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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for Recombinant Human PRSS22 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and human HEK293T cells was extracted using TRIzol reagent (Invitrogen). Endogenous RNase was further removed by ethanol precipitation ...
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bioRxiv - Cell Biology 2023Quote: ... HEK 293FT human embryonic kidney cells (Thermo Fisher Scientific, #R70007) were cultured in DMEM (Corning ...
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bioRxiv - Physiology 2024Quote: Human islets were cultured in CMRL medium (Gibco 11530-037) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs were purchased from Life Technologies (for human cell lines) and from Horizon Discovery (for human PCLS) ...
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bioRxiv - Genomics 2024Quote: 10 ng of fully methylated human blood (Thermo Scientific, SD1131) and Jurkat (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... human neutrophils were labeled with calcein AM (Thermo Fisher Scientific) and left to migrate for 2 hours before termination of the experiment ...
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bioRxiv - Bioengineering 2024Quote: ... Research-grade DynabeadsTM Human T-Expander CD3/CD28 beads (Gibco) were washed using DynaMag-2 magnet (Gibco ...
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bioRxiv - Synthetic Biology 2024Quote: Rabbit polyclonal anti-Human ASGR1 antibody (Thermo Fisher, PA5-80356), 1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 ng/ml human bFGF (PHG0021, Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2024Quote: ... goat anti human IgG (H+L) HRP (Invitrogen, Cat#31412) was added to wells at a 1:10,000 dilution for one hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: Gibco Human Astrocytes (cat. nr. K1884, Lot nr. 802268, Invitrogen) were sourced from a 20 weeks old male donor ...
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bioRxiv - Cell Biology 2024Quote: ... A human IL-6 ELISA kit (Thermo Fisher Scientific, KHC0061) was then used as instructed by the manufacturer to measure IL-6 concentrations ...
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bioRxiv - Neuroscience 2024Quote: ... Luminex assay was performed using ProcartaPlex Human Basic Kit (Invitrogen) and a panel of ProcartaPlex Human Simplex (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed twice with human wash buffer (DMEM/F12 (Gibco), 10 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... or a human NT3 ELISA kit (Thermo Fisher Scientific, EHNTF3). Both ELISAs were performed as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CAFs were kept in Human Plasma-Like Medium (HPLM, Gibco) with ascorbic acid for 11 days ...
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bioRxiv - Immunology 2024Quote: ... or anti-human IgG Alexa Fluor 647 conjugate (Invitrogen, A21445) or Streptavidin Alexa Fluor 647 conjugate (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads Human T-Activator CD3/CD28 (11161D; Gibco; 1:100) or plate bound with anti-CD3 and anti-CD28 antibodies (0.5µg/ml each ...
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bioRxiv - Cell Biology 2019Quote: ... washed once with PBS and mixed with a total of 10 μg recombinant DNA in electroporation buffer R (Invitrogen). Cells were singularly electroshocked at 1600mV for 20 msec by placing the electroporation tip into the column filled by E2 buffer (following manufacturer specifications) ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were cultured for 9 to 12 days in an erythroblast enrichment medium [38] to expand the erythroblast population and 2.5×105 cells were transduced using recombinant Sendai viral vectors (Cytotune-iPSC 2.0TM, Thermofisher scientific), expressing the four reprogramming factors Oct4 ...
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bioRxiv - Microbiology 2019Quote: The PfCHC-2xFKBP-GFP soluble extract in buffer B was incubated with 4μl recombinant anti-GFP nanobodies covalently coupled to surface-activated Epoxy magnetic beads (Dynabeads M270 Epoxy, ThermoFisher) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were purchased from Kerafast and grown in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant epidermal growth factor (Gibco, #37010022) with 1% Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant lentiviral and retroviral vectors produced from previous steps were amplified using the GeneJET Plasmid Maxiprep Kit (Thermo Scientific). The 3rd generation lentiviral (pRSV.REV ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Cell Biology 2020Quote: ... containing the two recombinant Gateway sites attB1.1 and attbB.1 and subcloned into a pDONR/Zeo Gateway Donor vector (Invitrogen) as the entry clones ...
