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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells (wild-type or mS37 knock-out) were transfected in 6-well cell culture dish at 70% confluency with 2 μg plasmid using Lipofectamine 2000 (ThermoFisher scientific, Cat#11668027). Cells were collected after 72h ...
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bioRxiv - Neuroscience 2024Quote: Collected rosettes were plated onto a polyhema-treated 6-cm Petri dish in NDMB medium containing NDM and 2% B27 w/o vitamin A (Gibco, Grand Island, NY), supplemented with 10ng/ml IGF-1 ...
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bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were rinsed three times with 1X PBS and coated with ProLong Gold antifade mounting medium with DAPI (4’,6-diamidino-2-phenylindole, 100 ng/ml) (Life Technologies, P-36931) on slides ...
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bioRxiv - Bioengineering 2023Quote: ... These slices were then fixed in 4’,6-diamidino-2-phenylindole (DAPI) mounting media (Fluoromount-G® with DAPI, Thermo Fisher Scientific, USA), and washed twice with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then stained with 4′,6-diamidino-2-phenylindole (DAPI) to stain cell nuclei and mounted with Immu-Mount mountant (Thermo Scientific, Cat# 9990402) onto microscope slides.
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bioRxiv - Microbiology 2022Quote: ... and blocked for 45 min in 2% bovine serum albumin (BSA) in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 10 ^5 TC32 or A673 cells (mixed in a 1:1 solution of growth factor reduced Geltrex [Life Technologies, catalog number A1413202] with PBS) were injected into the mouse in a peritibial location.
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bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
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bioRxiv - Cell Biology 2021Quote: ... HT-1080 cells were additionally supplemented with 1% MEM nonessential amino acids (Cat# 11140-050, Life Technologies). 1X PBS (Cat# 97062-338 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% non-essential amino acids in 75 cm2 culture flasks (NUNC™, Roskilde Site, Kamstrupvej, Denmark) under humidified standard condition at 37 °C in 5% CO2 atmosphere (Forma water jacketed CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... plus 10% fetal bovine serum and 1 × MEM Non-Essential Amino Acids Solution (Life Technologies, # 11140-050).
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bioRxiv - Immunology 2022Quote: ... 50 µL/well of cold PBS 0.25% BSA with SYTO BC nucleic acid stain (1:500, ThermoFisher) was added ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were also stained to detect activity of tartrate-resistant acid phosphatase (TRAP) (1:80, Invitrogen, E6601A) and tissue non-specific alkaline phosphatase (TNAP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Standards were made with between 50μl and 1 ml of monosaccharide solution (MS) (1.8% galacturonic acid (ThermoFisher) in 0.1M sodium acetate (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was visualized with SYBRTMTM Gold Nucleic Acid Gel Stain (Invitrogen, 1:10,000 dilution in TBE-buffer). Gel containing fragments between 100-1,000 bp was cut and resuspended in Diffusion buffer (0.5 M CH3COONH4 ...
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bioRxiv - Immunology 2020Quote: ... or with SYBR® Green I Nucleic Acid Gel Stain (Invitrogen #S-7563, 1:1000 vol/vol) in flow cytometry buffer (100 µL ...
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bioRxiv - Microbiology 2020Quote: ... antibiotics (100U of penicillin/mL and 100μg of streptomycin/mL) and 1% non-essential amino acids (Gibco). Baby hamster kidney– 21 (BHK-21 ...
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bioRxiv - Immunology 2021Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). After the first passage the antibiotic concentration was reduced to 1%.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were digested in 1 mL trace metal grade nitric acid (Thermo Fisher, Waltham, MA; Catalog #A509) and 1 mL hydrogen peroxide (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Unlipidated C25SShhN cDNA and non-palmitoylated C25AShh cDNA (amino acids 1-438) were inserted into pcDNA3.1 (Invitrogen). Primer sequences can be provided upon request ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1% v/v MEM Non-Essential Amino Acids Solution 100X (Gibco, Life Technologies).
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bioRxiv - Pathology 2023Quote: ... followed by 7 min incubation with SYTOX green Nucleic Acid Stain (Invitrogen S7020; 1:700 in ddH20) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Glycans were fluorescently labeled with a 1.5:1 ratio of 50mM APTS (8-aminoinopyrene-1,3,6-trisulfonic acid, ThermoFisher) in 1.2M citric acid and 1M sodium cyanoborohydride in tetrahydrofuran (FisherScientific ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... and essential medium non-essential amino acids solution (diluted 1/100) (all from Invitrogen, Carlsbad, CA, USA). Cells were maintained at 37 °C in an atmosphere with 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... dissolved in 1% formic acid and loaded on a nanoflow HPLC system (Easy-nLC1000, Thermo Fisher Scientific) coupled to a Q Exactive (Q Exactive HF for collagen IV samples ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM freshly prepared ascorbic acid) with 1 μl Alexa Fluor™ 647 Azide (Thermo Fisher Scientific) per 5 ml was added to the cells ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1% v/v MEM Non-Essential Amino Acids Solution 100X (Gibco, Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
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bioRxiv - Cell Biology 2024Quote: ... Unlipidated C25SShhN cDNA and non-palmitoylated C25SShh cDNA (amino acids 1-438) were inserted into pcDNA3.1 (Invitrogen). Primer sequences can be provided upon request ...
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bioRxiv - Bioengineering 2024Quote: ... glass coverslips were submerged in piranha solution composed of 3:1 sulfuric acid (H2SO4, Fisher Scientific, A300) and 30 wt% hydrogen peroxide (H2O2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The labelling reaction was quenched by diluting the samples with 1% trifluoroacetic acid (TFA; Thermo Fisher Scientific), 0.4 M TCEP ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1% minimum essential medium non-essential amino acids (MEM NEAA; Thermo Scientific, Gibco, Cat. #11140) and 1% Penicillin-Streptomycin (Thermo Scientific ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1% minimum essential medium non-essential amino acids (MEM NEAA; Thermo Scientific, Gibco, Cat. #11140) and 1% Penicillin-Streptomycin (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... serum-free medium (DMEM:F12 at 2:1) supplemented with N2 (1% v/v; Invitrogen), 1mM L-glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated wildtype and Ncan KO cortical neurons were initially infected with Control and the two Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Biochemistry 2024Quote: ... Solutions were further diluted to 1 µM using 2:1 MeOH:CHCl3 (ThermoFisher Scientific, Australia) with 5 mM ammonium acetate (SigmaAldrich ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated cortical neurons were infected with Control and Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa647 ICAM-2 at 1:100 and Live/Dead at 1:1000 (L34962, Invitrogen), for sorting of CD45neg ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 2-mercaptoethanol and 1 mM PMSF) by sonication (using a Fisher Scientific Sonic Dismembrator with a Qsonica Ultrasonic Sonicator converter ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen, Cat. L3000015). Viral supernatants were collected 48 hours after transfection and filtered through a 0.45-μm membrane (Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were selected with 1 μg ml-1 and 2 μg ml-1 puromycin (Thermo Fisher Scientific) respectively for 10 days.
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected with 1 µg mL-1 and 2 µg mL-1 puromycin (Thermo Fisher Scientific), respectively.
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...