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Citations for Thermo Fisher :
4051 - 4100 of 6235 citations for Serpin B6 Human Trx His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Human embryonic kidney 293T cells were grown in Complete DMEM (Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 8 µg of cDNA was fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Hepatic gene expression was measured using Affymetrix Human Exon 1.0 ST v1 Array (Affymetrix, Santa Clara, CA) in adolescents and Illumina Human-6 Expression Bead Chips (Illumina ...
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bioRxiv - Bioengineering 2022Quote: Intracellular mitochondrial presence within human heart organoids was visualized using Mitotracker Deep Red FM (Thermo Fisher Scientific). Mitotracker was prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Calcium transient activity within the human heart organoids was assessed using Fluo-4 AM (Thermo Fisher Scientific). Fluo-4 AM was solubilized in DMSO per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...
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bioRxiv - Biochemistry 2023Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA) ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... HCC1395 and SF295 human cancer cell lines were grown in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA) medium supplemented with 10% FBS and 50 units/ml of penicillin/streptomycin.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
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bioRxiv - Immunology 2023Quote: The human promyelocytic leukemia HL-60 cell line (ATCC #CCL-240) was maintained in IMDM media (Gibco) with 10% heat-inactivated (HI ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
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bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
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bioRxiv - Immunology 2023Quote: The human myelogenous leukemia cell line K562 was cultured in Roswell Park Memorial Institute (RPMI) media (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: Both plasmids encoding for the LC and HC were transfected into Expi293F human cells (Thermo Fisher Scientific) with purified DNA and polyethylenimine (PEI 1:3) ...
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bioRxiv - Cancer Biology 2023Quote: ... SW837 (human colon cells, ATCC CCL-235) were grown in Leibovitz’s L-15 medium (Gibco #11415-049) with 10% FCS (Hyclone #SH30084.03n ...
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bioRxiv - Cell Biology 2023Quote: Normal human epidermal keratinocytes (Kurabo) from Caucasian neonatal foreskin were cultured with EpiLife medium (Thermo Fisher Scientific) supplemented with 60 µM CaCl2 and HuMedia KG growth factor kit (Kurabo) ...
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bioRxiv - Genomics 2022Quote: ... CD4+ T cells were isolated by magnetic negative selection using Untouched Human CD4+ Isolation Kit (Invitrogen 11346D) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA ...
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bioRxiv - Microbiology 2023Quote: ... human actin mRNA and EMCV viral RNA was performed mixing the Fast SYBR green Master Mix (ThermoFisher) with cDNA and 1µM of the forward and reverse corresponding primers ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were incubated with PBS solution containing various concentrations of human plasma fibronectin (Thermo Fisher Scientific, US) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) before adding the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
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bioRxiv - Genomics 2023Quote: ... KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Human cervix adenocarcinoma epithelial HeLa 229 cells (ATCC CCL-2.1) were cultured in RPMI 1640 medium (Gibco) containing 5% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D737 infected erythrocytes were cultured in O positive human erythrocytes in RPMI 1640 media (Gibco, USA) containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... version 46) and humans using SEQUEST and Mascot (Version 2.4.1) via Proteome Discoverer v2.2 (Thermo Fisher Scientific). Trypsin was selected as the protease of choice with a maximum of two missed cleavages ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 human monocytes (ATCC) were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640, Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with Hu5F9 or TJC4 and then stained with labeled anti-human secondary antibody (Invitrogen). B6H12 was acquired from Bio X Cell ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then incubated with primary mouse monoclonal anti-human cardiac troponin T (Thermo Fisher Scientific) or mouse monoclonal anti-human CD3 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using the Affymetrix GeneChip® Human Clariom S Array (Thermo Fisher Scientific), including more than 210,000 distinct probes representative of 21,448 annotated genes (Genome Reference Consortium Human Build 38 (GRCh38) ...
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bioRxiv - Microbiology 2023Quote: ... at an interval of 24 hr with siRNAs targeting human PKR/EIF2AK2 (Assay ID 142330-Thermofisher scientific) or human TIA-1 (Assay ID 139893-Thermofisher scientific ...
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bioRxiv - Neuroscience 2023Quote: Measurement of Aβ1-40 levels was performed using an Amyloid beta 40 Human ELISA Kit (Invitrogen, KHB3482). For further information please see Supplementary Material.
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Immunology 2023Quote: ... A goat anti-Human IgG secondary antibody labelled with Alexa Fluor 488 was added (Thermo Fisher Scientific). The plates were incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-labeled goat anti-human IgG secondary antibody (Invitrogen, A18817) at 37 °C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Anti-mouse ZAKa antibody (proteintech, #14945-1-AP) and anti-human ZAKa antibody were from Thermo Fisher Scientific (A301-993A) ...
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bioRxiv - Immunology 2023Quote: ... Cytokine release in the supernatants was measured with an Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen) and was analyzed with the DropArray (Curiox Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and S-D614G-N149Q were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using a HisTrap FF column (GE Life Sciences)(64 ...
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bioRxiv - Microbiology 2023Quote: ... Secreted antibodies were detected with 1µg/mL goat anti-human IgG (H+L)-Alexa Fluor 594 (Invitrogen), which was added to the cell culture media used to resuspend the antigen-coated beads.