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bioRxiv - Cell Biology 2021Quote: ... Ift81 and Wdr35 were amplified by PCR with oligos carrying the recombinant sites and cloned into pcDNA3.1(+) vector (Thermofisher) in which a FLAG or HA epiptope was introduced prior to the multicloning site ...
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bioRxiv - Systems Biology 2022Quote: ... and recombinant synthetic genes fused to the GFP-coding sequence at the 3-termini were ordered from GeneArt (ThermoFisher). Genes were further cloned into pDUAL-PuroR lentivectors (Escors et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Cell Biology 2022Quote: All recombinant condensin complexes were expressed using insect cell strains from the Bac-to- Bac Baculovirus Expression System (ThermoFisher) as previously described (Kinoshita et al. ...
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bioRxiv - Plant Biology 2021Quote: The recombinant vectors were then transferred to Agrobacterium tumefaciens ElectroMAX™ LBA4404 cells by electroporation (Invitrogen, Carlsbad, CA, USA). The expression vector in A ...
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bioRxiv - Immunology 2020Quote: ... and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells ...
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... Generation of bacmid DNA and recombinant baculovirus was performed according to protocols from the Bac-to-Bac system (Invitrogen). All secreted proteins were purified from culture supernatant using streptactin beads (IBA ...
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bioRxiv - Molecular Biology 2022Quote: ... these plasmids were transformed into DH10MultiBac electrocompetent cells and recombinant baculoviruses produced according to manufacturer’s instructions (Bac-to-Bac expression system, Invitrogen). All constructs were verified by sequencing (VK8 or VK10/WA2 ...
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bioRxiv - Cell Biology 2022Quote: All recombinant Cdh23 variants for in-vitro studies were expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific), following the prescribed protocol for transfection in ExpiCHO cells ...
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bioRxiv - Cancer Biology 2019Quote: ... The following additional niche factors were added to generate “Full” media: 50 ng/ml mouse recombinant EGF (Life Technologies), 100 ng/ml mouse recombinant Noggin (Peprotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transfected with recombinant lentiCRISPRv2 together with psPAX2 and pMD2.G using Lipofectamine 2000 (Thermo Fisher Scientific). Undigested lentiCRISPRv2 plasmid lacking sgRNA sequence was used for pseudovirus production as a control ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... the nucleotide sequence was inserted by means of a BP recombinant reaction (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol into a pDONR221 donor vector ...
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bioRxiv - Microbiology 2020Quote: ... at day 0 the animals were subcutaneously administered either approximately 10,000,000 fffus of detergent-inactivated UHV or 0.1 mg of recombinant UHV NP emulsified in Freund’s incomplete adjuvant (ThermoFisher Scientific), the total volume per individual was 125 μl ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with recombinant plasmid p3xFLAG-NS5A or NS5A mutant expression vectors using Lipo3000 (Thermo Fisher Scientific, USA) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted using the hot-phenol method (Schmitt et al., 1990) and treated with recombinant DnaseI (Ambion). For real-time reverse transcription PCR ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Immunology 2022Quote: ... recombinant GBPs were incubated with Alexa Fluor 488 C5 maleimide dye or Alexa Fluor 647 C2 maleimide dye (Invitrogen) in a ratio of 1:1 or 1:2 on ice for 10–20 min ...
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bioRxiv - Neuroscience 2023Quote: Sf9 cells for generation of baculovirus and expression of recombinant antibody fragment are from Thermo Fisher (12659017, lot 421973). The cells were not authenticated experimentally for these studies ...
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bioRxiv - Physiology 2023Quote: A recombinant adenovirus expressing mouse Sult2b1 (Ad-Sult2b1) was produced using the ViraPower Adenoviral Expression System (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Endpoint PCR for all mouse CASK experiments was performed using 0.25 U recombinant Taq DNA Polymerase (Thermo Fisher Scientific) with 2 mM MgCl2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...