in N2B27 media consisting of a 1:1 ratio of Advanced DMEM/F-12 (Gibco, Cat. No. 21331-020) and Neurobasal medium (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... and then 5 ml of this reaction was added to 14 ml H2O and 1 µL of Proteinase K (Thermo Fisher, 20 mg/mL) at 50°C for 30 min ...
-
bioRxiv - Microbiology 2019Quote: ... Eluted proteins were analyzed by SDS-PAGE followed by Coomassie-staining or western blot using an anti-His primary antibody (Thermo Fisher Scientific, Waltham, MA, USA), HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Immunology 2020Quote: ... and human antibody (bound to phage) was immunoprecipitated using 40ul of a 1:1 mix of protein A/G magnetic beads (Thermo Fisher, Waltham, MA, #10008D & #10009D). Beads were washed 4 times and antibody-bound phage were eluted into 1ml of E ...
-
bioRxiv - Cancer Biology 2022Quote: ... Pierce™ ECL Western Blotting Substrate was used to detect protein-antibody complexes prior to visualization on CL-X Posure™ films (both from ThermoFisher Scientific, Waltham, MA, USA). The resulting dots were analyzed using ImageJ software (National Institutes of Health ...
-
bioRxiv - Physiology 2022Quote: ... Equal loading of protein samples from each group was evaluated using β-actin or GAPDH antibody after using Restore WB stripping buffer (Thermo Fisher Scientific, catalog 21059).
-
bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
-
bioRxiv - Immunology 2024Quote: ... Proteins were transferred from the gel onto a nitrocellulose membrane using an electric current and fixed to the nitrocellulose and probed with antibodies against the target proteins (anti-PD and anti-ubiquitin; Invitrogen Ubi-1, Cat #13-1600). Bound antibody was revealed with horseradish peroxidase-conjugated rabbit anti-mouse antibody using enhanced chemiluminescence (Amersham Biosciences).
-
bioRxiv - Microbiology 2020Quote: ... The total protein content was measured using the Pierce BCA Protein Assay kit (Thermo Fisher) and 7.5 µg of lysate was run on a 4-12% Bis-Tris sodium dodecyl sulfate (SDS)-polyacrylamide gel with MOPS running buffer (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using BCA protein assay kit (ThermoFisher Scientific; Waltham, MA, United States) in accordance with manufacturer’s instructions and 10 μg of protein was loaded per lane (N = 3 - 4) ...
-
bioRxiv - Cancer Biology 2021Quote: One mg protein sample was precleared with Dynabeads Protein A (Thermo Fisher, UK, Cat.100002D) and 1 μg isotype IgG2a (Cell Signalling ...
-
bioRxiv - Cancer Biology 2021Quote: ... p53-associated protein were pulled down by 5 μl DynabeadsTM protein G beads (#10004D, Invitrogen) at 4 °C for 1-1.5 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined by a Pierce BCA protein assay (Life Technologies, #23225). Equal amounts of protein were loaded into 8% Bolt™ Bis-Tris Plus gels (Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was performed using a Pierce Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) and 300 μg of proteins were used for O-glycans enrichment adapted as previously described (25) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein levels were normalised across samples using a Pierce™ BCA protein assay kit (ThermoFisher) according to manufacturers instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was collected for protein concentration quantification with BCA Protein Assay Kit (ThermoFisher Scientific). An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system ...
-
bioRxiv - Cell Biology 2020Quote: ... the proteins were separated with the NuPAGE system (ThermoFisher, 4-12% Bis-Tris protein gel), and transferred to PVDF membrane (ThermoFisher) ...
-
bioRxiv - Cell Biology 2020Quote: ... and the protein concentration was determined using the BCA Protein Assay Kit (#23227, Thermo Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... Total protein concentration was measured using a Pierce BCA protein assay kit (Thermo Scientific Fisher). After boiling the samples in Laemmli buffer at 95°C for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) according to manufacturer protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined with the Pierce 660nm Protein Assay (Thermo Fisher Scientific, cat# 2262). Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were loaded in NuPAGE bis-tris protein gels (Thermo Fisher Scientific) and electroblotted to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2020Quote: ... The protein concentration was assessed using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). The mitochondria were pelleted by centrifugation (14,000 x g ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
-
bioRxiv - Microbiology 2019Quote: ... The proteins concentration was determined using a BCA Protein Assay Reagent Kit (Thermo Fisher Scientific) and stored at −80°C.
-
bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was quantified using the BCA Protein Assay Kit (Fisher Scientific, Cat# 23227). Samples were normalized to a fixed amount of protein ...
-
bioRxiv - Biochemistry 2022Quote: ... Total protein content was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... and 0.01% bromophenol blue) and protein concentrations were measured using Pierce 660nm Protein Assay (ThermoFisher).
-
bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations were measured using Pierce ™ BCA Protein Assay Kit (23225; Thermo Scientific) with a Albumin BSA Standards ranging from 2000 μg/mL to 0 μg/mL using a Nanodrop Spectrophotometer cuvette system (ND-2000C ...